Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat

The present study aimed to investigate the expression of mRNA in the brachial plexus avulsion neuropathic pain model and analyze biological functions. Microarray mRNA assay and reverse transcriptase quantitative polymerase chain reaction (RT-PCR) were conducted. The whole blood was collected from tw...

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Autores principales: Wang, Le, Lao, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134582/
https://www.ncbi.nlm.nih.gov/pubmed/35619085
http://dx.doi.org/10.1186/s12868-022-00717-9
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author Wang, Le
Lao, Jie
author_facet Wang, Le
Lao, Jie
author_sort Wang, Le
collection PubMed
description The present study aimed to investigate the expression of mRNA in the brachial plexus avulsion neuropathic pain model and analyze biological functions. Microarray mRNA assay and reverse transcriptase quantitative polymerase chain reaction (RT-PCR) were conducted. The whole blood was collected from two groups for Microarray mRNA analysis. The predicted mRNA targets were studied by gene ontology analysis and pathway analysis. We identified 3 targeted mRNAs, including PIK3CB, HRAS, and JUN. The results showed that PIK3CB, HRAS, and JUN gene expression was increased in the control group but decreased in the neuropathic pain group. These findings indicate that certain genes may be important biomarkers for the potential targets for the prevention and treatment of brachial plexus avulsion caused neuropathic pain.
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spelling pubmed-91345822022-05-27 Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat Wang, Le Lao, Jie BMC Neurosci Research The present study aimed to investigate the expression of mRNA in the brachial plexus avulsion neuropathic pain model and analyze biological functions. Microarray mRNA assay and reverse transcriptase quantitative polymerase chain reaction (RT-PCR) were conducted. The whole blood was collected from two groups for Microarray mRNA analysis. The predicted mRNA targets were studied by gene ontology analysis and pathway analysis. We identified 3 targeted mRNAs, including PIK3CB, HRAS, and JUN. The results showed that PIK3CB, HRAS, and JUN gene expression was increased in the control group but decreased in the neuropathic pain group. These findings indicate that certain genes may be important biomarkers for the potential targets for the prevention and treatment of brachial plexus avulsion caused neuropathic pain. BioMed Central 2022-05-26 /pmc/articles/PMC9134582/ /pubmed/35619085 http://dx.doi.org/10.1186/s12868-022-00717-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Le
Lao, Jie
Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat
title Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat
title_full Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat
title_fullStr Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat
title_full_unstemmed Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat
title_short Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat
title_sort microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134582/
https://www.ncbi.nlm.nih.gov/pubmed/35619085
http://dx.doi.org/10.1186/s12868-022-00717-9
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