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Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms
C. albicans and C. parapsilosis are biofilm-forming yeasts responsible for bloodstream infections that can cause death. Synthetic antimicrobial peptides (SAMPs) are considered to be new weapons to combat these infections, alone or combined with drugs. Here, two SAMPs, called Mo-CBP(3)-PepI and Mo-CB...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138075/ https://www.ncbi.nlm.nih.gov/pubmed/35625197 http://dx.doi.org/10.3390/antibiotics11050553 |
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author | Bezerra, Leandro P. Freitas, Cleverson D. T. Silva, Ayrles F. B. Amaral, Jackson L. Neto, Nilton A. S. Silva, Rafael G. G. Parra, Aura L. C. Goldman, Gustavo H. Oliveira, Jose T. A. Mesquita, Felipe P. Souza, Pedro F. N. |
author_facet | Bezerra, Leandro P. Freitas, Cleverson D. T. Silva, Ayrles F. B. Amaral, Jackson L. Neto, Nilton A. S. Silva, Rafael G. G. Parra, Aura L. C. Goldman, Gustavo H. Oliveira, Jose T. A. Mesquita, Felipe P. Souza, Pedro F. N. |
author_sort | Bezerra, Leandro P. |
collection | PubMed |
description | C. albicans and C. parapsilosis are biofilm-forming yeasts responsible for bloodstream infections that can cause death. Synthetic antimicrobial peptides (SAMPs) are considered to be new weapons to combat these infections, alone or combined with drugs. Here, two SAMPs, called Mo-CBP(3)-PepI and Mo-CBP(3)-PepIII, were tested alone or combined with nystatin (NYS) and itraconazole (ITR) against C. albicans and C. parapsilosis biofilms. Furthermore, the mechanism of antibiofilm activity was evaluated by fluorescence and scanning electron microscopies. When combined with SAMPs, the results revealed a 2- to 4-fold improvement of NYS and ITR antibiofilm activity. Microscopic analyses showed cell membrane and wall damage and ROS overproduction, which caused leakage of internal content and cell death. Taken together, these results suggest the potential of Mo-CBP(3)-PepI and Mo-CBP(3)-PepIII as new drugs and adjuvants to increase the activity of conventional drugs for the treatment of clinical infections caused by C. albicans and C. parapsilosis. |
format | Online Article Text |
id | pubmed-9138075 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91380752022-05-28 Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms Bezerra, Leandro P. Freitas, Cleverson D. T. Silva, Ayrles F. B. Amaral, Jackson L. Neto, Nilton A. S. Silva, Rafael G. G. Parra, Aura L. C. Goldman, Gustavo H. Oliveira, Jose T. A. Mesquita, Felipe P. Souza, Pedro F. N. Antibiotics (Basel) Article C. albicans and C. parapsilosis are biofilm-forming yeasts responsible for bloodstream infections that can cause death. Synthetic antimicrobial peptides (SAMPs) are considered to be new weapons to combat these infections, alone or combined with drugs. Here, two SAMPs, called Mo-CBP(3)-PepI and Mo-CBP(3)-PepIII, were tested alone or combined with nystatin (NYS) and itraconazole (ITR) against C. albicans and C. parapsilosis biofilms. Furthermore, the mechanism of antibiofilm activity was evaluated by fluorescence and scanning electron microscopies. When combined with SAMPs, the results revealed a 2- to 4-fold improvement of NYS and ITR antibiofilm activity. Microscopic analyses showed cell membrane and wall damage and ROS overproduction, which caused leakage of internal content and cell death. Taken together, these results suggest the potential of Mo-CBP(3)-PepI and Mo-CBP(3)-PepIII as new drugs and adjuvants to increase the activity of conventional drugs for the treatment of clinical infections caused by C. albicans and C. parapsilosis. MDPI 2022-04-21 /pmc/articles/PMC9138075/ /pubmed/35625197 http://dx.doi.org/10.3390/antibiotics11050553 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bezerra, Leandro P. Freitas, Cleverson D. T. Silva, Ayrles F. B. Amaral, Jackson L. Neto, Nilton A. S. Silva, Rafael G. G. Parra, Aura L. C. Goldman, Gustavo H. Oliveira, Jose T. A. Mesquita, Felipe P. Souza, Pedro F. N. Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms |
title | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms |
title_full | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms |
title_fullStr | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms |
title_full_unstemmed | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms |
title_short | Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms |
title_sort | synergistic antifungal activity of synthetic peptides and antifungal drugs against candida albicans and c. parapsilosis biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138075/ https://www.ncbi.nlm.nih.gov/pubmed/35625197 http://dx.doi.org/10.3390/antibiotics11050553 |
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