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Epigenetic clock and methylation studies in vervet monkeys

DNA methylation-based biomarkers of aging have been developed for many mammals but not yet for the vervet monkey (Chlorocebus sabaeus), which is a valuable non-human primate model for biomedical studies. We generated novel DNA methylation data from vervet cerebral cortex, blood, and liver using high...

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Autores principales: Jasinska, Anna J., Haghani, Amin, Zoller, Joseph A., Li, Caesar Z., Arneson, Adriana, Ernst, Jason, Kavanagh, Kylie, Jorgensen, Matthew J., Mattison, Julie A., Wojta, Kevin, Choi, Oi-Wa, DeYoung, Joseph, Li, Xinmin, Rao, Andrew W., Coppola, Giovanni, Freimer, Nelson B., Woods, Roger P., Horvath, Steve
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135907/
https://www.ncbi.nlm.nih.gov/pubmed/34591235
http://dx.doi.org/10.1007/s11357-021-00466-3
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author Jasinska, Anna J.
Haghani, Amin
Zoller, Joseph A.
Li, Caesar Z.
Arneson, Adriana
Ernst, Jason
Kavanagh, Kylie
Jorgensen, Matthew J.
Mattison, Julie A.
Wojta, Kevin
Choi, Oi-Wa
DeYoung, Joseph
Li, Xinmin
Rao, Andrew W.
Coppola, Giovanni
Freimer, Nelson B.
Woods, Roger P.
Horvath, Steve
author_facet Jasinska, Anna J.
Haghani, Amin
Zoller, Joseph A.
Li, Caesar Z.
Arneson, Adriana
Ernst, Jason
Kavanagh, Kylie
Jorgensen, Matthew J.
Mattison, Julie A.
Wojta, Kevin
Choi, Oi-Wa
DeYoung, Joseph
Li, Xinmin
Rao, Andrew W.
Coppola, Giovanni
Freimer, Nelson B.
Woods, Roger P.
Horvath, Steve
author_sort Jasinska, Anna J.
collection PubMed
description DNA methylation-based biomarkers of aging have been developed for many mammals but not yet for the vervet monkey (Chlorocebus sabaeus), which is a valuable non-human primate model for biomedical studies. We generated novel DNA methylation data from vervet cerebral cortex, blood, and liver using highly conserved mammalian CpGs represented on a custom array (HorvathMammalMethylChip40). We present six DNA methylation-based estimators of age: vervet multi-tissue epigenetic clock and tissue-specific clocks for brain cortex, blood, and liver. In addition, we developed two dual species clocks (human-vervet clocks) for measuring chronological age and relative age, respectively. Relative age was defined as ratio of chronological age to maximum lifespan to address the species differences in maximum lifespan. The high accuracy of the human-vervet clocks demonstrates that epigenetic aging processes are evolutionary conserved in primates. When applying these vervet clocks to tissue samples from another primate species, rhesus macaque, we observed high age correlations but strong offsets. We characterized CpGs that correlate significantly with age in the vervet. CpG probes that gain methylation with age across tissues were located near the targets of Polycomb proteins SUZ12 and EED and genes possessing the trimethylated H3K27 mark in their promoters. The epigenetic clocks are expected to be useful for anti-aging studies in vervets. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11357-021-00466-3.
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spelling pubmed-91359072022-05-28 Epigenetic clock and methylation studies in vervet monkeys Jasinska, Anna J. Haghani, Amin Zoller, Joseph A. Li, Caesar Z. Arneson, Adriana Ernst, Jason Kavanagh, Kylie Jorgensen, Matthew J. Mattison, Julie A. Wojta, Kevin Choi, Oi-Wa DeYoung, Joseph Li, Xinmin Rao, Andrew W. Coppola, Giovanni Freimer, Nelson B. Woods, Roger P. Horvath, Steve GeroScience Original Article DNA methylation-based biomarkers of aging have been developed for many mammals but not yet for the vervet monkey (Chlorocebus sabaeus), which is a valuable non-human primate model for biomedical studies. We generated novel DNA methylation data from vervet cerebral cortex, blood, and liver using highly conserved mammalian CpGs represented on a custom array (HorvathMammalMethylChip40). We present six DNA methylation-based estimators of age: vervet multi-tissue epigenetic clock and tissue-specific clocks for brain cortex, blood, and liver. In addition, we developed two dual species clocks (human-vervet clocks) for measuring chronological age and relative age, respectively. Relative age was defined as ratio of chronological age to maximum lifespan to address the species differences in maximum lifespan. The high accuracy of the human-vervet clocks demonstrates that epigenetic aging processes are evolutionary conserved in primates. When applying these vervet clocks to tissue samples from another primate species, rhesus macaque, we observed high age correlations but strong offsets. We characterized CpGs that correlate significantly with age in the vervet. CpG probes that gain methylation with age across tissues were located near the targets of Polycomb proteins SUZ12 and EED and genes possessing the trimethylated H3K27 mark in their promoters. The epigenetic clocks are expected to be useful for anti-aging studies in vervets. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11357-021-00466-3. Springer International Publishing 2021-09-30 /pmc/articles/PMC9135907/ /pubmed/34591235 http://dx.doi.org/10.1007/s11357-021-00466-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Jasinska, Anna J.
Haghani, Amin
Zoller, Joseph A.
Li, Caesar Z.
Arneson, Adriana
Ernst, Jason
Kavanagh, Kylie
Jorgensen, Matthew J.
Mattison, Julie A.
Wojta, Kevin
Choi, Oi-Wa
DeYoung, Joseph
Li, Xinmin
Rao, Andrew W.
Coppola, Giovanni
Freimer, Nelson B.
Woods, Roger P.
Horvath, Steve
Epigenetic clock and methylation studies in vervet monkeys
title Epigenetic clock and methylation studies in vervet monkeys
title_full Epigenetic clock and methylation studies in vervet monkeys
title_fullStr Epigenetic clock and methylation studies in vervet monkeys
title_full_unstemmed Epigenetic clock and methylation studies in vervet monkeys
title_short Epigenetic clock and methylation studies in vervet monkeys
title_sort epigenetic clock and methylation studies in vervet monkeys
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135907/
https://www.ncbi.nlm.nih.gov/pubmed/34591235
http://dx.doi.org/10.1007/s11357-021-00466-3
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