Canine Neonatal Assessment by Vitality Score, Amniotic Fluid, Urine, and Umbilical Cord Blood Analysis of Glucose, Lactate, and Cortisol: Possible Influence of Parturition Type?

SIMPLE SUMMARY: Parturition as a stressful event may influence puppies’ neonatal morbidity and mortality. The purpose of this study was to investigate the impact of parturition type on stress in newborn puppies, their weight gains, and survival in the first week postpartum. One hundred and twenty-three puppies were divided into three groups: vaginal parturition, emergency, and elective cesarean section. The Apgar score was assessed 5, 15, and 60 min postpartum, and samples of amniotic fluid, umbilical blood, and urine were collected for lactate, glucose, and cortisol concentration measurements. Although emergency cesarean section puppies had the highest cortisol concentration of all groups, their Apgar score at 5 min postpartum was comparable to the vaginal parturition group, which had the highest lactate levels. There were no significant differences between groups regarding relative growth rate. The type of parturition had no impact on puppies’ survival in our study, but supportive treatment was provided for non-vital puppies in stress. Non-invasive analysis of amniotic fluid and/or urine could help in the assessment of the neonatal vitality. ABSTRACT: The objective of this study was to investigate the impact of parturition type on vitality in newborn puppies, their weight gains, and survival in the first week postpartum. One hundred and twenty-three puppies were divided in three groups: vaginal parturition (VP), emergency (EM-CS), and elective cesarean section (EL-CS). Apgar scores were assessed 5, 15, and 60 min postpartum. Lactate and glucose concentrations were measured in amniotic fluid and umbilical blood; cortisol concentrations were measured in amniotic fluid and puppy urine. Puppies’ weight gain was tracked daily for 7 days postpartum. Apgar score at 5 and 15 min was significantly better in the VP group. EL-CS puppies had significantly lower umbilical blood and amniotic fluid lactate concentrations compared to the VP group, which also had higher umbilical blood lactate concentration than EM-CS puppies. The cortisol concentration in the amniotic fluid and in urine differed significantly between the groups, with the highest concentration in the EM-CS, followed by the VP group. Glucose concentration in amniotic fluid was higher in the VP group than EM-CS group. The type of parturition had no impact on puppies’ weight gain or their survival at birth; however, supportive treatment was provided for non-vital puppies. Non-invasive analysis of puppies’ fluids could help in the assessment of the neonatal vitality.