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Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors

Oxidative damage and inflammation are possible mechanisms linking obesity to diabetes and related complications. This study investigates the levels of oxidative damage markers in the urine of community free-living subjects with increased prevalence of obesity. Methods: Participants were assessed reg...

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Autores principales: Gariballa, Salah, Nemmar, Abderrahim, Elzaki, Ozaz, Zaaba, Nur Elena, Yasin, Javed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138160/
https://www.ncbi.nlm.nih.gov/pubmed/35624709
http://dx.doi.org/10.3390/antiox11050844
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author Gariballa, Salah
Nemmar, Abderrahim
Elzaki, Ozaz
Zaaba, Nur Elena
Yasin, Javed
author_facet Gariballa, Salah
Nemmar, Abderrahim
Elzaki, Ozaz
Zaaba, Nur Elena
Yasin, Javed
author_sort Gariballa, Salah
collection PubMed
description Oxidative damage and inflammation are possible mechanisms linking obesity to diabetes and related complications. This study investigates the levels of oxidative damage markers in the urine of community free-living subjects with increased prevalence of obesity. Methods: Participants were assessed regarding clinical, anthropometric, and physical activity data at baseline and at 6 months. Blood and urine samples were taken for the measurements of oxidative markers in urine ((glutathione (GSH), thiobarbituric acid reactive substances (TBARS), pteridine, 8-isoprostane and 8-hydroxy-2′-deoxyguanosine (8-OH-dG)), metabolic and inflammatory markers, and related biochemical variables in the blood. Univariate and multiple regression analyses were used to assess the association between oxidative markers and other clinical prognostic indicators. Results: Overall, 168 participants with a complete 6-month follow-up with a mean (±SD) age of 41 ± 12 (119 (71%) females) were included in the study. In multiple regression analysis, log-transformed urinary pteridine levels were significantly correlated with log-transformed urinary GSH, 8-isoprostane, and TBARS after adjusting for urinary creatinine at both baseline and follow-up. Significant correlations were also found between oxidative damage markers and cardiovascular disease risk factors, including systolic blood pressure, HbA1c, plasma glucose, us-C-reactive proteins, total cholesterol, and HDL. Higher TBARS levels were found in males and diabetic subjects, with lower GSH in diabetic hypertensive and obese subjects, but the latter result did not reach statistical significance. We found nonsignificantly higher TBARS, 8-isoprostane, and pteridine levels in smokers compared to those in nonsmokers. All measured urinary oxidative damage markers levels were higher in obese subjects compared with normal-weight subjects, but results did not reach statistical significance. Conclusion: we found significant associations between urinary oxidative damage and metabolic risk factors, and higher levels of urinary oxidative damage markers in diabetic, hypertensive, smoker, and male subjects.
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spelling pubmed-91381602022-05-28 Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors Gariballa, Salah Nemmar, Abderrahim Elzaki, Ozaz Zaaba, Nur Elena Yasin, Javed Antioxidants (Basel) Article Oxidative damage and inflammation are possible mechanisms linking obesity to diabetes and related complications. This study investigates the levels of oxidative damage markers in the urine of community free-living subjects with increased prevalence of obesity. Methods: Participants were assessed regarding clinical, anthropometric, and physical activity data at baseline and at 6 months. Blood and urine samples were taken for the measurements of oxidative markers in urine ((glutathione (GSH), thiobarbituric acid reactive substances (TBARS), pteridine, 8-isoprostane and 8-hydroxy-2′-deoxyguanosine (8-OH-dG)), metabolic and inflammatory markers, and related biochemical variables in the blood. Univariate and multiple regression analyses were used to assess the association between oxidative markers and other clinical prognostic indicators. Results: Overall, 168 participants with a complete 6-month follow-up with a mean (±SD) age of 41 ± 12 (119 (71%) females) were included in the study. In multiple regression analysis, log-transformed urinary pteridine levels were significantly correlated with log-transformed urinary GSH, 8-isoprostane, and TBARS after adjusting for urinary creatinine at both baseline and follow-up. Significant correlations were also found between oxidative damage markers and cardiovascular disease risk factors, including systolic blood pressure, HbA1c, plasma glucose, us-C-reactive proteins, total cholesterol, and HDL. Higher TBARS levels were found in males and diabetic subjects, with lower GSH in diabetic hypertensive and obese subjects, but the latter result did not reach statistical significance. We found nonsignificantly higher TBARS, 8-isoprostane, and pteridine levels in smokers compared to those in nonsmokers. All measured urinary oxidative damage markers levels were higher in obese subjects compared with normal-weight subjects, but results did not reach statistical significance. Conclusion: we found significant associations between urinary oxidative damage and metabolic risk factors, and higher levels of urinary oxidative damage markers in diabetic, hypertensive, smoker, and male subjects. MDPI 2022-04-26 /pmc/articles/PMC9138160/ /pubmed/35624709 http://dx.doi.org/10.3390/antiox11050844 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gariballa, Salah
Nemmar, Abderrahim
Elzaki, Ozaz
Zaaba, Nur Elena
Yasin, Javed
Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors
title Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors
title_full Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors
title_fullStr Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors
title_full_unstemmed Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors
title_short Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors
title_sort urinary oxidative damage markers and their association with obesity-related metabolic risk factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138160/
https://www.ncbi.nlm.nih.gov/pubmed/35624709
http://dx.doi.org/10.3390/antiox11050844
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