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Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction

Acute myocardial infarction (AMI) is a manifestation of ischemic heart disease where the immune system plays an important role in the re-establishment of homeostasis. We hypothesize that the anti-inflammatory activity of secretomes from menstrual blood-derived mesenchymal stromal cells (S-MenSCs) an...

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Autores principales: de Pedro, María Ángeles, Pulido, María, Marinaro, Federica, Álvarez, Verónica, Báez-Díaz, Claudia, Blanco, Virginia, Silla-Castro, Juan Carlos, Sanchez-Cabo, Fátima, Sánchez-Margallo, Francisco Miguel, Crisóstomo, Verónica, Casado, Javier G., López, Esther
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138214/
https://www.ncbi.nlm.nih.gov/pubmed/35625854
http://dx.doi.org/10.3390/biomedicines10051117
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author de Pedro, María Ángeles
Pulido, María
Marinaro, Federica
Álvarez, Verónica
Báez-Díaz, Claudia
Blanco, Virginia
Silla-Castro, Juan Carlos
Sanchez-Cabo, Fátima
Sánchez-Margallo, Francisco Miguel
Crisóstomo, Verónica
Casado, Javier G.
López, Esther
author_facet de Pedro, María Ángeles
Pulido, María
Marinaro, Federica
Álvarez, Verónica
Báez-Díaz, Claudia
Blanco, Virginia
Silla-Castro, Juan Carlos
Sanchez-Cabo, Fátima
Sánchez-Margallo, Francisco Miguel
Crisóstomo, Verónica
Casado, Javier G.
López, Esther
author_sort de Pedro, María Ángeles
collection PubMed
description Acute myocardial infarction (AMI) is a manifestation of ischemic heart disease where the immune system plays an important role in the re-establishment of homeostasis. We hypothesize that the anti-inflammatory activity of secretomes from menstrual blood-derived mesenchymal stromal cells (S-MenSCs) and IFNγ/TNFα-primed MenSCs (S-MenSCs*) may be considered a therapeutic option for the treatment of AMI. To assess this hypothesis, we have evaluated the effect of S-MenSCs and S-MenSCs* on cardiac function parameters and the involvement of immune-related genes using a porcine model of AMI. Twelve pigs were randomly divided into three biogroups: AMI/Placebo, AMI/S-MenSCs, and AMI/S-MenSCs*. AMI models were generated using a closed chest coronary occlusion-reperfusion procedure and, after 72 h, the different treatments were intrapericardially administered. Cardiac function parameters were monitored by magnetic resonance imaging before and 7 days post-therapy. Transcriptomic analyses in the infarcted tissue identified 571 transcripts associated with the Gene Ontology term Immune response, of which 57 were differentially expressed when different biogroups were compared. Moreover, a prediction of the interactions between differentially expressed genes (DEGs) and miRNAs from secretomes revealed that some DEGs in the infarction area, such as STAT3, IGFR1, or BCL6 could be targeted by previously identified miRNAs in secretomes from MenSCs. In conclusion, the intrapericardial administration of secretome early after infarction has a significant impact on the expression of immune-related genes in the infarcted myocardium. This confirms the immunomodulatory potential of intrapericardially delivered secretomes and opens new therapeutic perspectives in myocardial infarction treatment.
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spelling pubmed-91382142022-05-28 Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction de Pedro, María Ángeles Pulido, María Marinaro, Federica Álvarez, Verónica Báez-Díaz, Claudia Blanco, Virginia Silla-Castro, Juan Carlos Sanchez-Cabo, Fátima Sánchez-Margallo, Francisco Miguel Crisóstomo, Verónica Casado, Javier G. López, Esther Biomedicines Article Acute myocardial infarction (AMI) is a manifestation of ischemic heart disease where the immune system plays an important role in the re-establishment of homeostasis. We hypothesize that the anti-inflammatory activity of secretomes from menstrual blood-derived mesenchymal stromal cells (S-MenSCs) and IFNγ/TNFα-primed MenSCs (S-MenSCs*) may be considered a therapeutic option for the treatment of AMI. To assess this hypothesis, we have evaluated the effect of S-MenSCs and S-MenSCs* on cardiac function parameters and the involvement of immune-related genes using a porcine model of AMI. Twelve pigs were randomly divided into three biogroups: AMI/Placebo, AMI/S-MenSCs, and AMI/S-MenSCs*. AMI models were generated using a closed chest coronary occlusion-reperfusion procedure and, after 72 h, the different treatments were intrapericardially administered. Cardiac function parameters were monitored by magnetic resonance imaging before and 7 days post-therapy. Transcriptomic analyses in the infarcted tissue identified 571 transcripts associated with the Gene Ontology term Immune response, of which 57 were differentially expressed when different biogroups were compared. Moreover, a prediction of the interactions between differentially expressed genes (DEGs) and miRNAs from secretomes revealed that some DEGs in the infarction area, such as STAT3, IGFR1, or BCL6 could be targeted by previously identified miRNAs in secretomes from MenSCs. In conclusion, the intrapericardial administration of secretome early after infarction has a significant impact on the expression of immune-related genes in the infarcted myocardium. This confirms the immunomodulatory potential of intrapericardially delivered secretomes and opens new therapeutic perspectives in myocardial infarction treatment. MDPI 2022-05-11 /pmc/articles/PMC9138214/ /pubmed/35625854 http://dx.doi.org/10.3390/biomedicines10051117 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Pedro, María Ángeles
Pulido, María
Marinaro, Federica
Álvarez, Verónica
Báez-Díaz, Claudia
Blanco, Virginia
Silla-Castro, Juan Carlos
Sanchez-Cabo, Fátima
Sánchez-Margallo, Francisco Miguel
Crisóstomo, Verónica
Casado, Javier G.
López, Esther
Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction
title Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction
title_full Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction
title_fullStr Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction
title_full_unstemmed Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction
title_short Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction
title_sort intrapericardial administration of secretomes from menstrual blood-derived mesenchymal stromal cells: effects on immune-related genes in a porcine model of myocardial infarction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138214/
https://www.ncbi.nlm.nih.gov/pubmed/35625854
http://dx.doi.org/10.3390/biomedicines10051117
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