A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System

OBJECTIVES: CRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens remains unclear. METHODS: A combined rational de...

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Detalles Bibliográficos
Autores principales: Bai, Xiaopeng, Gao, Panqi, Qian, Keli, Yang, Jiandong, Deng, Haijun, Fu, Tiwei, Hu, Yuan, Han, Miaomiao, Zheng, Huizhi, Cao, Xiaoxia, Liu, Yuliang, Lu, Yaoqin, Huang, Ailong, Long, Quanxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136396/
https://www.ncbi.nlm.nih.gov/pubmed/35633684
http://dx.doi.org/10.3389/fmicb.2022.847373

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