The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine

The genetic capacity to synthesize the biofilm matrix exopolysaccharide Pel is widespread among Gram-negative and Gram-positive bacteria. However, its exact chemical structure has been challenging to determine. Using a Pseudomonas aeruginosa strain engineered to overproduce Pel, improvements to the...

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Autores principales: Le Mauff, François, Razvi, Erum, Reichhardt, Courtney, Sivarajah, Piyanka, Parsek, Matthew R., Howell, P. Lynne, Sheppard, Donald C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135694/
https://www.ncbi.nlm.nih.gov/pubmed/35618750
http://dx.doi.org/10.1038/s42003-022-03453-2
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author Le Mauff, François
Razvi, Erum
Reichhardt, Courtney
Sivarajah, Piyanka
Parsek, Matthew R.
Howell, P. Lynne
Sheppard, Donald C.
author_facet Le Mauff, François
Razvi, Erum
Reichhardt, Courtney
Sivarajah, Piyanka
Parsek, Matthew R.
Howell, P. Lynne
Sheppard, Donald C.
author_sort Le Mauff, François
collection PubMed
description The genetic capacity to synthesize the biofilm matrix exopolysaccharide Pel is widespread among Gram-negative and Gram-positive bacteria. However, its exact chemical structure has been challenging to determine. Using a Pseudomonas aeruginosa strain engineered to overproduce Pel, improvements to the isolation procedure, and selective hydrolysis with the glycoside hydrolase PelA(h), we demonstrate that Pel is a partially de-N-acetylated linear polymer of α-1,4-N-acetylgalactosamine comprised predominantly of dimeric repeats of galactosamine and N-acetylgalactosamine.
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spelling pubmed-91356942022-05-28 The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine Le Mauff, François Razvi, Erum Reichhardt, Courtney Sivarajah, Piyanka Parsek, Matthew R. Howell, P. Lynne Sheppard, Donald C. Commun Biol Article The genetic capacity to synthesize the biofilm matrix exopolysaccharide Pel is widespread among Gram-negative and Gram-positive bacteria. However, its exact chemical structure has been challenging to determine. Using a Pseudomonas aeruginosa strain engineered to overproduce Pel, improvements to the isolation procedure, and selective hydrolysis with the glycoside hydrolase PelA(h), we demonstrate that Pel is a partially de-N-acetylated linear polymer of α-1,4-N-acetylgalactosamine comprised predominantly of dimeric repeats of galactosamine and N-acetylgalactosamine. Nature Publishing Group UK 2022-05-26 /pmc/articles/PMC9135694/ /pubmed/35618750 http://dx.doi.org/10.1038/s42003-022-03453-2 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Le Mauff, François
Razvi, Erum
Reichhardt, Courtney
Sivarajah, Piyanka
Parsek, Matthew R.
Howell, P. Lynne
Sheppard, Donald C.
The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine
title The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine
title_full The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine
title_fullStr The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine
title_full_unstemmed The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine
title_short The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine
title_sort pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and n-acetylgalactosamine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135694/
https://www.ncbi.nlm.nih.gov/pubmed/35618750
http://dx.doi.org/10.1038/s42003-022-03453-2
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