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ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates
Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migrat...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138674/ https://www.ncbi.nlm.nih.gov/pubmed/35624596 http://dx.doi.org/10.3390/bios12050293 |
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author | Hung, Yu-Han Chiu, Wei-Chih Fuh, Shyh-Rong Lai, Yi-Ting Tung, Tse-Hua Huang, Chun-Chung Lo, Chun-Min |
author_facet | Hung, Yu-Han Chiu, Wei-Chih Fuh, Shyh-Rong Lai, Yi-Ting Tung, Tse-Hua Huang, Chun-Chung Lo, Chun-Min |
author_sort | Hung, Yu-Han |
collection | PubMed |
description | Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migration. In this study, we applied ECIS electric fence (EF) method, an alternative to electrical wounding, to assess the effects of different surface coatings on human keratinocyte (HaCaT) migration. The EF prevents inoculated cells from attaching or migrating to the fenced electrode surface while maintaining the integrity of the surface coating. After the EF is turned off, cells migrate into the cell-free area, and the increase in measured impedance is monitored. We cultured HaCaT cells on gold electrodes without coating or coated with poly-L-lysin (PLL), poly-D-lysine (PDL), or type-I collagen. We quantified migration rates according to the different slopes in the impedance time series. It was observed that either poly-L-lysine (PLL) or poly-D-lysine (PDL) limits cell adhesion and migration rates. Furthermore, the surface charge of the coated substrate in the culture condition positively correlates with the cell adhesion and migration process. Our results indicate that the EF method is useful for determining cell migration rates on specific surface coatings. |
format | Online Article Text |
id | pubmed-9138674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91386742022-05-28 ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates Hung, Yu-Han Chiu, Wei-Chih Fuh, Shyh-Rong Lai, Yi-Ting Tung, Tse-Hua Huang, Chun-Chung Lo, Chun-Min Biosensors (Basel) Article Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migration. In this study, we applied ECIS electric fence (EF) method, an alternative to electrical wounding, to assess the effects of different surface coatings on human keratinocyte (HaCaT) migration. The EF prevents inoculated cells from attaching or migrating to the fenced electrode surface while maintaining the integrity of the surface coating. After the EF is turned off, cells migrate into the cell-free area, and the increase in measured impedance is monitored. We cultured HaCaT cells on gold electrodes without coating or coated with poly-L-lysin (PLL), poly-D-lysine (PDL), or type-I collagen. We quantified migration rates according to the different slopes in the impedance time series. It was observed that either poly-L-lysine (PLL) or poly-D-lysine (PDL) limits cell adhesion and migration rates. Furthermore, the surface charge of the coated substrate in the culture condition positively correlates with the cell adhesion and migration process. Our results indicate that the EF method is useful for determining cell migration rates on specific surface coatings. MDPI 2022-05-03 /pmc/articles/PMC9138674/ /pubmed/35624596 http://dx.doi.org/10.3390/bios12050293 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hung, Yu-Han Chiu, Wei-Chih Fuh, Shyh-Rong Lai, Yi-Ting Tung, Tse-Hua Huang, Chun-Chung Lo, Chun-Min ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
title | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
title_full | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
title_fullStr | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
title_full_unstemmed | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
title_short | ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates |
title_sort | ecis based electric fence method for measurement of human keratinocyte migration on different substrates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138674/ https://www.ncbi.nlm.nih.gov/pubmed/35624596 http://dx.doi.org/10.3390/bios12050293 |
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