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Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity

The biological synthesis of silver nanoparticles (AgNPs) for medical, environmental, and industrial applications is considered an alternative to chemical synthesis methods. Additionally, the reducing, capping, and stabilizing molecules produced by the organisms can play a key role in the further act...

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Autores principales: Hermosilla, Edward, Díaz, Marcela, Vera, Joelis, Seabra, Amedea B., Tortella, Gonzalo, Parada, Javiera, Rubilar, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138036/
https://www.ncbi.nlm.nih.gov/pubmed/35625266
http://dx.doi.org/10.3390/antibiotics11050622
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author Hermosilla, Edward
Díaz, Marcela
Vera, Joelis
Seabra, Amedea B.
Tortella, Gonzalo
Parada, Javiera
Rubilar, Olga
author_facet Hermosilla, Edward
Díaz, Marcela
Vera, Joelis
Seabra, Amedea B.
Tortella, Gonzalo
Parada, Javiera
Rubilar, Olga
author_sort Hermosilla, Edward
collection PubMed
description The biological synthesis of silver nanoparticles (AgNPs) for medical, environmental, and industrial applications is considered an alternative to chemical synthesis methods. Additionally, the reducing, capping, and stabilizing molecules produced by the organisms can play a key role in the further activity of AgNPs. In this work, we evaluated the synthesis of AgNPs by four molecular weight fractions (S1: <10 kDa, S2: 10 to 30 kDa, S3: 30 to 50 kDa, and S4: >50 kDa) of mycelia-free aqueous extract produced by the white-rot fungus Stereum hirsutum and their effect on the antimicrobial activity against Pseudomonas syringae and photocatalytic decolorization of nine synthetic dyes exposed to sunlight radiation. All synthesis assay fractions showed the characteristic surface plasmon resonance (SPR) with 403 to 421 nm peaks. TEM analysis of synthesized AgNPs showed different sizes: the whole mycelia-free extracts S0 (13.8 nm), S1 (9.06 nm), S2 (10.47 nm), S3 (22.48 nm), and S4 (16.92 nm) fractions. The results of disk diffusion assays showed an inverse relation between antimicrobial activity and the molecular weight of compounds present in the mycelia-free aqueous extract used to synthesize AgNPs. The AgNPs synthesized by S0 (14.3 mm) and S1(14.2 mm) generated the highest inhibition diameter of P. syringae growth. By contrast, in the photocatalytic assays, the AgNPs synthesized by the S2 fraction showed the highest discoloration in all the dyes tested, reaching 100% of the discoloration of basic dyes after 2 h of sunlight exposure. The maximum discoloration observed in reactive and acid dyes was 53.2% and 65.3%, respectively. This differentiation in the antimicrobial and photocatalytic activity of AgNPs could be attributed to the capping effect of the molecules present in the extract fractions. Therefore, the molecular separation of synthesis extract enables the specific activities of the AgNPs to be enhanced.
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spelling pubmed-91380362022-05-28 Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity Hermosilla, Edward Díaz, Marcela Vera, Joelis Seabra, Amedea B. Tortella, Gonzalo Parada, Javiera Rubilar, Olga Antibiotics (Basel) Article The biological synthesis of silver nanoparticles (AgNPs) for medical, environmental, and industrial applications is considered an alternative to chemical synthesis methods. Additionally, the reducing, capping, and stabilizing molecules produced by the organisms can play a key role in the further activity of AgNPs. In this work, we evaluated the synthesis of AgNPs by four molecular weight fractions (S1: <10 kDa, S2: 10 to 30 kDa, S3: 30 to 50 kDa, and S4: >50 kDa) of mycelia-free aqueous extract produced by the white-rot fungus Stereum hirsutum and their effect on the antimicrobial activity against Pseudomonas syringae and photocatalytic decolorization of nine synthetic dyes exposed to sunlight radiation. All synthesis assay fractions showed the characteristic surface plasmon resonance (SPR) with 403 to 421 nm peaks. TEM analysis of synthesized AgNPs showed different sizes: the whole mycelia-free extracts S0 (13.8 nm), S1 (9.06 nm), S2 (10.47 nm), S3 (22.48 nm), and S4 (16.92 nm) fractions. The results of disk diffusion assays showed an inverse relation between antimicrobial activity and the molecular weight of compounds present in the mycelia-free aqueous extract used to synthesize AgNPs. The AgNPs synthesized by S0 (14.3 mm) and S1(14.2 mm) generated the highest inhibition diameter of P. syringae growth. By contrast, in the photocatalytic assays, the AgNPs synthesized by the S2 fraction showed the highest discoloration in all the dyes tested, reaching 100% of the discoloration of basic dyes after 2 h of sunlight exposure. The maximum discoloration observed in reactive and acid dyes was 53.2% and 65.3%, respectively. This differentiation in the antimicrobial and photocatalytic activity of AgNPs could be attributed to the capping effect of the molecules present in the extract fractions. Therefore, the molecular separation of synthesis extract enables the specific activities of the AgNPs to be enhanced. MDPI 2022-05-05 /pmc/articles/PMC9138036/ /pubmed/35625266 http://dx.doi.org/10.3390/antibiotics11050622 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hermosilla, Edward
Díaz, Marcela
Vera, Joelis
Seabra, Amedea B.
Tortella, Gonzalo
Parada, Javiera
Rubilar, Olga
Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity
title Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity
title_full Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity
title_fullStr Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity
title_full_unstemmed Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity
title_short Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity
title_sort molecular weight identification of compounds involved in the fungal synthesis of agnps: effect on antimicrobial and photocatalytic activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138036/
https://www.ncbi.nlm.nih.gov/pubmed/35625266
http://dx.doi.org/10.3390/antibiotics11050622
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