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Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii

Powdery mildew (PM) caused by Podosphaera xanthii poses a continuous threat to the performance and yield of the cucumber (Cucumis sativus L.). Control in the initial stages of infection is particularly important. Here, we studied the differential physiological and transcriptomic changes between PM-r...

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Autores principales: Meng, Xiangnan, Yu, Yongbo, Song, Tiefeng, Yu, Yang, Cui, Na, Ma, Zhangtong, Chen, Lijie, Fan, Haiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134894/
https://www.ncbi.nlm.nih.gov/pubmed/35645993
http://dx.doi.org/10.3389/fpls.2022.872218
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author Meng, Xiangnan
Yu, Yongbo
Song, Tiefeng
Yu, Yang
Cui, Na
Ma, Zhangtong
Chen, Lijie
Fan, Haiyan
author_facet Meng, Xiangnan
Yu, Yongbo
Song, Tiefeng
Yu, Yang
Cui, Na
Ma, Zhangtong
Chen, Lijie
Fan, Haiyan
author_sort Meng, Xiangnan
collection PubMed
description Powdery mildew (PM) caused by Podosphaera xanthii poses a continuous threat to the performance and yield of the cucumber (Cucumis sativus L.). Control in the initial stages of infection is particularly important. Here, we studied the differential physiological and transcriptomic changes between PM-resistant strain B21-a-2-1-2 and PM-susceptible strain B21-a-2-2-2 at the early stage of P. xanthii attack. When challenged with P. xanthii, the tolerant line can postpone the formation of the pathogen primary germ. Comparative transcriptomic analysis suggested that DEGs related to the cell wall and to pathogen and hormone responses were similar enriched in both cucumber lines under P. xanthii infection. Notably, the number of DEGs triggered by P. xanthii in B21-a-2-1-2 was quintuple that in B21-a-2-2-2, revealing that the success of defense of resistant cucumber is due to rapidly mobilizing multiple responses. The unique responses detected were genes related to SA signaling, MAPK signaling, and Dof and WRKY transcription factors. Furthermore, 5 P. xanthii -inducible hub genes were identified, including GLPK, ILK1, EIN2, BCDHβ1, and RGGA, which are considered to be key candidate genes for disease control. This study combined multiple analytical approaches to capture potential molecular players and will provide key resources for developing cucumber cultivars resistant to pathogen stress.
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spelling pubmed-91348942022-05-27 Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii Meng, Xiangnan Yu, Yongbo Song, Tiefeng Yu, Yang Cui, Na Ma, Zhangtong Chen, Lijie Fan, Haiyan Front Plant Sci Plant Science Powdery mildew (PM) caused by Podosphaera xanthii poses a continuous threat to the performance and yield of the cucumber (Cucumis sativus L.). Control in the initial stages of infection is particularly important. Here, we studied the differential physiological and transcriptomic changes between PM-resistant strain B21-a-2-1-2 and PM-susceptible strain B21-a-2-2-2 at the early stage of P. xanthii attack. When challenged with P. xanthii, the tolerant line can postpone the formation of the pathogen primary germ. Comparative transcriptomic analysis suggested that DEGs related to the cell wall and to pathogen and hormone responses were similar enriched in both cucumber lines under P. xanthii infection. Notably, the number of DEGs triggered by P. xanthii in B21-a-2-1-2 was quintuple that in B21-a-2-2-2, revealing that the success of defense of resistant cucumber is due to rapidly mobilizing multiple responses. The unique responses detected were genes related to SA signaling, MAPK signaling, and Dof and WRKY transcription factors. Furthermore, 5 P. xanthii -inducible hub genes were identified, including GLPK, ILK1, EIN2, BCDHβ1, and RGGA, which are considered to be key candidate genes for disease control. This study combined multiple analytical approaches to capture potential molecular players and will provide key resources for developing cucumber cultivars resistant to pathogen stress. Frontiers Media S.A. 2022-05-12 /pmc/articles/PMC9134894/ /pubmed/35645993 http://dx.doi.org/10.3389/fpls.2022.872218 Text en Copyright © 2022 Meng, Yu, Song, Yu, Cui, Ma, Chen and Fan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Meng, Xiangnan
Yu, Yongbo
Song, Tiefeng
Yu, Yang
Cui, Na
Ma, Zhangtong
Chen, Lijie
Fan, Haiyan
Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii
title Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii
title_full Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii
title_fullStr Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii
title_full_unstemmed Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii
title_short Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii
title_sort transcriptome sequence analysis of the defense responses of resistant and susceptible cucumber strains to podosphaera xanthii
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134894/
https://www.ncbi.nlm.nih.gov/pubmed/35645993
http://dx.doi.org/10.3389/fpls.2022.872218
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