Cargando…
Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria
The topographic features of surfaces are known to affect bacterial retention on a surface, but the precise mechanisms of this phenomenon are little understood. Four coccal-shaped bacteria, Staphylococcus sciuri, Streptococcus pyogenes, Micrococcus luteus, and Staphylococcus aureus, that organise in...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137600/ https://www.ncbi.nlm.nih.gov/pubmed/35625195 http://dx.doi.org/10.3390/antibiotics11050551 |
_version_ | 1784714417312628736 |
---|---|
author | Evans, Adele Slate, Anthony J. Tobin, Millie Lynch, Stephen Wilson Nieuwenhuis, Joels Verran, Joanna Kelly, Peter Whitehead, Kathryn A. |
author_facet | Evans, Adele Slate, Anthony J. Tobin, Millie Lynch, Stephen Wilson Nieuwenhuis, Joels Verran, Joanna Kelly, Peter Whitehead, Kathryn A. |
author_sort | Evans, Adele |
collection | PubMed |
description | The topographic features of surfaces are known to affect bacterial retention on a surface, but the precise mechanisms of this phenomenon are little understood. Four coccal-shaped bacteria, Staphylococcus sciuri, Streptococcus pyogenes, Micrococcus luteus, and Staphylococcus aureus, that organise in different cellular groupings (grape-like clusters, tetrad-arranging clusters, short chains, and diploid arrangement, respectively) were used. These differently grouped cells were used to determine how surface topography affected their distribution, density, dispersion, and clustering when retained on titanium surfaces with defined topographies. Titanium-coated surfaces that were smooth and had grooved features of 1.02 µm-wide, 0.21 µm-deep grooves, and 0.59 µm-wide, 0.17 µm-deep grooves were used. The average contact angle of the surfaces was 91°. All bacterial species were overall of a hydrophobic nature, although M. luteus was the least hydrophobic. It was demonstrated that the 1.02 µm-wide featured surface most affected Strep. pyogenes and S. sciuri, and hence the surfaces with the larger surface features most affected the cells with smaller dimensions. The 0.59 µm featured surface only affected the density of the bacteria, and it may be suggested that the surfaces with the smaller features reduced bacterial retention. These results demonstrate that the size of the topographical surface features affect the distribution, density, dispersion, and clustering of bacteria across surfaces, and this is related to the cellular organisation of the bacterial species. The results from this work inform how surface topographical and bacterial properties affect the distribution, density, dispersion, and clustering of bacterial retention. |
format | Online Article Text |
id | pubmed-9137600 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91376002022-05-28 Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria Evans, Adele Slate, Anthony J. Tobin, Millie Lynch, Stephen Wilson Nieuwenhuis, Joels Verran, Joanna Kelly, Peter Whitehead, Kathryn A. Antibiotics (Basel) Article The topographic features of surfaces are known to affect bacterial retention on a surface, but the precise mechanisms of this phenomenon are little understood. Four coccal-shaped bacteria, Staphylococcus sciuri, Streptococcus pyogenes, Micrococcus luteus, and Staphylococcus aureus, that organise in different cellular groupings (grape-like clusters, tetrad-arranging clusters, short chains, and diploid arrangement, respectively) were used. These differently grouped cells were used to determine how surface topography affected their distribution, density, dispersion, and clustering when retained on titanium surfaces with defined topographies. Titanium-coated surfaces that were smooth and had grooved features of 1.02 µm-wide, 0.21 µm-deep grooves, and 0.59 µm-wide, 0.17 µm-deep grooves were used. The average contact angle of the surfaces was 91°. All bacterial species were overall of a hydrophobic nature, although M. luteus was the least hydrophobic. It was demonstrated that the 1.02 µm-wide featured surface most affected Strep. pyogenes and S. sciuri, and hence the surfaces with the larger surface features most affected the cells with smaller dimensions. The 0.59 µm featured surface only affected the density of the bacteria, and it may be suggested that the surfaces with the smaller features reduced bacterial retention. These results demonstrate that the size of the topographical surface features affect the distribution, density, dispersion, and clustering of bacteria across surfaces, and this is related to the cellular organisation of the bacterial species. The results from this work inform how surface topographical and bacterial properties affect the distribution, density, dispersion, and clustering of bacterial retention. MDPI 2022-04-21 /pmc/articles/PMC9137600/ /pubmed/35625195 http://dx.doi.org/10.3390/antibiotics11050551 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Evans, Adele Slate, Anthony J. Tobin, Millie Lynch, Stephen Wilson Nieuwenhuis, Joels Verran, Joanna Kelly, Peter Whitehead, Kathryn A. Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria |
title | Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria |
title_full | Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria |
title_fullStr | Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria |
title_full_unstemmed | Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria |
title_short | Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria |
title_sort | multifractal analysis to determine the effect of surface topography on the distribution, density, dispersion and clustering of differently organised coccal-shaped bacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137600/ https://www.ncbi.nlm.nih.gov/pubmed/35625195 http://dx.doi.org/10.3390/antibiotics11050551 |
work_keys_str_mv | AT evansadele multifractalanalysistodeterminetheeffectofsurfacetopographyonthedistributiondensitydispersionandclusteringofdifferentlyorganisedcoccalshapedbacteria AT slateanthonyj multifractalanalysistodeterminetheeffectofsurfacetopographyonthedistributiondensitydispersionandclusteringofdifferentlyorganisedcoccalshapedbacteria AT tobinmillie multifractalanalysistodeterminetheeffectofsurfacetopographyonthedistributiondensitydispersionandclusteringofdifferentlyorganisedcoccalshapedbacteria AT lynchstephen multifractalanalysistodeterminetheeffectofsurfacetopographyonthedistributiondensitydispersionandclusteringofdifferentlyorganisedcoccalshapedbacteria AT wilsonnieuwenhuisjoels multifractalanalysistodeterminetheeffectofsurfacetopographyonthedistributiondensitydispersionandclusteringofdifferentlyorganisedcoccalshapedbacteria AT verranjoanna multifractalanalysistodeterminetheeffectofsurfacetopographyonthedistributiondensitydispersionandclusteringofdifferentlyorganisedcoccalshapedbacteria AT kellypeter multifractalanalysistodeterminetheeffectofsurfacetopographyonthedistributiondensitydispersionandclusteringofdifferentlyorganisedcoccalshapedbacteria AT whiteheadkathryna multifractalanalysistodeterminetheeffectofsurfacetopographyonthedistributiondensitydispersionandclusteringofdifferentlyorganisedcoccalshapedbacteria |