Dietary Chitosan Supplementation Improved Egg Production and Antioxidative Function in Laying Breeders

SIMPLE SUMMARY: Chitosan is a natural, non-toxic and biodegradable compound, which has antibacterial, antioxidant and anti-tumor properties. Several studies have shown that chitosan also improve the antioxidant capacity of poultry. Recent research showed that chitosan decreased oxidative damage by activating the nuclear factor erythroid-2 related factor 2 pathway, then elevated the meat quality of broilers. Egg breeders are susceptible to oxidative stress during peak egg production, which increase their susceptibility to diseases and lead performance decline. In addition, previous reports on the effect of chitosan on poultry production performance were inconsistent. Based on above reports, this study explored whether chitosan could promote the production performance, and antioxidant defense of laying hens by affecting the nuclear factor erythroid-2 related factor 2 pathway. The results showed that addition chitosan to layer hen diet could increase egg production and feed conversion ratio, and the effect was better at the level of 250~500 mg/kg; as well as, chitosan promoted the antioxidant status in serum, liver and duodenum tissues and the effect was better at the level of 500 mg/kg. Chitosan was likely to increase antioxidant enzyme activities by enhancing the expression of nuclear factor erythroid-2 related factor 2, thereby improving the antioxidant capacity of laying breeders. ABSTRACT: This study was conducted to explore the dietary effect of chitosan on the production performance, and antioxidative enzyme activities and corresponding gene expression in the liver and duodenum of laying breeders. A total of 450 laying breeders (92.44% ± 0.030% of hen-day egg production) were randomly assigned to five dietary treatments fed 8 weeks: maize-soybean meal as the basal control diet and the basal diet containing 250, 500, 1000 and 2000 mg/kg of chitosan, respectively. Each treatment was randomly divided into 6 equal replicates, with 15 laying breeders in each replicate. The results showed that dietary chitosan could increase hen-day egg production and feed conversion ratio, especially at the level of 250~500 mg/kg; however, chitosan had no prominent effect on feed intake and average egg weight. Dietary chitosan could dose-dependently promote the antioxidant status in serum, liver and duodenum of layer breeders. It has a better promotion effect at the level of 500 mg/kg; however, the effect was weakened at the level of 2000 mg/kg. Chitosan was likely to enhance the gene expression and activities of Nrf2-mediated phase II detoxification enzyme by up-regulating the expression of Nrf2, thereby improving the antioxidant capacity of laying breeder hens.