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Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli

As bulk chemicals, diols have wide applications in many fields, such as clothing, biofuels, food, surfactant and cosmetics. The traditional chemical synthesis of diols consumes numerous non-renewable energy resources and leads to environmental pollution. Green biosynthesis has emerged as an alternat...

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Autores principales: Wu, Tong, Liu, Yumei, Liu, Jinsheng, Chen, Zhenya, Huo, Yi-Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138338/
https://www.ncbi.nlm.nih.gov/pubmed/35625642
http://dx.doi.org/10.3390/biom12050715
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author Wu, Tong
Liu, Yumei
Liu, Jinsheng
Chen, Zhenya
Huo, Yi-Xin
author_facet Wu, Tong
Liu, Yumei
Liu, Jinsheng
Chen, Zhenya
Huo, Yi-Xin
author_sort Wu, Tong
collection PubMed
description As bulk chemicals, diols have wide applications in many fields, such as clothing, biofuels, food, surfactant and cosmetics. The traditional chemical synthesis of diols consumes numerous non-renewable energy resources and leads to environmental pollution. Green biosynthesis has emerged as an alternative method to produce diols. Escherichia coli as an ideal microbial factory has been engineered to biosynthesize diols from carbon sources. Here, we comprehensively summarized the biosynthetic pathways of diols from renewable biomass in E. coli and discussed the metabolic-engineering strategies that could enhance the production of diols, including the optimization of biosynthetic pathways, improvement of cofactor supplementation, and reprogramming of the metabolic network. We then investigated the dynamic regulation by multiple control modules to balance the growth and production, so as to direct carbon sources for diol production. Finally, we proposed the challenges in the diol-biosynthesis process and suggested some potential methods to improve the diol-producing ability of the host.
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spelling pubmed-91383382022-05-28 Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli Wu, Tong Liu, Yumei Liu, Jinsheng Chen, Zhenya Huo, Yi-Xin Biomolecules Review As bulk chemicals, diols have wide applications in many fields, such as clothing, biofuels, food, surfactant and cosmetics. The traditional chemical synthesis of diols consumes numerous non-renewable energy resources and leads to environmental pollution. Green biosynthesis has emerged as an alternative method to produce diols. Escherichia coli as an ideal microbial factory has been engineered to biosynthesize diols from carbon sources. Here, we comprehensively summarized the biosynthetic pathways of diols from renewable biomass in E. coli and discussed the metabolic-engineering strategies that could enhance the production of diols, including the optimization of biosynthetic pathways, improvement of cofactor supplementation, and reprogramming of the metabolic network. We then investigated the dynamic regulation by multiple control modules to balance the growth and production, so as to direct carbon sources for diol production. Finally, we proposed the challenges in the diol-biosynthesis process and suggested some potential methods to improve the diol-producing ability of the host. MDPI 2022-05-18 /pmc/articles/PMC9138338/ /pubmed/35625642 http://dx.doi.org/10.3390/biom12050715 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Wu, Tong
Liu, Yumei
Liu, Jinsheng
Chen, Zhenya
Huo, Yi-Xin
Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli
title Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli
title_full Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli
title_fullStr Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli
title_full_unstemmed Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli
title_short Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli
title_sort metabolic engineering and regulation of diol biosynthesis from renewable biomass in escherichia coli
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138338/
https://www.ncbi.nlm.nih.gov/pubmed/35625642
http://dx.doi.org/10.3390/biom12050715
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