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Phytochemical Composition, Antioxidant and Antifungal Activity of Thymus capitatus, a Medicinal Plant Collected from Northern Morocco

Thymus capitatus is a Mediterranean endemic plant commonly known as “Zaïtra” in northern Morocco. As T. capitatus is widely used in traditional medicine and food, this present work aims to investigate the chemical compositions and biological activities of the T. capitatus leaves essential oil (TcLEO...

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Autores principales: Benoutman, Amina, Erbiai, El Hadi, Edderdaki, Fatima Zahra, Cherif, El Khalil, Saidi, Rabah, Lamrani, Zouhaire, Pintado, Manuela, Pinto, Eugénia, Esteves da Silva, Joaquim C. G., Maouni, Abdelfettah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137586/
https://www.ncbi.nlm.nih.gov/pubmed/35625325
http://dx.doi.org/10.3390/antibiotics11050681
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author Benoutman, Amina
Erbiai, El Hadi
Edderdaki, Fatima Zahra
Cherif, El Khalil
Saidi, Rabah
Lamrani, Zouhaire
Pintado, Manuela
Pinto, Eugénia
Esteves da Silva, Joaquim C. G.
Maouni, Abdelfettah
author_facet Benoutman, Amina
Erbiai, El Hadi
Edderdaki, Fatima Zahra
Cherif, El Khalil
Saidi, Rabah
Lamrani, Zouhaire
Pintado, Manuela
Pinto, Eugénia
Esteves da Silva, Joaquim C. G.
Maouni, Abdelfettah
author_sort Benoutman, Amina
collection PubMed
description Thymus capitatus is a Mediterranean endemic plant commonly known as “Zaïtra” in northern Morocco. As T. capitatus is widely used in traditional medicine and food, this present work aims to investigate the chemical compositions and biological activities of the T. capitatus leaves essential oil (TcLEO), acetonic (TcLAE), and methanolic extract (TcLME). The spectrophotometric determination demonstrated that T. capitatus is a natural source rich in phenolic contents (TPC) and flavonoid contents (TFC) and that TcLME revealed the highest TPC and TFC than TcLAE and TcLEO. The LC-MS analysis of phenolic compounds showed that paraben acid was predominant in both TcLME and TcLAE, followed by cinnamic acid and p-hydroxybenzoic acid. GC-MS analysis of the TcLEO revealed the presence of a total of 10 compounds, which were predominated by carvacrol. The antioxidant activity by ORAC was observed to be significantly higher in TcLEO and TcLAE than in TcLME. All samples used to assess DNA degradation effectively prevented DNA oxidation and, at the same time, had a prooxidant effect. The genotoxicity test showed that the T. capitatus were devoid of any mutagenic activity. Concerning antifungal activity, all samples were able to inhibit the growth of all microorganisms tested at low concentrations. TcLAE showed higher activity than TcLME, and in general, dermatophytes were more susceptible, being Microsporum canis the most sensitive one. Overall, the results obtained from this study confirm the wide uses of T. capitatus. Furthermore, the finding results suggest that the T. capitatus essential oil and extracts can be highly useful for pharmaceutical industries.
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spelling pubmed-91375862022-05-28 Phytochemical Composition, Antioxidant and Antifungal Activity of Thymus capitatus, a Medicinal Plant Collected from Northern Morocco Benoutman, Amina Erbiai, El Hadi Edderdaki, Fatima Zahra Cherif, El Khalil Saidi, Rabah Lamrani, Zouhaire Pintado, Manuela Pinto, Eugénia Esteves da Silva, Joaquim C. G. Maouni, Abdelfettah Antibiotics (Basel) Article Thymus capitatus is a Mediterranean endemic plant commonly known as “Zaïtra” in northern Morocco. As T. capitatus is widely used in traditional medicine and food, this present work aims to investigate the chemical compositions and biological activities of the T. capitatus leaves essential oil (TcLEO), acetonic (TcLAE), and methanolic extract (TcLME). The spectrophotometric determination demonstrated that T. capitatus is a natural source rich in phenolic contents (TPC) and flavonoid contents (TFC) and that TcLME revealed the highest TPC and TFC than TcLAE and TcLEO. The LC-MS analysis of phenolic compounds showed that paraben acid was predominant in both TcLME and TcLAE, followed by cinnamic acid and p-hydroxybenzoic acid. GC-MS analysis of the TcLEO revealed the presence of a total of 10 compounds, which were predominated by carvacrol. The antioxidant activity by ORAC was observed to be significantly higher in TcLEO and TcLAE than in TcLME. All samples used to assess DNA degradation effectively prevented DNA oxidation and, at the same time, had a prooxidant effect. The genotoxicity test showed that the T. capitatus were devoid of any mutagenic activity. Concerning antifungal activity, all samples were able to inhibit the growth of all microorganisms tested at low concentrations. TcLAE showed higher activity than TcLME, and in general, dermatophytes were more susceptible, being Microsporum canis the most sensitive one. Overall, the results obtained from this study confirm the wide uses of T. capitatus. Furthermore, the finding results suggest that the T. capitatus essential oil and extracts can be highly useful for pharmaceutical industries. MDPI 2022-05-18 /pmc/articles/PMC9137586/ /pubmed/35625325 http://dx.doi.org/10.3390/antibiotics11050681 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Benoutman, Amina
Erbiai, El Hadi
Edderdaki, Fatima Zahra
Cherif, El Khalil
Saidi, Rabah
Lamrani, Zouhaire
Pintado, Manuela
Pinto, Eugénia
Esteves da Silva, Joaquim C. G.
Maouni, Abdelfettah
Phytochemical Composition, Antioxidant and Antifungal Activity of Thymus capitatus, a Medicinal Plant Collected from Northern Morocco
title Phytochemical Composition, Antioxidant and Antifungal Activity of Thymus capitatus, a Medicinal Plant Collected from Northern Morocco
title_full Phytochemical Composition, Antioxidant and Antifungal Activity of Thymus capitatus, a Medicinal Plant Collected from Northern Morocco
title_fullStr Phytochemical Composition, Antioxidant and Antifungal Activity of Thymus capitatus, a Medicinal Plant Collected from Northern Morocco
title_full_unstemmed Phytochemical Composition, Antioxidant and Antifungal Activity of Thymus capitatus, a Medicinal Plant Collected from Northern Morocco
title_short Phytochemical Composition, Antioxidant and Antifungal Activity of Thymus capitatus, a Medicinal Plant Collected from Northern Morocco
title_sort phytochemical composition, antioxidant and antifungal activity of thymus capitatus, a medicinal plant collected from northern morocco
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137586/
https://www.ncbi.nlm.nih.gov/pubmed/35625325
http://dx.doi.org/10.3390/antibiotics11050681
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