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Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors

In recent years, the application of cell-free protein synthesis systems in biosensing has been developing rapidly. Cell-free synthetic biology, with its advantages of high biosafety, fast material transport, and high sensitivity, has overcome many defects of cell-based biosensors and provided an abi...

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Detalles Bibliográficos
Autores principales: Wang, Ting, Lu, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138237/
https://www.ncbi.nlm.nih.gov/pubmed/35624619
http://dx.doi.org/10.3390/bios12050318
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author Wang, Ting
Lu, Yuan
author_facet Wang, Ting
Lu, Yuan
author_sort Wang, Ting
collection PubMed
description In recent years, the application of cell-free protein synthesis systems in biosensing has been developing rapidly. Cell-free synthetic biology, with its advantages of high biosafety, fast material transport, and high sensitivity, has overcome many defects of cell-based biosensors and provided an abiotic substitute for biosensors. In addition, the application of freeze-drying technology has improved the stability of such systems, making it possible to realize point-of-care application of field detection and broadening the application prospects of cell-free biosensors. However, despite these advancements, challenges such as the risk of sample interference due to the lack of physical barriers, maintenance of activity during storage, and poor robustness still need to be addressed before the full potential of cell-free biosensors can be realized on a larger scale. In this review, current strategies and research results for improving the performance of cell-free biosensors are summarized, including a comprehensive discussion of the existing challenges, future trends, and potential investments needed for improvement.
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spelling pubmed-91382372022-05-28 Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors Wang, Ting Lu, Yuan Biosensors (Basel) Perspective In recent years, the application of cell-free protein synthesis systems in biosensing has been developing rapidly. Cell-free synthetic biology, with its advantages of high biosafety, fast material transport, and high sensitivity, has overcome many defects of cell-based biosensors and provided an abiotic substitute for biosensors. In addition, the application of freeze-drying technology has improved the stability of such systems, making it possible to realize point-of-care application of field detection and broadening the application prospects of cell-free biosensors. However, despite these advancements, challenges such as the risk of sample interference due to the lack of physical barriers, maintenance of activity during storage, and poor robustness still need to be addressed before the full potential of cell-free biosensors can be realized on a larger scale. In this review, current strategies and research results for improving the performance of cell-free biosensors are summarized, including a comprehensive discussion of the existing challenges, future trends, and potential investments needed for improvement. MDPI 2022-05-10 /pmc/articles/PMC9138237/ /pubmed/35624619 http://dx.doi.org/10.3390/bios12050318 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Perspective
Wang, Ting
Lu, Yuan
Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors
title Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors
title_full Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors
title_fullStr Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors
title_full_unstemmed Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors
title_short Advances, Challenges and Future Trends of Cell-Free Transcription-Translation Biosensors
title_sort advances, challenges and future trends of cell-free transcription-translation biosensors
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138237/
https://www.ncbi.nlm.nih.gov/pubmed/35624619
http://dx.doi.org/10.3390/bios12050318
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