Histopathology of Corn Plants Infected by Endophytic Fungi

SIMPLE SUMMARY: Endophytic fungi are fungi that live all or part of their life cycles within the tissues of their hosts. Although several endophyte-plant interactions are non-pathogenic in nature, it has been reported that upon infection, some endophytic fungi are able to adopt a pathogenic status resulting in disease symptoms in their host plants, ranging in severity from mild to severe. In the present study, light microscopy and transmission electron microscopy were used to investigate the tissue colonization and cell damage of corn plants by three endophytic fungi, Fusarium verticillioides, Fusarium sacchari, and Penicillium citrinum, causing disease symptoms in the roots, stems, and leaves of infected corn plants. Tissue deterioration and cell collapse were produced by endophytic F. verticillioides, while significant cell proliferation and wall thickening were observed in corn plants infected by endophytic F. sacchari. Corn plants infected by P. citrinum showed reductions in cell diameter of the vascular bundles of infected corn tissues. The ability of endophytic fungi recovered from healthy corn plants to cause disease when inoculated in healthy tissues of other corn plants signifies their importance as determinants of corn health and productivity. ABSTRACT: Endophytic fungi inhabiting plant tissues show extensive functional diversity, ranging from mutualism to pathogenicity. The present study evaluated the histological responses of corn plants to colonization by three species of endophytic fungi isolated from corn. Corn seedlings were inoculated with 1 × 10(6) conidia per mL spore suspensions of endophytic Fusarium verticillioides, Fusarium sacchari, and Penicillium citrinum and observed for 14 days for the emergence of disease symptoms. Histological examination of diseased root, stem, and leaf tissues was conducted using light and transmission electron microscopy. The results indicated that the mean diameters of root phloem, stem vascular bundles, and leaf vascular bundles, of corn plants infected with endophytic P. citrinum (18.91 µm, 146.96 µm, and 107.86 µm, respectively), F. verticillioides (18.75 µm, 85.45 µm, and 118.24 µm, respectively), and F. sacchari (24.15 µm root phloem, and 98.90 µm stem vascular bundle diameters), were significantly lower than the root phloem (33.68 µm), stem vascular bundle (186.77 µm), and leaf vascular bundle (155.88 µm) of the uninfected corn plants (p ≤ 0.05). Endophytic F. verticillioides was the most virulent, resulting in severe degradation and the eventual collapse of infected plant tissues. The study showed that endophytic fungi recovered from corn plants are capable of initiating significant disease responses in infected corn tissues.