Cargando…
Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes
Establishment of novel mosquito control technologies such as the use of genetically engineered insects typically involves phased testing to generate robust data-sets that support its safe and effective use as a vector control tool. In this study, we demonstrate the ability of the transgenic self-lim...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135344/ https://www.ncbi.nlm.nih.gov/pubmed/35576193 http://dx.doi.org/10.1371/journal.pntd.0010315 |
_version_ | 1784713943227301888 |
---|---|
author | Patil, Prabhakargouda B. Dasgupta, Shaibal Kumar Gorman, Kevin Pickl-Herk, Angela Puinean, Mirel McKemey, Andrew Char, Bharat Zehr, Usha B. Barwale, Shirish R. |
author_facet | Patil, Prabhakargouda B. Dasgupta, Shaibal Kumar Gorman, Kevin Pickl-Herk, Angela Puinean, Mirel McKemey, Andrew Char, Bharat Zehr, Usha B. Barwale, Shirish R. |
author_sort | Patil, Prabhakargouda B. |
collection | PubMed |
description | Establishment of novel mosquito control technologies such as the use of genetically engineered insects typically involves phased testing to generate robust data-sets that support its safe and effective use as a vector control tool. In this study, we demonstrate the ability of the transgenic self-limiting OX513A Aedes aegypti strain to suppress a wild type Ae. aegypti population in an outdoor containment facility in India. OX513A is a genetically engineered Ae. aegypti strain with a repressible dominant self-limiting gene. When male adult OX513A mate with wild female adults, a single copy of the self-limiting gene is inherited by all the progeny, leading to death of >95% of progeny during larval/pupal development. A wild-type population of Ae. aegypti was established and stabilized during a 14 week period in five paired field cage units, each consisting of control and treatment cages, followed by weekly releases of OX513A male adults to suppress the target population. The successive introductions of OX513A male adults led to a consistent decline in wild type numbers eventually resulting in the elimination of Ae. aegypti from all treated cages within 10 to 15 weeks of release. This study demonstrates that Ae. aegypti elimination may be a realistic and achievable target in relatively isolated environments. |
format | Online Article Text |
id | pubmed-9135344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-91353442022-05-27 Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes Patil, Prabhakargouda B. Dasgupta, Shaibal Kumar Gorman, Kevin Pickl-Herk, Angela Puinean, Mirel McKemey, Andrew Char, Bharat Zehr, Usha B. Barwale, Shirish R. PLoS Negl Trop Dis Research Article Establishment of novel mosquito control technologies such as the use of genetically engineered insects typically involves phased testing to generate robust data-sets that support its safe and effective use as a vector control tool. In this study, we demonstrate the ability of the transgenic self-limiting OX513A Aedes aegypti strain to suppress a wild type Ae. aegypti population in an outdoor containment facility in India. OX513A is a genetically engineered Ae. aegypti strain with a repressible dominant self-limiting gene. When male adult OX513A mate with wild female adults, a single copy of the self-limiting gene is inherited by all the progeny, leading to death of >95% of progeny during larval/pupal development. A wild-type population of Ae. aegypti was established and stabilized during a 14 week period in five paired field cage units, each consisting of control and treatment cages, followed by weekly releases of OX513A male adults to suppress the target population. The successive introductions of OX513A male adults led to a consistent decline in wild type numbers eventually resulting in the elimination of Ae. aegypti from all treated cages within 10 to 15 weeks of release. This study demonstrates that Ae. aegypti elimination may be a realistic and achievable target in relatively isolated environments. Public Library of Science 2022-05-16 /pmc/articles/PMC9135344/ /pubmed/35576193 http://dx.doi.org/10.1371/journal.pntd.0010315 Text en © 2022 Patil et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Patil, Prabhakargouda B. Dasgupta, Shaibal Kumar Gorman, Kevin Pickl-Herk, Angela Puinean, Mirel McKemey, Andrew Char, Bharat Zehr, Usha B. Barwale, Shirish R. Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes |
title | Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes |
title_full | Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes |
title_fullStr | Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes |
title_full_unstemmed | Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes |
title_short | Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes |
title_sort | elimination of a closed population of the yellow fever mosquito, aedes aegypti, through releases of self-limiting male mosquitoes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135344/ https://www.ncbi.nlm.nih.gov/pubmed/35576193 http://dx.doi.org/10.1371/journal.pntd.0010315 |
work_keys_str_mv | AT patilprabhakargoudab eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes AT dasguptashaibalkumar eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes AT gormankevin eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes AT picklherkangela eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes AT puineanmirel eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes AT mckemeyandrew eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes AT charbharat eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes AT zehrushab eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes AT barwaleshirishr eliminationofaclosedpopulationoftheyellowfevermosquitoaedesaegyptithroughreleasesofselflimitingmalemosquitoes |