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An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp (Ctenopharyngodon idella)

Mannan oligosaccharides (MOS) are a type of functional oligosaccharide which have received increased attention because of their beneficial effects on fish intestinal health. However, intestinal structural integrity is a necessary prerequisite for intestinal health. This study focused on exploring th...

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Autores principales: Lu, Zhi-Yuan, Feng, Lin, Jiang, Wei-Dan, Wu, Pei, Liu, Yang, Jin, Xiao-Wan, Ren, Hong-Mei, Kuang, Sheng-Yao, Li, Shu-Wei, Tang, Ling, Zhang, Lu, Mi, Hai-Feng, Zhou, Xiao-Qiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137958/
https://www.ncbi.nlm.nih.gov/pubmed/35624670
http://dx.doi.org/10.3390/antiox11050806
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author Lu, Zhi-Yuan
Feng, Lin
Jiang, Wei-Dan
Wu, Pei
Liu, Yang
Jin, Xiao-Wan
Ren, Hong-Mei
Kuang, Sheng-Yao
Li, Shu-Wei
Tang, Ling
Zhang, Lu
Mi, Hai-Feng
Zhou, Xiao-Qiu
author_facet Lu, Zhi-Yuan
Feng, Lin
Jiang, Wei-Dan
Wu, Pei
Liu, Yang
Jin, Xiao-Wan
Ren, Hong-Mei
Kuang, Sheng-Yao
Li, Shu-Wei
Tang, Ling
Zhang, Lu
Mi, Hai-Feng
Zhou, Xiao-Qiu
author_sort Lu, Zhi-Yuan
collection PubMed
description Mannan oligosaccharides (MOS) are a type of functional oligosaccharide which have received increased attention because of their beneficial effects on fish intestinal health. However, intestinal structural integrity is a necessary prerequisite for intestinal health. This study focused on exploring the protective effects of dietary MOS supplementation on the grass carp’s (Ctenopharyngodon idella) intestinal structural integrity (including tight junction (TJ) and adherent junction (AJ)) and its related signalling molecule mechanism. A total of 540 grass carp (215.85 ± 0.30 g) were fed six diets containing graded levels of dietary MOS supplementation (0, 200, 400, 600, 800 and 1000 mg/kg) for 60 days. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila for 14 days. We used ELISA, spectrophotometry, transmission electron microscope, immunohistochemistry, qRT-PCR and Western blotting to determine the effect of dietary MOS supplementation on intestinal structural integrity and antioxidant capacity. The results revealed that dietary MOS supplementation protected the microvillus of the intestine; reduced serum diamine oxidase and d-lactate levels (p < 0.05); enhanced intestinal total antioxidant capacity (p < 0.01); up-regulated most intestinal TJ and AJ mRNA levels; and decreased GTP-RhoA protein levels (p < 0.01). In addition, we also found several interesting results suggesting that MOS supplementation has no effects on ZO-2 and Claudin-15b. Overall, these findings suggested that dietary MOS supplementation could protect intestinal ultrastructure, reduce intestinal mucosal permeability and maintain intestinal structural integrity via inhibiting MLCK and RhoA/ROCK signalling pathways.
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spelling pubmed-91379582022-05-28 An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp (Ctenopharyngodon idella) Lu, Zhi-Yuan Feng, Lin Jiang, Wei-Dan Wu, Pei Liu, Yang Jin, Xiao-Wan Ren, Hong-Mei Kuang, Sheng-Yao Li, Shu-Wei Tang, Ling Zhang, Lu Mi, Hai-Feng Zhou, Xiao-Qiu Antioxidants (Basel) Article Mannan oligosaccharides (MOS) are a type of functional oligosaccharide which have received increased attention because of their beneficial effects on fish intestinal health. However, intestinal structural integrity is a necessary prerequisite for intestinal health. This study focused on exploring the protective effects of dietary MOS supplementation on the grass carp’s (Ctenopharyngodon idella) intestinal structural integrity (including tight junction (TJ) and adherent junction (AJ)) and its related signalling molecule mechanism. A total of 540 grass carp (215.85 ± 0.30 g) were fed six diets containing graded levels of dietary MOS supplementation (0, 200, 400, 600, 800 and 1000 mg/kg) for 60 days. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila for 14 days. We used ELISA, spectrophotometry, transmission electron microscope, immunohistochemistry, qRT-PCR and Western blotting to determine the effect of dietary MOS supplementation on intestinal structural integrity and antioxidant capacity. The results revealed that dietary MOS supplementation protected the microvillus of the intestine; reduced serum diamine oxidase and d-lactate levels (p < 0.05); enhanced intestinal total antioxidant capacity (p < 0.01); up-regulated most intestinal TJ and AJ mRNA levels; and decreased GTP-RhoA protein levels (p < 0.01). In addition, we also found several interesting results suggesting that MOS supplementation has no effects on ZO-2 and Claudin-15b. Overall, these findings suggested that dietary MOS supplementation could protect intestinal ultrastructure, reduce intestinal mucosal permeability and maintain intestinal structural integrity via inhibiting MLCK and RhoA/ROCK signalling pathways. MDPI 2022-04-20 /pmc/articles/PMC9137958/ /pubmed/35624670 http://dx.doi.org/10.3390/antiox11050806 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Zhi-Yuan
Feng, Lin
Jiang, Wei-Dan
Wu, Pei
Liu, Yang
Jin, Xiao-Wan
Ren, Hong-Mei
Kuang, Sheng-Yao
Li, Shu-Wei
Tang, Ling
Zhang, Lu
Mi, Hai-Feng
Zhou, Xiao-Qiu
An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp (Ctenopharyngodon idella)
title An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp (Ctenopharyngodon idella)
title_full An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp (Ctenopharyngodon idella)
title_fullStr An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp (Ctenopharyngodon idella)
title_full_unstemmed An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp (Ctenopharyngodon idella)
title_short An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides after Aeromonas hydrophila Infection in Grass Carp (Ctenopharyngodon idella)
title_sort antioxidant supplement function exploration: rescue of intestinal structure injury by mannan oligosaccharides after aeromonas hydrophila infection in grass carp (ctenopharyngodon idella)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137958/
https://www.ncbi.nlm.nih.gov/pubmed/35624670
http://dx.doi.org/10.3390/antiox11050806
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