Cargando…

Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases

Sporotrichosis has been expanding throughout the Brazilian territory in recent years. New outbreaks have emerged, and consequently, the sporotrichosis agents, mainly Sporothrix brasiliensis, should remain in the environment somehow. Therefore, the aim of this study was to investigate the presence of...

Descripción completa

Detalles Bibliográficos
Autores principales: Rabello, Vanessa Brito Souza, Almeida-Silva, Fernando, Scramignon-Costa, Bruno de Souza, Motta, Beatriz da Silva, de Macedo, Priscila Marques, Teixeira, Marcus de Melo, Almeida-Paes, Rodrigo, Irinyi, Laszlo, Meyer, Wieland, Zancopé-Oliveira, Rosely Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134204/
https://www.ncbi.nlm.nih.gov/pubmed/35646737
http://dx.doi.org/10.3389/fcimb.2022.894297
_version_ 1784713743084552192
author Rabello, Vanessa Brito Souza
Almeida-Silva, Fernando
Scramignon-Costa, Bruno de Souza
Motta, Beatriz da Silva
de Macedo, Priscila Marques
Teixeira, Marcus de Melo
Almeida-Paes, Rodrigo
Irinyi, Laszlo
Meyer, Wieland
Zancopé-Oliveira, Rosely Maria
author_facet Rabello, Vanessa Brito Souza
Almeida-Silva, Fernando
Scramignon-Costa, Bruno de Souza
Motta, Beatriz da Silva
de Macedo, Priscila Marques
Teixeira, Marcus de Melo
Almeida-Paes, Rodrigo
Irinyi, Laszlo
Meyer, Wieland
Zancopé-Oliveira, Rosely Maria
author_sort Rabello, Vanessa Brito Souza
collection PubMed
description Sporotrichosis has been expanding throughout the Brazilian territory in recent years. New outbreaks have emerged, and consequently, the sporotrichosis agents, mainly Sporothrix brasiliensis, should remain in the environment somehow. Therefore, the aim of this study was to investigate the presence of Sporothrix spp. in the environment from an area of ​​the Rio de Janeiro state, Brazil, with recurrent cases of human and animal sporotrichosis. Abandoned demolition timber wood samples were collected in the garden of a house where the cases of human and feline sporotrichosis have occurred in the last 10 years. The environmental survey revealed a Sporothrix spp. colony from the serial dilution cultures of one abandoned demolition wood sample. In addition, a fungal strain isolated from a cat with skin lesions that lived in the house was also included in the study. The species-specific PCR, and calmodulin partial sequencing identified the environmental and cat isolates as S. brasiliensis. Furthermore, the phylogenetic analysis performed with the partial sequences of internal transcribed spacer region and constitutive genes (calmodulin, β-tubulin, and chitin synthase) showed high similarity between environmental and cat isolates from the same geographic region. Moreover, the antifungal susceptibility test revealed that the minimal inhibitory concentration of itraconazole from the environment isolate was lower than the cat isolate, while amphotericin B and terbinafine were similar. Our results show that S. brasiliensis is able to maintain itself in the environmental material for years. With this, we corroborate that the eco-epidemiology of sporotrichosis is not well understood, and despite the major occurrence of S. brasiliensis in Brazil, it is rarely isolated from the environment.
format Online
Article
Text
id pubmed-9134204
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91342042022-05-27 Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases Rabello, Vanessa Brito Souza Almeida-Silva, Fernando Scramignon-Costa, Bruno de Souza Motta, Beatriz da Silva de Macedo, Priscila Marques Teixeira, Marcus de Melo Almeida-Paes, Rodrigo Irinyi, Laszlo Meyer, Wieland Zancopé-Oliveira, Rosely Maria Front Cell Infect Microbiol Cellular and Infection Microbiology Sporotrichosis has been expanding throughout the Brazilian territory in recent years. New outbreaks have emerged, and consequently, the sporotrichosis agents, mainly Sporothrix brasiliensis, should remain in the environment somehow. Therefore, the aim of this study was to investigate the presence of Sporothrix spp. in the environment from an area of ​​the Rio de Janeiro state, Brazil, with recurrent cases of human and animal sporotrichosis. Abandoned demolition timber wood samples were collected in the garden of a house where the cases of human and feline sporotrichosis have occurred in the last 10 years. The environmental survey revealed a Sporothrix spp. colony from the serial dilution cultures of one abandoned demolition wood sample. In addition, a fungal strain isolated from a cat with skin lesions that lived in the house was also included in the study. The species-specific PCR, and calmodulin partial sequencing identified the environmental and cat isolates as S. brasiliensis. Furthermore, the phylogenetic analysis performed with the partial sequences of internal transcribed spacer region and constitutive genes (calmodulin, β-tubulin, and chitin synthase) showed high similarity between environmental and cat isolates from the same geographic region. Moreover, the antifungal susceptibility test revealed that the minimal inhibitory concentration of itraconazole from the environment isolate was lower than the cat isolate, while amphotericin B and terbinafine were similar. Our results show that S. brasiliensis is able to maintain itself in the environmental material for years. With this, we corroborate that the eco-epidemiology of sporotrichosis is not well understood, and despite the major occurrence of S. brasiliensis in Brazil, it is rarely isolated from the environment. Frontiers Media S.A. 2022-05-12 /pmc/articles/PMC9134204/ /pubmed/35646737 http://dx.doi.org/10.3389/fcimb.2022.894297 Text en Copyright © 2022 Rabello, Almeida-Silva, Scramignon-Costa, Motta, de Macedo, Teixeira, Almeida-Paes, Irinyi, Meyer and Zancopé-Oliveira https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Rabello, Vanessa Brito Souza
Almeida-Silva, Fernando
Scramignon-Costa, Bruno de Souza
Motta, Beatriz da Silva
de Macedo, Priscila Marques
Teixeira, Marcus de Melo
Almeida-Paes, Rodrigo
Irinyi, Laszlo
Meyer, Wieland
Zancopé-Oliveira, Rosely Maria
Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_full Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_fullStr Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_full_unstemmed Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_short Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_sort environmental isolation of sporothrix brasiliensis in an area with recurrent feline sporotrichosis cases
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134204/
https://www.ncbi.nlm.nih.gov/pubmed/35646737
http://dx.doi.org/10.3389/fcimb.2022.894297
work_keys_str_mv AT rabellovanessabritosouza environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT almeidasilvafernando environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT scramignoncostabrunodesouza environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT mottabeatrizdasilva environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT demacedopriscilamarques environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT teixeiramarcusdemelo environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT almeidapaesrodrigo environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT irinyilaszlo environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT meyerwieland environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases
AT zancopeoliveiraroselymaria environmentalisolationofsporothrixbrasiliensisinanareawithrecurrentfelinesporotrichosiscases