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Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli
As bulk chemicals, diols have wide applications in many fields, such as clothing, biofuels, food, surfactant and cosmetics. The traditional chemical synthesis of diols consumes numerous non-renewable energy resources and leads to environmental pollution. Green biosynthesis has emerged as an alternat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138338/ https://www.ncbi.nlm.nih.gov/pubmed/35625642 http://dx.doi.org/10.3390/biom12050715 |
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author | Wu, Tong Liu, Yumei Liu, Jinsheng Chen, Zhenya Huo, Yi-Xin |
author_facet | Wu, Tong Liu, Yumei Liu, Jinsheng Chen, Zhenya Huo, Yi-Xin |
author_sort | Wu, Tong |
collection | PubMed |
description | As bulk chemicals, diols have wide applications in many fields, such as clothing, biofuels, food, surfactant and cosmetics. The traditional chemical synthesis of diols consumes numerous non-renewable energy resources and leads to environmental pollution. Green biosynthesis has emerged as an alternative method to produce diols. Escherichia coli as an ideal microbial factory has been engineered to biosynthesize diols from carbon sources. Here, we comprehensively summarized the biosynthetic pathways of diols from renewable biomass in E. coli and discussed the metabolic-engineering strategies that could enhance the production of diols, including the optimization of biosynthetic pathways, improvement of cofactor supplementation, and reprogramming of the metabolic network. We then investigated the dynamic regulation by multiple control modules to balance the growth and production, so as to direct carbon sources for diol production. Finally, we proposed the challenges in the diol-biosynthesis process and suggested some potential methods to improve the diol-producing ability of the host. |
format | Online Article Text |
id | pubmed-9138338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91383382022-05-28 Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli Wu, Tong Liu, Yumei Liu, Jinsheng Chen, Zhenya Huo, Yi-Xin Biomolecules Review As bulk chemicals, diols have wide applications in many fields, such as clothing, biofuels, food, surfactant and cosmetics. The traditional chemical synthesis of diols consumes numerous non-renewable energy resources and leads to environmental pollution. Green biosynthesis has emerged as an alternative method to produce diols. Escherichia coli as an ideal microbial factory has been engineered to biosynthesize diols from carbon sources. Here, we comprehensively summarized the biosynthetic pathways of diols from renewable biomass in E. coli and discussed the metabolic-engineering strategies that could enhance the production of diols, including the optimization of biosynthetic pathways, improvement of cofactor supplementation, and reprogramming of the metabolic network. We then investigated the dynamic regulation by multiple control modules to balance the growth and production, so as to direct carbon sources for diol production. Finally, we proposed the challenges in the diol-biosynthesis process and suggested some potential methods to improve the diol-producing ability of the host. MDPI 2022-05-18 /pmc/articles/PMC9138338/ /pubmed/35625642 http://dx.doi.org/10.3390/biom12050715 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Wu, Tong Liu, Yumei Liu, Jinsheng Chen, Zhenya Huo, Yi-Xin Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli |
title | Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli |
title_full | Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli |
title_fullStr | Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli |
title_full_unstemmed | Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli |
title_short | Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli |
title_sort | metabolic engineering and regulation of diol biosynthesis from renewable biomass in escherichia coli |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138338/ https://www.ncbi.nlm.nih.gov/pubmed/35625642 http://dx.doi.org/10.3390/biom12050715 |
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