Cargando…

High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China

BACKGROUND: All Chinese blood centers have implemented mini pool (MP) HBV nucleic acid testing (NAT) together with HBsAg ELISA in routine donor screening since 2015. The prevalence of occult hepatitis B virus infection (OBI) in donors from different regions varies, and the molecular characterization...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Xianlin, Liu, Lihua, Chen, Lina, Nie, Xianghui, Huang, Lu, Ye, Denghuang, Zeng, Jinfeng, Li, Tong, Li, Bin, Xu, Min, Chen, Limin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136029/
https://www.ncbi.nlm.nih.gov/pubmed/35634299
http://dx.doi.org/10.3389/fimmu.2022.754383
_version_ 1784714085781209088
author Ye, Xianlin
Liu, Lihua
Chen, Lina
Nie, Xianghui
Huang, Lu
Ye, Denghuang
Zeng, Jinfeng
Li, Tong
Li, Bin
Xu, Min
Chen, Limin
author_facet Ye, Xianlin
Liu, Lihua
Chen, Lina
Nie, Xianghui
Huang, Lu
Ye, Denghuang
Zeng, Jinfeng
Li, Tong
Li, Bin
Xu, Min
Chen, Limin
author_sort Ye, Xianlin
collection PubMed
description BACKGROUND: All Chinese blood centers have implemented mini pool (MP) HBV nucleic acid testing (NAT) together with HBsAg ELISA in routine donor screening since 2015. The prevalence of occult hepatitis B virus infection (OBI) in donors from different regions varies, and the molecular characterization of the HBV DNA and clinical outcomes of these OBIs remain largely unexplored. METHODS: Blood donations from Heyuan city in Southern China were screened by HBsAg ELISA and HBV MP8 NAT. Donations with HBsAg-/HBV DNA+ were collected for this study. Molecular characterizations of HBV DNAs were further analyzed by various DNA amplification assays including quantitative PCR (qPCR) and nested PCR, amplifying the basic core and pre-core promoter regions (BCP/PC). The HBsAg (S) region from HBV DNA was isolated by high-volume nucleic acid extraction. Notable mutations were identified by comparison to the HBV reference sequences. The clinical outcomes of the donors with OBIs were further followed for nearly 3 years. RESULTS: Seventy OBIs from 44,592 donations (0.15%) that we identified and reported previously were enrolled for this current study. HBV sequences were obtained from 44/70 OBIs, and genotyping analysis showed that 42/44 (95.2%) OBIs were genotype B, and 2/44 (4.8%) were genotype C. Interestingly, mutation analysis revealed that various mutations including M133L/T, F134L, P142L, V168A, R169H, S174N, L175S, and V177A of HBV DNA affecting HBsAg detection were observed in genotype B OBIs. Two notable mutations, T47K and L53S, were identified in genotype C OBIs. Follow-up studies showed that 3/31 (9.7%) OBIs converted to HBsAg+ as chronic infections while 1/31 (3.2%) HBV DNA was undetectable (classified as recovery) and 27/31 (87.1%) remained as OBIs. CONCLUSION: Various notable mutations affecting HBsAg detection were observed in blood donors with OBIs in Heyuan city of Southern China. Follow-up studies showed that most OBIs remained as OBIs with fluctuating or low viral loads. Higher sensitive HBV ID NAT is recommended for donor screening to further reduce the transmission risk of OBIs.
format Online
Article
Text
id pubmed-9136029
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91360292022-05-28 High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China Ye, Xianlin Liu, Lihua Chen, Lina Nie, Xianghui Huang, Lu Ye, Denghuang Zeng, Jinfeng Li, Tong Li, Bin Xu, Min Chen, Limin Front Immunol Immunology BACKGROUND: All Chinese blood centers have implemented mini pool (MP) HBV nucleic acid testing (NAT) together with HBsAg ELISA in routine donor screening since 2015. The prevalence of occult hepatitis B virus infection (OBI) in donors from different regions varies, and the molecular characterization of the HBV DNA and clinical outcomes of these OBIs remain largely unexplored. METHODS: Blood donations from Heyuan city in Southern China were screened by HBsAg ELISA and HBV MP8 NAT. Donations with HBsAg-/HBV DNA+ were collected for this study. Molecular characterizations of HBV DNAs were further analyzed by various DNA amplification assays including quantitative PCR (qPCR) and nested PCR, amplifying the basic core and pre-core promoter regions (BCP/PC). The HBsAg (S) region from HBV DNA was isolated by high-volume nucleic acid extraction. Notable mutations were identified by comparison to the HBV reference sequences. The clinical outcomes of the donors with OBIs were further followed for nearly 3 years. RESULTS: Seventy OBIs from 44,592 donations (0.15%) that we identified and reported previously were enrolled for this current study. HBV sequences were obtained from 44/70 OBIs, and genotyping analysis showed that 42/44 (95.2%) OBIs were genotype B, and 2/44 (4.8%) were genotype C. Interestingly, mutation analysis revealed that various mutations including M133L/T, F134L, P142L, V168A, R169H, S174N, L175S, and V177A of HBV DNA affecting HBsAg detection were observed in genotype B OBIs. Two notable mutations, T47K and L53S, were identified in genotype C OBIs. Follow-up studies showed that 3/31 (9.7%) OBIs converted to HBsAg+ as chronic infections while 1/31 (3.2%) HBV DNA was undetectable (classified as recovery) and 27/31 (87.1%) remained as OBIs. CONCLUSION: Various notable mutations affecting HBsAg detection were observed in blood donors with OBIs in Heyuan city of Southern China. Follow-up studies showed that most OBIs remained as OBIs with fluctuating or low viral loads. Higher sensitive HBV ID NAT is recommended for donor screening to further reduce the transmission risk of OBIs. Frontiers Media S.A. 2022-05-13 /pmc/articles/PMC9136029/ /pubmed/35634299 http://dx.doi.org/10.3389/fimmu.2022.754383 Text en Copyright © 2022 Ye, Liu, Chen, Nie, Huang, Ye, Zeng, Li, Li, Xu and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Ye, Xianlin
Liu, Lihua
Chen, Lina
Nie, Xianghui
Huang, Lu
Ye, Denghuang
Zeng, Jinfeng
Li, Tong
Li, Bin
Xu, Min
Chen, Limin
High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China
title High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China
title_full High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China
title_fullStr High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China
title_full_unstemmed High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China
title_short High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China
title_sort high-frequency notable hbv mutations identified in blood donors with occult hepatitis b infection from heyuan city of southern china
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136029/
https://www.ncbi.nlm.nih.gov/pubmed/35634299
http://dx.doi.org/10.3389/fimmu.2022.754383
work_keys_str_mv AT yexianlin highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT liulihua highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT chenlina highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT niexianghui highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT huanglu highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT yedenghuang highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT zengjinfeng highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT litong highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT libin highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT xumin highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina
AT chenlimin highfrequencynotablehbvmutationsidentifiedinblooddonorswithocculthepatitisbinfectionfromheyuancityofsouthernchina