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Levetiracetam Attenuates the Spinal Cord Injury Induced by Acute Trauma via Suppressing the Expression of Perforin

The spinal cord injury (SCI) is one of the major reasons causing the motor dysfunctions of the patients. At present, few therapeutic strategies can effectively improve the symptom of SCI. Levetiracetam has been confirmed to alleviate the injury of nerve cells, while its functions in SCI remains uncl...

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Autores principales: Xia, Hong, Huang, Jiaqiang, Liu, Jian, Zhang, Hua, Chen, Dawei, Zhang, Wen, Zhou, Xianghong, Wei, Zhouyang, Liu, Zhaoxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135510/
https://www.ncbi.nlm.nih.gov/pubmed/35633929
http://dx.doi.org/10.1155/2022/7218666
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author Xia, Hong
Huang, Jiaqiang
Liu, Jian
Zhang, Hua
Chen, Dawei
Zhang, Wen
Zhou, Xianghong
Wei, Zhouyang
Liu, Zhaoxiang
author_facet Xia, Hong
Huang, Jiaqiang
Liu, Jian
Zhang, Hua
Chen, Dawei
Zhang, Wen
Zhou, Xianghong
Wei, Zhouyang
Liu, Zhaoxiang
author_sort Xia, Hong
collection PubMed
description The spinal cord injury (SCI) is one of the major reasons causing the motor dysfunctions of the patients. At present, few therapeutic strategies can effectively improve the symptom of SCI. Levetiracetam has been confirmed to alleviate the injury of nerve cells, while its functions in SCI remains unclear. In this study, C57BL/6J mice were used to establish SCI models to observe the effect of levetiracetam on SCI. The mice were fed with 180 mg/kg levetiracetam when suffering from SCI, and Basso mouse score (BMS) and CatWalk-assisted gait analysis were used to observe the motor functions of the mice. Nissl staining and TUNEL staining were used to observe the injury of nerve cells. The abundance of inflammatory factors was measured by ELISA. The permeability of blood-spinal cord barrier (BSCB) in mice was detected with macrophage infiltration analysis. Moreover, the abundance of perforin in the tissues was detected by western blot. The results showed that the SCI mice treated with levetiracetam exhibited lighter motor dysfunction compared with the mice treated with saline. Levetiracetam can effectively reduce the inflammatory reactions and alleviate apoptosis of the nerve cells. Moreover, levetiracetam remarkably decreased the BSCB permeability of SCI mice. Besides, it was also found that levetiracetam can significantly inhibit the expression of perforin. In conclusion, this study suggests that levetiracetam can attenuate the injury of BSCB to block the progression of SCI via suppressing the expression of perforin.
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spelling pubmed-91355102022-05-27 Levetiracetam Attenuates the Spinal Cord Injury Induced by Acute Trauma via Suppressing the Expression of Perforin Xia, Hong Huang, Jiaqiang Liu, Jian Zhang, Hua Chen, Dawei Zhang, Wen Zhou, Xianghong Wei, Zhouyang Liu, Zhaoxiang Comput Math Methods Med Research Article The spinal cord injury (SCI) is one of the major reasons causing the motor dysfunctions of the patients. At present, few therapeutic strategies can effectively improve the symptom of SCI. Levetiracetam has been confirmed to alleviate the injury of nerve cells, while its functions in SCI remains unclear. In this study, C57BL/6J mice were used to establish SCI models to observe the effect of levetiracetam on SCI. The mice were fed with 180 mg/kg levetiracetam when suffering from SCI, and Basso mouse score (BMS) and CatWalk-assisted gait analysis were used to observe the motor functions of the mice. Nissl staining and TUNEL staining were used to observe the injury of nerve cells. The abundance of inflammatory factors was measured by ELISA. The permeability of blood-spinal cord barrier (BSCB) in mice was detected with macrophage infiltration analysis. Moreover, the abundance of perforin in the tissues was detected by western blot. The results showed that the SCI mice treated with levetiracetam exhibited lighter motor dysfunction compared with the mice treated with saline. Levetiracetam can effectively reduce the inflammatory reactions and alleviate apoptosis of the nerve cells. Moreover, levetiracetam remarkably decreased the BSCB permeability of SCI mice. Besides, it was also found that levetiracetam can significantly inhibit the expression of perforin. In conclusion, this study suggests that levetiracetam can attenuate the injury of BSCB to block the progression of SCI via suppressing the expression of perforin. Hindawi 2022-05-19 /pmc/articles/PMC9135510/ /pubmed/35633929 http://dx.doi.org/10.1155/2022/7218666 Text en Copyright © 2022 Hong Xia et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xia, Hong
Huang, Jiaqiang
Liu, Jian
Zhang, Hua
Chen, Dawei
Zhang, Wen
Zhou, Xianghong
Wei, Zhouyang
Liu, Zhaoxiang
Levetiracetam Attenuates the Spinal Cord Injury Induced by Acute Trauma via Suppressing the Expression of Perforin
title Levetiracetam Attenuates the Spinal Cord Injury Induced by Acute Trauma via Suppressing the Expression of Perforin
title_full Levetiracetam Attenuates the Spinal Cord Injury Induced by Acute Trauma via Suppressing the Expression of Perforin
title_fullStr Levetiracetam Attenuates the Spinal Cord Injury Induced by Acute Trauma via Suppressing the Expression of Perforin
title_full_unstemmed Levetiracetam Attenuates the Spinal Cord Injury Induced by Acute Trauma via Suppressing the Expression of Perforin
title_short Levetiracetam Attenuates the Spinal Cord Injury Induced by Acute Trauma via Suppressing the Expression of Perforin
title_sort levetiracetam attenuates the spinal cord injury induced by acute trauma via suppressing the expression of perforin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135510/
https://www.ncbi.nlm.nih.gov/pubmed/35633929
http://dx.doi.org/10.1155/2022/7218666
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