Cargando…

Evaluation of a Frozen Micro-Agar Plates of MAPt Antibiotic Susceptibility Test for Enhanced Bioterror Preparedness

There is an urgent need for rapid antibiotic susceptibility tests to improve clinical treatment and to support antibiotic stewardship, especially concerning the emergence of multi-drug-resistant bacteria. Nowadays this need is even more profound due to progress in synthetic biology procedures that m...

Descripción completa

Detalles Bibliográficos
Autores principales: Rotem, Shahar, Shifman, Ohad, Aloni-Grinstein, Ronit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137970/
https://www.ncbi.nlm.nih.gov/pubmed/35625224
http://dx.doi.org/10.3390/antibiotics11050580
_version_ 1784714511080488960
author Rotem, Shahar
Shifman, Ohad
Aloni-Grinstein, Ronit
author_facet Rotem, Shahar
Shifman, Ohad
Aloni-Grinstein, Ronit
author_sort Rotem, Shahar
collection PubMed
description There is an urgent need for rapid antibiotic susceptibility tests to improve clinical treatment and to support antibiotic stewardship, especially concerning the emergence of multi-drug-resistant bacteria. Nowadays this need is even more profound due to progress in synthetic biology procedures that may facilitate the malicious preparation of engineered antibiotic-resistant pathogens. We recently described a novel, rapid, simple, specific, and sensitive method named a Micro-Agar-PCR-test (MAPt) and showed its performance on clinical as well as environmental samples. The method does not require any isolation or purification steps and is applicable to a wide range of bacterial concentrations, thus allowing a short time to respond within a bioterror event (5–7 h for B. anthracis, 10–12 h for Y. pestis, and 16 h for F. tularensis). Ready-to-use reagents for this assay may add a level of preparedness. We examined the option of freezing pre-prepared MAPt agar plates and thawing them upon need. Our results show that adequate minimal inhibitory concentration (MIC) values are obtained with the use of thawed 6- and 12-month frozen agar plates. The ability to store MAPt micro-agar plates at −70 °C for a year, together with all other reagents required for MAPt, holds a great advantage for bioterror preparedness.
format Online
Article
Text
id pubmed-9137970
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91379702022-05-28 Evaluation of a Frozen Micro-Agar Plates of MAPt Antibiotic Susceptibility Test for Enhanced Bioterror Preparedness Rotem, Shahar Shifman, Ohad Aloni-Grinstein, Ronit Antibiotics (Basel) Brief Report There is an urgent need for rapid antibiotic susceptibility tests to improve clinical treatment and to support antibiotic stewardship, especially concerning the emergence of multi-drug-resistant bacteria. Nowadays this need is even more profound due to progress in synthetic biology procedures that may facilitate the malicious preparation of engineered antibiotic-resistant pathogens. We recently described a novel, rapid, simple, specific, and sensitive method named a Micro-Agar-PCR-test (MAPt) and showed its performance on clinical as well as environmental samples. The method does not require any isolation or purification steps and is applicable to a wide range of bacterial concentrations, thus allowing a short time to respond within a bioterror event (5–7 h for B. anthracis, 10–12 h for Y. pestis, and 16 h for F. tularensis). Ready-to-use reagents for this assay may add a level of preparedness. We examined the option of freezing pre-prepared MAPt agar plates and thawing them upon need. Our results show that adequate minimal inhibitory concentration (MIC) values are obtained with the use of thawed 6- and 12-month frozen agar plates. The ability to store MAPt micro-agar plates at −70 °C for a year, together with all other reagents required for MAPt, holds a great advantage for bioterror preparedness. MDPI 2022-04-26 /pmc/articles/PMC9137970/ /pubmed/35625224 http://dx.doi.org/10.3390/antibiotics11050580 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Rotem, Shahar
Shifman, Ohad
Aloni-Grinstein, Ronit
Evaluation of a Frozen Micro-Agar Plates of MAPt Antibiotic Susceptibility Test for Enhanced Bioterror Preparedness
title Evaluation of a Frozen Micro-Agar Plates of MAPt Antibiotic Susceptibility Test for Enhanced Bioterror Preparedness
title_full Evaluation of a Frozen Micro-Agar Plates of MAPt Antibiotic Susceptibility Test for Enhanced Bioterror Preparedness
title_fullStr Evaluation of a Frozen Micro-Agar Plates of MAPt Antibiotic Susceptibility Test for Enhanced Bioterror Preparedness
title_full_unstemmed Evaluation of a Frozen Micro-Agar Plates of MAPt Antibiotic Susceptibility Test for Enhanced Bioterror Preparedness
title_short Evaluation of a Frozen Micro-Agar Plates of MAPt Antibiotic Susceptibility Test for Enhanced Bioterror Preparedness
title_sort evaluation of a frozen micro-agar plates of mapt antibiotic susceptibility test for enhanced bioterror preparedness
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137970/
https://www.ncbi.nlm.nih.gov/pubmed/35625224
http://dx.doi.org/10.3390/antibiotics11050580
work_keys_str_mv AT rotemshahar evaluationofafrozenmicroagarplatesofmaptantibioticsusceptibilitytestforenhancedbioterrorpreparedness
AT shifmanohad evaluationofafrozenmicroagarplatesofmaptantibioticsusceptibilitytestforenhancedbioterrorpreparedness
AT alonigrinsteinronit evaluationofafrozenmicroagarplatesofmaptantibioticsusceptibilitytestforenhancedbioterrorpreparedness