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Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma
Juvenile angiofibroma (JA) is a rare fibrovascular neoplasm predominately found within the posterior nasal cavity of adolescent males. JA expresses the proteoglycan nerve–glial antigen (NG)2, which crucially determines the migratory capacity of distinct cancer cells. Moreover, it is known that the p...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138789/ https://www.ncbi.nlm.nih.gov/pubmed/35625703 http://dx.doi.org/10.3390/biomedicines10050966 |
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author | Boewe, Anne S. Wemmert, Silke Kulas, Philipp Schick, Bernhard Götz, Claudia Wrublewsky, Selina Montenarh, Mathias Menger, Michael D. Laschke, Matthias W. Ampofo, Emmanuel |
author_facet | Boewe, Anne S. Wemmert, Silke Kulas, Philipp Schick, Bernhard Götz, Claudia Wrublewsky, Selina Montenarh, Mathias Menger, Michael D. Laschke, Matthias W. Ampofo, Emmanuel |
author_sort | Boewe, Anne S. |
collection | PubMed |
description | Juvenile angiofibroma (JA) is a rare fibrovascular neoplasm predominately found within the posterior nasal cavity of adolescent males. JA expresses the proteoglycan nerve–glial antigen (NG)2, which crucially determines the migratory capacity of distinct cancer cells. Moreover, it is known that the protein kinase CK2 regulates NG2 gene expression. Therefore, in the present study, we analyzed whether the inhibition of CK2 suppresses NG2-dependent JA cell proliferation and migration. For this purpose, we assessed the expression of NG2 and CK2 in patient-derived JA tissue samples, as well as in patient-derived JA cell cultures by Western blot, immunohistochemistry, flow cytometry and quantitative real-time PCR. The mitochondrial activity, proliferation and migratory capacity of the JA cells were determined by water-soluble tetrazolium (WST)-1, 5-bromo-2′-deoxyuridine (BrdU) and collagen sprouting assays. We found that NG2 and CK2 were expressed in both the JA tissue samples and cell cultures. The treatment of the JA cells with the two CK2 inhibitors, CX-4945 and SGC-CK2-1, significantly reduced NG2 gene and protein expression when compared to the vehicle-treated cells. In addition, the loss of CK2 activity suppressed the JA cell proliferation and migration. These findings indicate that the inhibition of CK2 may represent a promising therapeutic approach for the treatment of NG2-expressing JA. |
format | Online Article Text |
id | pubmed-9138789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91387892022-05-28 Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma Boewe, Anne S. Wemmert, Silke Kulas, Philipp Schick, Bernhard Götz, Claudia Wrublewsky, Selina Montenarh, Mathias Menger, Michael D. Laschke, Matthias W. Ampofo, Emmanuel Biomedicines Article Juvenile angiofibroma (JA) is a rare fibrovascular neoplasm predominately found within the posterior nasal cavity of adolescent males. JA expresses the proteoglycan nerve–glial antigen (NG)2, which crucially determines the migratory capacity of distinct cancer cells. Moreover, it is known that the protein kinase CK2 regulates NG2 gene expression. Therefore, in the present study, we analyzed whether the inhibition of CK2 suppresses NG2-dependent JA cell proliferation and migration. For this purpose, we assessed the expression of NG2 and CK2 in patient-derived JA tissue samples, as well as in patient-derived JA cell cultures by Western blot, immunohistochemistry, flow cytometry and quantitative real-time PCR. The mitochondrial activity, proliferation and migratory capacity of the JA cells were determined by water-soluble tetrazolium (WST)-1, 5-bromo-2′-deoxyuridine (BrdU) and collagen sprouting assays. We found that NG2 and CK2 were expressed in both the JA tissue samples and cell cultures. The treatment of the JA cells with the two CK2 inhibitors, CX-4945 and SGC-CK2-1, significantly reduced NG2 gene and protein expression when compared to the vehicle-treated cells. In addition, the loss of CK2 activity suppressed the JA cell proliferation and migration. These findings indicate that the inhibition of CK2 may represent a promising therapeutic approach for the treatment of NG2-expressing JA. MDPI 2022-04-21 /pmc/articles/PMC9138789/ /pubmed/35625703 http://dx.doi.org/10.3390/biomedicines10050966 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Boewe, Anne S. Wemmert, Silke Kulas, Philipp Schick, Bernhard Götz, Claudia Wrublewsky, Selina Montenarh, Mathias Menger, Michael D. Laschke, Matthias W. Ampofo, Emmanuel Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma |
title | Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma |
title_full | Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma |
title_fullStr | Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma |
title_full_unstemmed | Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma |
title_short | Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma |
title_sort | inhibition of ck2 reduces ng2 expression in juvenile angiofibroma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138789/ https://www.ncbi.nlm.nih.gov/pubmed/35625703 http://dx.doi.org/10.3390/biomedicines10050966 |
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