Cargando…

Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)

Cross-pollination can improve the percentage of fruit set and fruit weight for most red flesh varieties in pitaya. The technology of pollen storage was very important for successful cross-pollination. However, till present, the technology of pollen storage is unsatisfactory in pitaya production. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Dai, Hong-fen, Jiang, Biao, Zhao, Jun-sheng, Li, Jun-cheng, Sun, Qing-ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134753/
https://www.ncbi.nlm.nih.gov/pubmed/35646022
http://dx.doi.org/10.3389/fpls.2022.866588
_version_ 1784713824487604224
author Dai, Hong-fen
Jiang, Biao
Zhao, Jun-sheng
Li, Jun-cheng
Sun, Qing-ming
author_facet Dai, Hong-fen
Jiang, Biao
Zhao, Jun-sheng
Li, Jun-cheng
Sun, Qing-ming
author_sort Dai, Hong-fen
collection PubMed
description Cross-pollination can improve the percentage of fruit set and fruit weight for most red flesh varieties in pitaya. The technology of pollen storage was very important for successful cross-pollination. However, till present, the technology of pollen storage is unsatisfactory in pitaya production. In this study, pitaya pollen stored at low temperature was taken as the research object, and its physicochemical indexes, metabolomics, and transcriptomics were studied. The results showed that in vitro pollen germination rate decreased significantly with the increase in storage time. Soluble sugar and soluble protein content of pollen peaked on the first day of storage, whereas its relative conductivity, and manlondialdehyde (MDA) and proline contents increased gradually during storage. At the same time, the antioxidant enzyme system of pollen was also affected. Superoxide dismutase (SOD) activity decreased, while the activities of catalase (CAT) and peroxidase (POD) increased and superoxide anion generation rate increased gradually during storage. According to the metabolomics results, amino acid, peptide, nucleotide, plant hormone, terpene, alcohol, phenol, flavonoid, sterol, vitamin, ester, sphingolipid, and ketone contents increased significantly during storage, whereas flavonoid and pigment contents declined gradually. During pollen storage, the gene expressions related to carbohydrate metabolism, protein metabolism, acid and lipid metabolism, sterol metabolism, plant hormone metabolism, and signal transductions were significantly downregulated. With KEGG pathway analysis, isoquinoline alkaloid biosynthesis, tyrosine metabolism, alanine, aspartate, and glutamate metabolism of pollen were affected significantly during low-temperature storage. Correlation analysis showed that the gene expression patterns of HuRP2, HuUPL1, and HuAAT2 had significant effects on pollen germination. D-arabinose 5-phosphate and myricetin were positively correlated with pollen germination rate, which was valuable for studying preservation agents. In this study, the changes in pollen during low-temperature storage were described from the level of metabolites and genes, which could provide theoretical support for the research and development of pollen long-term storage technology in pitaya.
format Online
Article
Text
id pubmed-9134753
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91347532022-05-27 Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus) Dai, Hong-fen Jiang, Biao Zhao, Jun-sheng Li, Jun-cheng Sun, Qing-ming Front Plant Sci Plant Science Cross-pollination can improve the percentage of fruit set and fruit weight for most red flesh varieties in pitaya. The technology of pollen storage was very important for successful cross-pollination. However, till present, the technology of pollen storage is unsatisfactory in pitaya production. In this study, pitaya pollen stored at low temperature was taken as the research object, and its physicochemical indexes, metabolomics, and transcriptomics were studied. The results showed that in vitro pollen germination rate decreased significantly with the increase in storage time. Soluble sugar and soluble protein content of pollen peaked on the first day of storage, whereas its relative conductivity, and manlondialdehyde (MDA) and proline contents increased gradually during storage. At the same time, the antioxidant enzyme system of pollen was also affected. Superoxide dismutase (SOD) activity decreased, while the activities of catalase (CAT) and peroxidase (POD) increased and superoxide anion generation rate increased gradually during storage. According to the metabolomics results, amino acid, peptide, nucleotide, plant hormone, terpene, alcohol, phenol, flavonoid, sterol, vitamin, ester, sphingolipid, and ketone contents increased significantly during storage, whereas flavonoid and pigment contents declined gradually. During pollen storage, the gene expressions related to carbohydrate metabolism, protein metabolism, acid and lipid metabolism, sterol metabolism, plant hormone metabolism, and signal transductions were significantly downregulated. With KEGG pathway analysis, isoquinoline alkaloid biosynthesis, tyrosine metabolism, alanine, aspartate, and glutamate metabolism of pollen were affected significantly during low-temperature storage. Correlation analysis showed that the gene expression patterns of HuRP2, HuUPL1, and HuAAT2 had significant effects on pollen germination. D-arabinose 5-phosphate and myricetin were positively correlated with pollen germination rate, which was valuable for studying preservation agents. In this study, the changes in pollen during low-temperature storage were described from the level of metabolites and genes, which could provide theoretical support for the research and development of pollen long-term storage technology in pitaya. Frontiers Media S.A. 2022-05-12 /pmc/articles/PMC9134753/ /pubmed/35646022 http://dx.doi.org/10.3389/fpls.2022.866588 Text en Copyright © 2022 Dai, Jiang, Zhao, Li and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Dai, Hong-fen
Jiang, Biao
Zhao, Jun-sheng
Li, Jun-cheng
Sun, Qing-ming
Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)
title Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)
title_full Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)
title_fullStr Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)
title_full_unstemmed Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)
title_short Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Hylocereus polyrhizus)
title_sort metabolomics and transcriptomics analysis of pollen germination response to low-temperature in pitaya (hylocereus polyrhizus)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134753/
https://www.ncbi.nlm.nih.gov/pubmed/35646022
http://dx.doi.org/10.3389/fpls.2022.866588
work_keys_str_mv AT daihongfen metabolomicsandtranscriptomicsanalysisofpollengerminationresponsetolowtemperatureinpitayahylocereuspolyrhizus
AT jiangbiao metabolomicsandtranscriptomicsanalysisofpollengerminationresponsetolowtemperatureinpitayahylocereuspolyrhizus
AT zhaojunsheng metabolomicsandtranscriptomicsanalysisofpollengerminationresponsetolowtemperatureinpitayahylocereuspolyrhizus
AT lijuncheng metabolomicsandtranscriptomicsanalysisofpollengerminationresponsetolowtemperatureinpitayahylocereuspolyrhizus
AT sunqingming metabolomicsandtranscriptomicsanalysisofpollengerminationresponsetolowtemperatureinpitayahylocereuspolyrhizus