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Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages

CRISPR-Cas9 screening libraries have arisen as a powerful tool to identify protein-coding (pc) and non-coding genes playing a role along different processes. In particular, the usage of a nuclease active Cas9 coupled to a single gRNA has proven to efficiently impair the expression of pc-genes by gen...

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Detalles Bibliográficos
Autores principales:	Arnan, Carme, Ullrich, Sebastian, Pulido-Quetglas, Carlos, Nurtdinov, Ramil, Esteban, Alexandre, Blanco-Fernandez, Joan, Aparicio-Prat, Estel, Johnson, Rory, Pérez-Lluch, Sílvia, Guigó, Roderic
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137126/ https://www.ncbi.nlm.nih.gov/pubmed/35619054 http://dx.doi.org/10.1186/s12864-022-08612-7

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