GFI1 regulates chromatin state essential in human endothelial‐to‐haematopoietic transition

OBJECTIVES: During embryonic haematopoiesis, haematopoietic stem/progenitor cells (HSPCs) develop from hemogenic endothelial cells (HECs) though endothelial to haematopoietic transition (EHT). However, little is known about how EHT is regulated in human. Here, we report that GFI1 plays an essential...

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Detalles Bibliográficos
Autores principales: Kang, Baoqiang, Zhang, Tian, Huang, Ke, Wang, Tianyu, Li, Yuhang, Mai, Yuchan, Li, Jinbing, Dang, Shiying, Zhang, Zhishuai, Huang, Wenhao, Wang, Junwei, Gao, Minghui, Wang, Yi, Pan, Guangjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136496/
https://www.ncbi.nlm.nih.gov/pubmed/35504619
http://dx.doi.org/10.1111/cpr.13244
Descripción
Sumario:OBJECTIVES: During embryonic haematopoiesis, haematopoietic stem/progenitor cells (HSPCs) develop from hemogenic endothelial cells (HECs) though endothelial to haematopoietic transition (EHT). However, little is known about how EHT is regulated in human. Here, we report that GFI1 plays an essential role in enabling normal EHT during haematopoietic differentiation of human embryonic stem cells (hESCs). RESULTS: GFI1 deletion in hESCs leads to a complete EHT defect due to a closed chromatin state of hematopoietic genes in HECs. Mechanically, directly regulates important signaling pathways essential for the EHT such as PI3K signaling.etc. CONCLUTIONS: Together, our findings reveal an essential role of GFI1 mediated epigenetic mechanism underlying human EHT during hematopoiesis.

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