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Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells

SIMPLE SUMMARY: Cat’s health is impacted by several diseases and lesions for which cell therapy could be an interesting treatment. Mesenchymal stem cells or adult stem cells are found in developed tissue. Olfactory mucosa contains stem cells called olfactory ecto-mesenchymal stem cells which have al...

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Autores principales: Mollichella, Marie-Laure, Mechin, Violaine, Royer, Dany, Pageat, Patrick, Asproni, Pietro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137790/
https://www.ncbi.nlm.nih.gov/pubmed/35625130
http://dx.doi.org/10.3390/ani12101284
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author Mollichella, Marie-Laure
Mechin, Violaine
Royer, Dany
Pageat, Patrick
Asproni, Pietro
author_facet Mollichella, Marie-Laure
Mechin, Violaine
Royer, Dany
Pageat, Patrick
Asproni, Pietro
author_sort Mollichella, Marie-Laure
collection PubMed
description SIMPLE SUMMARY: Cat’s health is impacted by several diseases and lesions for which cell therapy could be an interesting treatment. Mesenchymal stem cells or adult stem cells are found in developed tissue. Olfactory mucosa contains stem cells called olfactory ecto-mesenchymal stem cells which have already been isolated from various animals as dogs and horses. The aim of this study was to evaluate the feasibility of collecting olfactory ecto-mesenchymal stem cells in cats. For that purpose, four cats were biopsied; the cells were collected and characterized. They show stemness features and differentiation capabilities as all the other mammals previously studied. Therefore, olfactory ecto-mesenchymal stem cells could be a promising tool for feline regenerative medicine. ABSTRACT: The olfactory mucosa contains olfactory ecto-mesenchymal stem cells (OE-MSCs) which show stemness features, multipotency capabilities, and have a therapeutic potential. The OE-MSCs have already been collected and isolated from various mammals. The aim of this study was to evaluate the feasibility of collecting, purifying and amplifying OE-MSCs from the cat nasal cavity. Four cats were included in the study. Biopsies of olfactory mucosa were performed on anesthetized animals. Then, the olfactory OE-MSCs were isolated, and their stemness features as well as their mesodermal differentiation capabilities were characterized. Olfactory mucosa biopsies were successfully performed in all subjects. From these biopsies, cellular populations were rapidly generated, presenting various stemness features, such as a fibroblast-like morphology, nestin and MAP2 expression, and sphere and colony formation. These cells could differentiate into neural and mesodermal lineages. This report shows for the first time that the isolation of OE-MSCs from cat olfactory mucosa is possible. These cells showed stemness features and multilineage differentiation capabilities, indicating they may be a promising tool for autologous grafts and feline regenerative medicine.
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spelling pubmed-91377902022-05-28 Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells Mollichella, Marie-Laure Mechin, Violaine Royer, Dany Pageat, Patrick Asproni, Pietro Animals (Basel) Communication SIMPLE SUMMARY: Cat’s health is impacted by several diseases and lesions for which cell therapy could be an interesting treatment. Mesenchymal stem cells or adult stem cells are found in developed tissue. Olfactory mucosa contains stem cells called olfactory ecto-mesenchymal stem cells which have already been isolated from various animals as dogs and horses. The aim of this study was to evaluate the feasibility of collecting olfactory ecto-mesenchymal stem cells in cats. For that purpose, four cats were biopsied; the cells were collected and characterized. They show stemness features and differentiation capabilities as all the other mammals previously studied. Therefore, olfactory ecto-mesenchymal stem cells could be a promising tool for feline regenerative medicine. ABSTRACT: The olfactory mucosa contains olfactory ecto-mesenchymal stem cells (OE-MSCs) which show stemness features, multipotency capabilities, and have a therapeutic potential. The OE-MSCs have already been collected and isolated from various mammals. The aim of this study was to evaluate the feasibility of collecting, purifying and amplifying OE-MSCs from the cat nasal cavity. Four cats were included in the study. Biopsies of olfactory mucosa were performed on anesthetized animals. Then, the olfactory OE-MSCs were isolated, and their stemness features as well as their mesodermal differentiation capabilities were characterized. Olfactory mucosa biopsies were successfully performed in all subjects. From these biopsies, cellular populations were rapidly generated, presenting various stemness features, such as a fibroblast-like morphology, nestin and MAP2 expression, and sphere and colony formation. These cells could differentiate into neural and mesodermal lineages. This report shows for the first time that the isolation of OE-MSCs from cat olfactory mucosa is possible. These cells showed stemness features and multilineage differentiation capabilities, indicating they may be a promising tool for autologous grafts and feline regenerative medicine. MDPI 2022-05-17 /pmc/articles/PMC9137790/ /pubmed/35625130 http://dx.doi.org/10.3390/ani12101284 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mollichella, Marie-Laure
Mechin, Violaine
Royer, Dany
Pageat, Patrick
Asproni, Pietro
Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells
title Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells
title_full Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells
title_fullStr Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells
title_full_unstemmed Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells
title_short Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells
title_sort isolation and characterization of cat olfactory ecto-mesenchymal stem cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137790/
https://www.ncbi.nlm.nih.gov/pubmed/35625130
http://dx.doi.org/10.3390/ani12101284
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