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Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes

Establishment of novel mosquito control technologies such as the use of genetically engineered insects typically involves phased testing to generate robust data-sets that support its safe and effective use as a vector control tool. In this study, we demonstrate the ability of the transgenic self-lim...

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Autores principales: Patil, Prabhakargouda B., Dasgupta, Shaibal Kumar, Gorman, Kevin, Pickl-Herk, Angela, Puinean, Mirel, McKemey, Andrew, Char, Bharat, Zehr, Usha B., Barwale, Shirish R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135344/
https://www.ncbi.nlm.nih.gov/pubmed/35576193
http://dx.doi.org/10.1371/journal.pntd.0010315
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author Patil, Prabhakargouda B.
Dasgupta, Shaibal Kumar
Gorman, Kevin
Pickl-Herk, Angela
Puinean, Mirel
McKemey, Andrew
Char, Bharat
Zehr, Usha B.
Barwale, Shirish R.
author_facet Patil, Prabhakargouda B.
Dasgupta, Shaibal Kumar
Gorman, Kevin
Pickl-Herk, Angela
Puinean, Mirel
McKemey, Andrew
Char, Bharat
Zehr, Usha B.
Barwale, Shirish R.
author_sort Patil, Prabhakargouda B.
collection PubMed
description Establishment of novel mosquito control technologies such as the use of genetically engineered insects typically involves phased testing to generate robust data-sets that support its safe and effective use as a vector control tool. In this study, we demonstrate the ability of the transgenic self-limiting OX513A Aedes aegypti strain to suppress a wild type Ae. aegypti population in an outdoor containment facility in India. OX513A is a genetically engineered Ae. aegypti strain with a repressible dominant self-limiting gene. When male adult OX513A mate with wild female adults, a single copy of the self-limiting gene is inherited by all the progeny, leading to death of >95% of progeny during larval/pupal development. A wild-type population of Ae. aegypti was established and stabilized during a 14 week period in five paired field cage units, each consisting of control and treatment cages, followed by weekly releases of OX513A male adults to suppress the target population. The successive introductions of OX513A male adults led to a consistent decline in wild type numbers eventually resulting in the elimination of Ae. aegypti from all treated cages within 10 to 15 weeks of release. This study demonstrates that Ae. aegypti elimination may be a realistic and achievable target in relatively isolated environments.
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spelling pubmed-91353442022-05-27 Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes Patil, Prabhakargouda B. Dasgupta, Shaibal Kumar Gorman, Kevin Pickl-Herk, Angela Puinean, Mirel McKemey, Andrew Char, Bharat Zehr, Usha B. Barwale, Shirish R. PLoS Negl Trop Dis Research Article Establishment of novel mosquito control technologies such as the use of genetically engineered insects typically involves phased testing to generate robust data-sets that support its safe and effective use as a vector control tool. In this study, we demonstrate the ability of the transgenic self-limiting OX513A Aedes aegypti strain to suppress a wild type Ae. aegypti population in an outdoor containment facility in India. OX513A is a genetically engineered Ae. aegypti strain with a repressible dominant self-limiting gene. When male adult OX513A mate with wild female adults, a single copy of the self-limiting gene is inherited by all the progeny, leading to death of >95% of progeny during larval/pupal development. A wild-type population of Ae. aegypti was established and stabilized during a 14 week period in five paired field cage units, each consisting of control and treatment cages, followed by weekly releases of OX513A male adults to suppress the target population. The successive introductions of OX513A male adults led to a consistent decline in wild type numbers eventually resulting in the elimination of Ae. aegypti from all treated cages within 10 to 15 weeks of release. This study demonstrates that Ae. aegypti elimination may be a realistic and achievable target in relatively isolated environments. Public Library of Science 2022-05-16 /pmc/articles/PMC9135344/ /pubmed/35576193 http://dx.doi.org/10.1371/journal.pntd.0010315 Text en © 2022 Patil et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Patil, Prabhakargouda B.
Dasgupta, Shaibal Kumar
Gorman, Kevin
Pickl-Herk, Angela
Puinean, Mirel
McKemey, Andrew
Char, Bharat
Zehr, Usha B.
Barwale, Shirish R.
Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes
title Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes
title_full Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes
title_fullStr Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes
title_full_unstemmed Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes
title_short Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes
title_sort elimination of a closed population of the yellow fever mosquito, aedes aegypti, through releases of self-limiting male mosquitoes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135344/
https://www.ncbi.nlm.nih.gov/pubmed/35576193
http://dx.doi.org/10.1371/journal.pntd.0010315
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