Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease

In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzhei...

Descripción completa

Detalles Bibliográficos
Autores principales: Ulian-Benitez, Suzana, Hjelmstad, Abigail Strausbaugh, Barbosa, Guilherme Oliveira, Haghighi, Pejmun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136343/
https://www.ncbi.nlm.nih.gov/pubmed/35634356
http://dx.doi.org/10.1016/j.xpro.2022.101377
_version_ 1784714158926725120
author Ulian-Benitez, Suzana
Hjelmstad, Abigail Strausbaugh
Barbosa, Guilherme Oliveira
Haghighi, Pejmun
author_facet Ulian-Benitez, Suzana
Hjelmstad, Abigail Strausbaugh
Barbosa, Guilherme Oliveira
Haghighi, Pejmun
author_sort Ulian-Benitez, Suzana
collection PubMed
description In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzheimer disease. We describe eosin staining in the whole-brain mount, followed by confocal microscopy and image analysis with an open source Fiji plugin. This protocol can be applied to visualize different modules in the fly brain.
format Online
Article
Text
id pubmed-9136343
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-91363432022-05-28 Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease Ulian-Benitez, Suzana Hjelmstad, Abigail Strausbaugh Barbosa, Guilherme Oliveira Haghighi, Pejmun STAR Protoc Protocol In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzheimer disease. We describe eosin staining in the whole-brain mount, followed by confocal microscopy and image analysis with an open source Fiji plugin. This protocol can be applied to visualize different modules in the fly brain. Elsevier 2022-05-25 /pmc/articles/PMC9136343/ /pubmed/35634356 http://dx.doi.org/10.1016/j.xpro.2022.101377 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ulian-Benitez, Suzana
Hjelmstad, Abigail Strausbaugh
Barbosa, Guilherme Oliveira
Haghighi, Pejmun
Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease
title Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease
title_full Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease
title_fullStr Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease
title_full_unstemmed Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease
title_short Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease
title_sort eosin whole-brain mount staining to analyze neurodegeneration in a fly model of alzheimer’s disease
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136343/
https://www.ncbi.nlm.nih.gov/pubmed/35634356
http://dx.doi.org/10.1016/j.xpro.2022.101377
work_keys_str_mv AT ulianbenitezsuzana eosinwholebrainmountstainingtoanalyzeneurodegenerationinaflymodelofalzheimersdisease
AT hjelmstadabigailstrausbaugh eosinwholebrainmountstainingtoanalyzeneurodegenerationinaflymodelofalzheimersdisease
AT barbosaguilhermeoliveira eosinwholebrainmountstainingtoanalyzeneurodegenerationinaflymodelofalzheimersdisease
AT haghighipejmun eosinwholebrainmountstainingtoanalyzeneurodegenerationinaflymodelofalzheimersdisease

Ejemplares similares