Cargando…
Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family
Antimicrobial susceptibility testing is necessary to carry out antimicrobial stewardship but a limited number of drugs belonging to each antimicrobial family has to be tested for technical limitations and economic resources. In this study, we have determined the minimal inhibitory concentration, usi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137606/ https://www.ncbi.nlm.nih.gov/pubmed/35625283 http://dx.doi.org/10.3390/antibiotics11050638 |
_version_ | 1784714418967281664 |
---|---|
author | Vilaró, Anna Novell, Elena Enrique-Tarancon, Vicens Balielles, Jordi Migura-García, Lourdes Fraile, Lorenzo |
author_facet | Vilaró, Anna Novell, Elena Enrique-Tarancon, Vicens Balielles, Jordi Migura-García, Lourdes Fraile, Lorenzo |
author_sort | Vilaró, Anna |
collection | PubMed |
description | Antimicrobial susceptibility testing is necessary to carry out antimicrobial stewardship but a limited number of drugs belonging to each antimicrobial family has to be tested for technical limitations and economic resources. In this study, we have determined the minimal inhibitory concentration, using microdilution following international standards (CLSI), for 490 Actinobacillus pleuropneumoniae, 285 Pasteurella multocida, 73 Bordetella bronchiseptica, 398 Streptococcus suis and 1571 Escherichia coli strains from clinical cases collected in Spain between 2018 and 2020. The antimicrobial susceptibility pattern was deciphered using a principal component analysis for each bacterium and a matrix correlation (high > 0.8, medium 0.5–0.8 and low < 0.5) was obtained for each pair of antimicrobials. No significant associations were observed between MIC patterns for different antimicrobial families, suggesting that co-selection mechanisms are not generally present in these porcine pathogens. However, a high correlation was observed between the fluroquinolones (marbofloxacin and enrofloxacin) for all mentioned pathogens and for ceftiofur and cefquinome for E. coli and S. suis. Moreover, a significant association was also observed for tetracyclines (doxycycline and oxytetracycline) and B. bronchiseptica and tildipirosin/tulathromycin for P. multocida. These results suggest that generally, a representative drug per antimicrobial class cannot be selected, however, for some drug–bug combinations, MIC values from one representative drug could be extrapolated to the whole antimicrobial family. |
format | Online Article Text |
id | pubmed-9137606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91376062022-05-28 Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family Vilaró, Anna Novell, Elena Enrique-Tarancon, Vicens Balielles, Jordi Migura-García, Lourdes Fraile, Lorenzo Antibiotics (Basel) Article Antimicrobial susceptibility testing is necessary to carry out antimicrobial stewardship but a limited number of drugs belonging to each antimicrobial family has to be tested for technical limitations and economic resources. In this study, we have determined the minimal inhibitory concentration, using microdilution following international standards (CLSI), for 490 Actinobacillus pleuropneumoniae, 285 Pasteurella multocida, 73 Bordetella bronchiseptica, 398 Streptococcus suis and 1571 Escherichia coli strains from clinical cases collected in Spain between 2018 and 2020. The antimicrobial susceptibility pattern was deciphered using a principal component analysis for each bacterium and a matrix correlation (high > 0.8, medium 0.5–0.8 and low < 0.5) was obtained for each pair of antimicrobials. No significant associations were observed between MIC patterns for different antimicrobial families, suggesting that co-selection mechanisms are not generally present in these porcine pathogens. However, a high correlation was observed between the fluroquinolones (marbofloxacin and enrofloxacin) for all mentioned pathogens and for ceftiofur and cefquinome for E. coli and S. suis. Moreover, a significant association was also observed for tetracyclines (doxycycline and oxytetracycline) and B. bronchiseptica and tildipirosin/tulathromycin for P. multocida. These results suggest that generally, a representative drug per antimicrobial class cannot be selected, however, for some drug–bug combinations, MIC values from one representative drug could be extrapolated to the whole antimicrobial family. MDPI 2022-05-10 /pmc/articles/PMC9137606/ /pubmed/35625283 http://dx.doi.org/10.3390/antibiotics11050638 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vilaró, Anna Novell, Elena Enrique-Tarancon, Vicens Balielles, Jordi Migura-García, Lourdes Fraile, Lorenzo Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
title | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
title_full | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
title_fullStr | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
title_full_unstemmed | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
title_short | Antimicrobial Susceptibility Testing of Porcine Bacterial Pathogens: Investigating the Prospect of Testing a Representative Drug for Each Antimicrobial Family |
title_sort | antimicrobial susceptibility testing of porcine bacterial pathogens: investigating the prospect of testing a representative drug for each antimicrobial family |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137606/ https://www.ncbi.nlm.nih.gov/pubmed/35625283 http://dx.doi.org/10.3390/antibiotics11050638 |
work_keys_str_mv | AT vilaroanna antimicrobialsusceptibilitytestingofporcinebacterialpathogensinvestigatingtheprospectoftestingarepresentativedrugforeachantimicrobialfamily AT novellelena antimicrobialsusceptibilitytestingofporcinebacterialpathogensinvestigatingtheprospectoftestingarepresentativedrugforeachantimicrobialfamily AT enriquetaranconvicens antimicrobialsusceptibilitytestingofporcinebacterialpathogensinvestigatingtheprospectoftestingarepresentativedrugforeachantimicrobialfamily AT baliellesjordi antimicrobialsusceptibilitytestingofporcinebacterialpathogensinvestigatingtheprospectoftestingarepresentativedrugforeachantimicrobialfamily AT miguragarcialourdes antimicrobialsusceptibilitytestingofporcinebacterialpathogensinvestigatingtheprospectoftestingarepresentativedrugforeachantimicrobialfamily AT frailelorenzo antimicrobialsusceptibilitytestingofporcinebacterialpathogensinvestigatingtheprospectoftestingarepresentativedrugforeachantimicrobialfamily |