Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease

In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzhei...

Descripción completa

Detalles Bibliográficos
Autores principales: Ulian-Benitez, Suzana, Hjelmstad, Abigail Strausbaugh, Barbosa, Guilherme Oliveira, Haghighi, Pejmun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136343/
https://www.ncbi.nlm.nih.gov/pubmed/35634356
http://dx.doi.org/10.1016/j.xpro.2022.101377
Descripción
Sumario:In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzheimer disease. We describe eosin staining in the whole-brain mount, followed by confocal microscopy and image analysis with an open source Fiji plugin. This protocol can be applied to visualize different modules in the fly brain.

Ejemplares similares