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Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds
Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135328/ https://www.ncbi.nlm.nih.gov/pubmed/35576189 http://dx.doi.org/10.1371/journal.pntd.0010431 |
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author | Nie, Lan-Bi Liang, Qin-Li Wang, Meng Du, Rui Zhang, Meng-Yuan Elsheikha, Hany M. Zhu, Xing-Quan |
author_facet | Nie, Lan-Bi Liang, Qin-Li Wang, Meng Du, Rui Zhang, Meng-Yuan Elsheikha, Hany M. Zhu, Xing-Quan |
author_sort | Nie, Lan-Bi |
collection | PubMed |
description | Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to understand the extent to which lysine malonylation contributes to the differences in the virulence and repertoire of virulence factors between T. gondii genotypes. In this study, we profiled lysine malonylation in T. gondii using quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immuno-affinity purification. This analysis was performed on three T. gondii strains with distinctive pathogenicity in mice, including RH strain (type I), PRU strain (type II), and VEG strain (type III). In total, 111 differentially malonylated proteins and 152 sites were upregulated, and 17 proteins and 17 sites were downregulated in RH strain versus PRU strain; 50 proteins and 59 sites were upregulated, 50 proteins and 53 sites were downregulated in RH strain versus VEG strain; and 72 proteins and 90 sites were upregulated, and 7 proteins and 8 sites were downregulated in VEG strain versus PRU strain. Differentially malonylated proteins were involved in key processes, such as those mediating the regulation of protein metabolism, stress response, glycolysis, and actin cytoskeleton. These results reveal an association between lysine malonylation and intra-species virulence differences in T. gondii and offer a new resource for elucidating the contribution of lysine malonylation to energy metabolism and virulence in T. gondii. |
format | Online Article Text |
id | pubmed-9135328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-91353282022-05-27 Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds Nie, Lan-Bi Liang, Qin-Li Wang, Meng Du, Rui Zhang, Meng-Yuan Elsheikha, Hany M. Zhu, Xing-Quan PLoS Negl Trop Dis Research Article Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to understand the extent to which lysine malonylation contributes to the differences in the virulence and repertoire of virulence factors between T. gondii genotypes. In this study, we profiled lysine malonylation in T. gondii using quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immuno-affinity purification. This analysis was performed on three T. gondii strains with distinctive pathogenicity in mice, including RH strain (type I), PRU strain (type II), and VEG strain (type III). In total, 111 differentially malonylated proteins and 152 sites were upregulated, and 17 proteins and 17 sites were downregulated in RH strain versus PRU strain; 50 proteins and 59 sites were upregulated, 50 proteins and 53 sites were downregulated in RH strain versus VEG strain; and 72 proteins and 90 sites were upregulated, and 7 proteins and 8 sites were downregulated in VEG strain versus PRU strain. Differentially malonylated proteins were involved in key processes, such as those mediating the regulation of protein metabolism, stress response, glycolysis, and actin cytoskeleton. These results reveal an association between lysine malonylation and intra-species virulence differences in T. gondii and offer a new resource for elucidating the contribution of lysine malonylation to energy metabolism and virulence in T. gondii. Public Library of Science 2022-05-16 /pmc/articles/PMC9135328/ /pubmed/35576189 http://dx.doi.org/10.1371/journal.pntd.0010431 Text en © 2022 Nie et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nie, Lan-Bi Liang, Qin-Li Wang, Meng Du, Rui Zhang, Meng-Yuan Elsheikha, Hany M. Zhu, Xing-Quan Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds |
title | Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds |
title_full | Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds |
title_fullStr | Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds |
title_full_unstemmed | Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds |
title_short | Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds |
title_sort | global profiling of protein lysine malonylation in toxoplasma gondii strains of different virulence and genetic backgrounds |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135328/ https://www.ncbi.nlm.nih.gov/pubmed/35576189 http://dx.doi.org/10.1371/journal.pntd.0010431 |
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