Cargando…

ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates

Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migrat...

Descripción completa

Detalles Bibliográficos
Autores principales: Hung, Yu-Han, Chiu, Wei-Chih, Fuh, Shyh-Rong, Lai, Yi-Ting, Tung, Tse-Hua, Huang, Chun-Chung, Lo, Chun-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138674/
https://www.ncbi.nlm.nih.gov/pubmed/35624596
http://dx.doi.org/10.3390/bios12050293
_version_ 1784714679213359104
author Hung, Yu-Han
Chiu, Wei-Chih
Fuh, Shyh-Rong
Lai, Yi-Ting
Tung, Tse-Hua
Huang, Chun-Chung
Lo, Chun-Min
author_facet Hung, Yu-Han
Chiu, Wei-Chih
Fuh, Shyh-Rong
Lai, Yi-Ting
Tung, Tse-Hua
Huang, Chun-Chung
Lo, Chun-Min
author_sort Hung, Yu-Han
collection PubMed
description Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migration. In this study, we applied ECIS electric fence (EF) method, an alternative to electrical wounding, to assess the effects of different surface coatings on human keratinocyte (HaCaT) migration. The EF prevents inoculated cells from attaching or migrating to the fenced electrode surface while maintaining the integrity of the surface coating. After the EF is turned off, cells migrate into the cell-free area, and the increase in measured impedance is monitored. We cultured HaCaT cells on gold electrodes without coating or coated with poly-L-lysin (PLL), poly-D-lysine (PDL), or type-I collagen. We quantified migration rates according to the different slopes in the impedance time series. It was observed that either poly-L-lysine (PLL) or poly-D-lysine (PDL) limits cell adhesion and migration rates. Furthermore, the surface charge of the coated substrate in the culture condition positively correlates with the cell adhesion and migration process. Our results indicate that the EF method is useful for determining cell migration rates on specific surface coatings.
format Online
Article
Text
id pubmed-9138674
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91386742022-05-28 ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates Hung, Yu-Han Chiu, Wei-Chih Fuh, Shyh-Rong Lai, Yi-Ting Tung, Tse-Hua Huang, Chun-Chung Lo, Chun-Min Biosensors (Basel) Article Electric Cell-substrate Impedance Sensing (ECIS) is an impedance-based, real-time, and label-free measuring system for monitoring cellular activities in tissue culture. Previously, ECIS wound healing assay has been used to wound cells with high electric current and monitor the subsequent cell migration. In this study, we applied ECIS electric fence (EF) method, an alternative to electrical wounding, to assess the effects of different surface coatings on human keratinocyte (HaCaT) migration. The EF prevents inoculated cells from attaching or migrating to the fenced electrode surface while maintaining the integrity of the surface coating. After the EF is turned off, cells migrate into the cell-free area, and the increase in measured impedance is monitored. We cultured HaCaT cells on gold electrodes without coating or coated with poly-L-lysin (PLL), poly-D-lysine (PDL), or type-I collagen. We quantified migration rates according to the different slopes in the impedance time series. It was observed that either poly-L-lysine (PLL) or poly-D-lysine (PDL) limits cell adhesion and migration rates. Furthermore, the surface charge of the coated substrate in the culture condition positively correlates with the cell adhesion and migration process. Our results indicate that the EF method is useful for determining cell migration rates on specific surface coatings. MDPI 2022-05-03 /pmc/articles/PMC9138674/ /pubmed/35624596 http://dx.doi.org/10.3390/bios12050293 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hung, Yu-Han
Chiu, Wei-Chih
Fuh, Shyh-Rong
Lai, Yi-Ting
Tung, Tse-Hua
Huang, Chun-Chung
Lo, Chun-Min
ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates
title ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates
title_full ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates
title_fullStr ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates
title_full_unstemmed ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates
title_short ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates
title_sort ecis based electric fence method for measurement of human keratinocyte migration on different substrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138674/
https://www.ncbi.nlm.nih.gov/pubmed/35624596
http://dx.doi.org/10.3390/bios12050293
work_keys_str_mv AT hungyuhan ecisbasedelectricfencemethodformeasurementofhumankeratinocytemigrationondifferentsubstrates
AT chiuweichih ecisbasedelectricfencemethodformeasurementofhumankeratinocytemigrationondifferentsubstrates
AT fuhshyhrong ecisbasedelectricfencemethodformeasurementofhumankeratinocytemigrationondifferentsubstrates
AT laiyiting ecisbasedelectricfencemethodformeasurementofhumankeratinocytemigrationondifferentsubstrates
AT tungtsehua ecisbasedelectricfencemethodformeasurementofhumankeratinocytemigrationondifferentsubstrates
AT huangchunchung ecisbasedelectricfencemethodformeasurementofhumankeratinocytemigrationondifferentsubstrates
AT lochunmin ecisbasedelectricfencemethodformeasurementofhumankeratinocytemigrationondifferentsubstrates