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Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction

Lysosomes are ubiquitous membrane-bound organelles found in all eukaryotic cells. Outside of their well-known degradative function, lysosomes are integral in maintaining cellular homeostasis. Growing evidence has shown that lysosomal dysfunction plays an important role not only in the rare group of...

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Autores principales: Chen, Chase, Sidransky, Ellen, Chen, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138597/
https://www.ncbi.nlm.nih.gov/pubmed/35625544
http://dx.doi.org/10.3390/biom12050616
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author Chen, Chase
Sidransky, Ellen
Chen, Yu
author_facet Chen, Chase
Sidransky, Ellen
Chen, Yu
author_sort Chen, Chase
collection PubMed
description Lysosomes are ubiquitous membrane-bound organelles found in all eukaryotic cells. Outside of their well-known degradative function, lysosomes are integral in maintaining cellular homeostasis. Growing evidence has shown that lysosomal dysfunction plays an important role not only in the rare group of lysosomal storage diseases but also in a host of others, including common neurodegenerative disorders, such as Alzheimer disease and Parkinson disease. New technological advances have significantly increased our ability to rapidly isolate lysosomes from cells in recent years. The development of the Lyso-IP approach and similar methods now allow for lysosomal purification within ten minutes. Multiple studies using the Lyso-IP approach have revealed novel insights into the pathogenic mechanisms of lysosomal disorders, including Niemann-Pick type C disease, showing the immense potential for this technique. Future applications of rapid lysosomal isolation techniques are likely to greatly enhance our understanding of lysosomal dysfunction in rare and common neurodegeneration causes.
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spelling pubmed-91385972022-05-28 Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction Chen, Chase Sidransky, Ellen Chen, Yu Biomolecules Review Lysosomes are ubiquitous membrane-bound organelles found in all eukaryotic cells. Outside of their well-known degradative function, lysosomes are integral in maintaining cellular homeostasis. Growing evidence has shown that lysosomal dysfunction plays an important role not only in the rare group of lysosomal storage diseases but also in a host of others, including common neurodegenerative disorders, such as Alzheimer disease and Parkinson disease. New technological advances have significantly increased our ability to rapidly isolate lysosomes from cells in recent years. The development of the Lyso-IP approach and similar methods now allow for lysosomal purification within ten minutes. Multiple studies using the Lyso-IP approach have revealed novel insights into the pathogenic mechanisms of lysosomal disorders, including Niemann-Pick type C disease, showing the immense potential for this technique. Future applications of rapid lysosomal isolation techniques are likely to greatly enhance our understanding of lysosomal dysfunction in rare and common neurodegeneration causes. MDPI 2022-04-21 /pmc/articles/PMC9138597/ /pubmed/35625544 http://dx.doi.org/10.3390/biom12050616 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Chen, Chase
Sidransky, Ellen
Chen, Yu
Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction
title Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction
title_full Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction
title_fullStr Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction
title_full_unstemmed Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction
title_short Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction
title_sort lyso-ip: uncovering pathogenic mechanisms of lysosomal dysfunction
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138597/
https://www.ncbi.nlm.nih.gov/pubmed/35625544
http://dx.doi.org/10.3390/biom12050616
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