Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis

Background: The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment. Methods: Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selec...

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Autores principales: Zboromyrska, Yuliya, Rico, Verónica, Pitart, Cristina, Fernández-Pittol, Mariana José, Soriano, Álex, Bosch, Jordi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137483/
https://www.ncbi.nlm.nih.gov/pubmed/35625226
http://dx.doi.org/10.3390/antibiotics11050582
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author Zboromyrska, Yuliya
Rico, Verónica
Pitart, Cristina
Fernández-Pittol, Mariana José
Soriano, Álex
Bosch, Jordi
author_facet Zboromyrska, Yuliya
Rico, Verónica
Pitart, Cristina
Fernández-Pittol, Mariana José
Soriano, Álex
Bosch, Jordi
author_sort Zboromyrska, Yuliya
collection PubMed
description Background: The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment. Methods: Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed. Results: The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases. Conclusion: the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool.
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spelling pubmed-91374832022-05-28 Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis Zboromyrska, Yuliya Rico, Verónica Pitart, Cristina Fernández-Pittol, Mariana José Soriano, Álex Bosch, Jordi Antibiotics (Basel) Communication Background: The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment. Methods: Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed. Results: The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases. Conclusion: the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool. MDPI 2022-04-26 /pmc/articles/PMC9137483/ /pubmed/35625226 http://dx.doi.org/10.3390/antibiotics11050582 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Zboromyrska, Yuliya
Rico, Verónica
Pitart, Cristina
Fernández-Pittol, Mariana José
Soriano, Álex
Bosch, Jordi
Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
title Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
title_full Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
title_fullStr Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
title_full_unstemmed Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
title_short Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
title_sort implementation of a new protocol for direct identification from urine in the routine microbiological diagnosis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137483/
https://www.ncbi.nlm.nih.gov/pubmed/35625226
http://dx.doi.org/10.3390/antibiotics11050582
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