Cargando…

Autonomous Treatment of Bacterial Infections in Vivo Using Antimicrobial Micro- and Nanomotors

[Image: see text] The increasing resistance of bacteria to existing antibiotics constitutes a major public health threat globally. Most current antibiotic treatments are hindered by poor delivery to the infection site, leading to undesired off-target effects and drug resistance development and sprea...

Descripción completa

Detalles Bibliográficos
Autores principales: Arqué, Xavier, Torres, Marcelo D. T., Patiño, Tania, Boaro, Andreia, Sánchez, Samuel, de la Fuente-Nunez, Cesar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134509/
https://www.ncbi.nlm.nih.gov/pubmed/35486889
http://dx.doi.org/10.1021/acsnano.1c11013
_version_ 1784713791834947584
author Arqué, Xavier
Torres, Marcelo D. T.
Patiño, Tania
Boaro, Andreia
Sánchez, Samuel
de la Fuente-Nunez, Cesar
author_facet Arqué, Xavier
Torres, Marcelo D. T.
Patiño, Tania
Boaro, Andreia
Sánchez, Samuel
de la Fuente-Nunez, Cesar
author_sort Arqué, Xavier
collection PubMed
description [Image: see text] The increasing resistance of bacteria to existing antibiotics constitutes a major public health threat globally. Most current antibiotic treatments are hindered by poor delivery to the infection site, leading to undesired off-target effects and drug resistance development and spread. Here, we describe micro- and nanomotors that effectively and autonomously deliver antibiotic payloads to the target area. The active motion and antimicrobial activity of the silica-based robots are driven by catalysis of the enzyme urease and antimicrobial peptides, respectively. These antimicrobial motors show micromolar bactericidal activity in vitro against different Gram-positive and Gram-negative pathogenic bacterial strains and act by rapidly depolarizing their membrane. Finally, they demonstrated autonomous anti-infective efficacy in vivo in a clinically relevant abscess infection mouse model. In summary, our motors combine navigation, catalytic conversion, and bactericidal capacity to deliver antimicrobial payloads to specific infection sites. This technology represents a much-needed tool to direct therapeutics to their target to help combat drug-resistant infections.
format Online
Article
Text
id pubmed-9134509
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-91345092022-05-27 Autonomous Treatment of Bacterial Infections in Vivo Using Antimicrobial Micro- and Nanomotors Arqué, Xavier Torres, Marcelo D. T. Patiño, Tania Boaro, Andreia Sánchez, Samuel de la Fuente-Nunez, Cesar ACS Nano [Image: see text] The increasing resistance of bacteria to existing antibiotics constitutes a major public health threat globally. Most current antibiotic treatments are hindered by poor delivery to the infection site, leading to undesired off-target effects and drug resistance development and spread. Here, we describe micro- and nanomotors that effectively and autonomously deliver antibiotic payloads to the target area. The active motion and antimicrobial activity of the silica-based robots are driven by catalysis of the enzyme urease and antimicrobial peptides, respectively. These antimicrobial motors show micromolar bactericidal activity in vitro against different Gram-positive and Gram-negative pathogenic bacterial strains and act by rapidly depolarizing their membrane. Finally, they demonstrated autonomous anti-infective efficacy in vivo in a clinically relevant abscess infection mouse model. In summary, our motors combine navigation, catalytic conversion, and bactericidal capacity to deliver antimicrobial payloads to specific infection sites. This technology represents a much-needed tool to direct therapeutics to their target to help combat drug-resistant infections. American Chemical Society 2022-04-29 2022-05-24 /pmc/articles/PMC9134509/ /pubmed/35486889 http://dx.doi.org/10.1021/acsnano.1c11013 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Arqué, Xavier
Torres, Marcelo D. T.
Patiño, Tania
Boaro, Andreia
Sánchez, Samuel
de la Fuente-Nunez, Cesar
Autonomous Treatment of Bacterial Infections in Vivo Using Antimicrobial Micro- and Nanomotors
title Autonomous Treatment of Bacterial Infections in Vivo Using Antimicrobial Micro- and Nanomotors
title_full Autonomous Treatment of Bacterial Infections in Vivo Using Antimicrobial Micro- and Nanomotors
title_fullStr Autonomous Treatment of Bacterial Infections in Vivo Using Antimicrobial Micro- and Nanomotors
title_full_unstemmed Autonomous Treatment of Bacterial Infections in Vivo Using Antimicrobial Micro- and Nanomotors
title_short Autonomous Treatment of Bacterial Infections in Vivo Using Antimicrobial Micro- and Nanomotors
title_sort autonomous treatment of bacterial infections in vivo using antimicrobial micro- and nanomotors
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134509/
https://www.ncbi.nlm.nih.gov/pubmed/35486889
http://dx.doi.org/10.1021/acsnano.1c11013
work_keys_str_mv AT arquexavier autonomoustreatmentofbacterialinfectionsinvivousingantimicrobialmicroandnanomotors
AT torresmarcelodt autonomoustreatmentofbacterialinfectionsinvivousingantimicrobialmicroandnanomotors
AT patinotania autonomoustreatmentofbacterialinfectionsinvivousingantimicrobialmicroandnanomotors
AT boaroandreia autonomoustreatmentofbacterialinfectionsinvivousingantimicrobialmicroandnanomotors
AT sanchezsamuel autonomoustreatmentofbacterialinfectionsinvivousingantimicrobialmicroandnanomotors
AT delafuentenunezcesar autonomoustreatmentofbacterialinfectionsinvivousingantimicrobialmicroandnanomotors