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Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds

Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to...

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Autores principales: Nie, Lan-Bi, Liang, Qin-Li, Wang, Meng, Du, Rui, Zhang, Meng-Yuan, Elsheikha, Hany M., Zhu, Xing-Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135328/
https://www.ncbi.nlm.nih.gov/pubmed/35576189
http://dx.doi.org/10.1371/journal.pntd.0010431
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author Nie, Lan-Bi
Liang, Qin-Li
Wang, Meng
Du, Rui
Zhang, Meng-Yuan
Elsheikha, Hany M.
Zhu, Xing-Quan
author_facet Nie, Lan-Bi
Liang, Qin-Li
Wang, Meng
Du, Rui
Zhang, Meng-Yuan
Elsheikha, Hany M.
Zhu, Xing-Quan
author_sort Nie, Lan-Bi
collection PubMed
description Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to understand the extent to which lysine malonylation contributes to the differences in the virulence and repertoire of virulence factors between T. gondii genotypes. In this study, we profiled lysine malonylation in T. gondii using quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immuno-affinity purification. This analysis was performed on three T. gondii strains with distinctive pathogenicity in mice, including RH strain (type I), PRU strain (type II), and VEG strain (type III). In total, 111 differentially malonylated proteins and 152 sites were upregulated, and 17 proteins and 17 sites were downregulated in RH strain versus PRU strain; 50 proteins and 59 sites were upregulated, 50 proteins and 53 sites were downregulated in RH strain versus VEG strain; and 72 proteins and 90 sites were upregulated, and 7 proteins and 8 sites were downregulated in VEG strain versus PRU strain. Differentially malonylated proteins were involved in key processes, such as those mediating the regulation of protein metabolism, stress response, glycolysis, and actin cytoskeleton. These results reveal an association between lysine malonylation and intra-species virulence differences in T. gondii and offer a new resource for elucidating the contribution of lysine malonylation to energy metabolism and virulence in T. gondii.
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spelling pubmed-91353282022-05-27 Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds Nie, Lan-Bi Liang, Qin-Li Wang, Meng Du, Rui Zhang, Meng-Yuan Elsheikha, Hany M. Zhu, Xing-Quan PLoS Negl Trop Dis Research Article Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to understand the extent to which lysine malonylation contributes to the differences in the virulence and repertoire of virulence factors between T. gondii genotypes. In this study, we profiled lysine malonylation in T. gondii using quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immuno-affinity purification. This analysis was performed on three T. gondii strains with distinctive pathogenicity in mice, including RH strain (type I), PRU strain (type II), and VEG strain (type III). In total, 111 differentially malonylated proteins and 152 sites were upregulated, and 17 proteins and 17 sites were downregulated in RH strain versus PRU strain; 50 proteins and 59 sites were upregulated, 50 proteins and 53 sites were downregulated in RH strain versus VEG strain; and 72 proteins and 90 sites were upregulated, and 7 proteins and 8 sites were downregulated in VEG strain versus PRU strain. Differentially malonylated proteins were involved in key processes, such as those mediating the regulation of protein metabolism, stress response, glycolysis, and actin cytoskeleton. These results reveal an association between lysine malonylation and intra-species virulence differences in T. gondii and offer a new resource for elucidating the contribution of lysine malonylation to energy metabolism and virulence in T. gondii. Public Library of Science 2022-05-16 /pmc/articles/PMC9135328/ /pubmed/35576189 http://dx.doi.org/10.1371/journal.pntd.0010431 Text en © 2022 Nie et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nie, Lan-Bi
Liang, Qin-Li
Wang, Meng
Du, Rui
Zhang, Meng-Yuan
Elsheikha, Hany M.
Zhu, Xing-Quan
Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds
title Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds
title_full Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds
title_fullStr Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds
title_full_unstemmed Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds
title_short Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds
title_sort global profiling of protein lysine malonylation in toxoplasma gondii strains of different virulence and genetic backgrounds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135328/
https://www.ncbi.nlm.nih.gov/pubmed/35576189
http://dx.doi.org/10.1371/journal.pntd.0010431
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