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Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma

Juvenile angiofibroma (JA) is a rare fibrovascular neoplasm predominately found within the posterior nasal cavity of adolescent males. JA expresses the proteoglycan nerve–glial antigen (NG)2, which crucially determines the migratory capacity of distinct cancer cells. Moreover, it is known that the p...

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Autores principales: Boewe, Anne S., Wemmert, Silke, Kulas, Philipp, Schick, Bernhard, Götz, Claudia, Wrublewsky, Selina, Montenarh, Mathias, Menger, Michael D., Laschke, Matthias W., Ampofo, Emmanuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138789/
https://www.ncbi.nlm.nih.gov/pubmed/35625703
http://dx.doi.org/10.3390/biomedicines10050966
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author Boewe, Anne S.
Wemmert, Silke
Kulas, Philipp
Schick, Bernhard
Götz, Claudia
Wrublewsky, Selina
Montenarh, Mathias
Menger, Michael D.
Laschke, Matthias W.
Ampofo, Emmanuel
author_facet Boewe, Anne S.
Wemmert, Silke
Kulas, Philipp
Schick, Bernhard
Götz, Claudia
Wrublewsky, Selina
Montenarh, Mathias
Menger, Michael D.
Laschke, Matthias W.
Ampofo, Emmanuel
author_sort Boewe, Anne S.
collection PubMed
description Juvenile angiofibroma (JA) is a rare fibrovascular neoplasm predominately found within the posterior nasal cavity of adolescent males. JA expresses the proteoglycan nerve–glial antigen (NG)2, which crucially determines the migratory capacity of distinct cancer cells. Moreover, it is known that the protein kinase CK2 regulates NG2 gene expression. Therefore, in the present study, we analyzed whether the inhibition of CK2 suppresses NG2-dependent JA cell proliferation and migration. For this purpose, we assessed the expression of NG2 and CK2 in patient-derived JA tissue samples, as well as in patient-derived JA cell cultures by Western blot, immunohistochemistry, flow cytometry and quantitative real-time PCR. The mitochondrial activity, proliferation and migratory capacity of the JA cells were determined by water-soluble tetrazolium (WST)-1, 5-bromo-2′-deoxyuridine (BrdU) and collagen sprouting assays. We found that NG2 and CK2 were expressed in both the JA tissue samples and cell cultures. The treatment of the JA cells with the two CK2 inhibitors, CX-4945 and SGC-CK2-1, significantly reduced NG2 gene and protein expression when compared to the vehicle-treated cells. In addition, the loss of CK2 activity suppressed the JA cell proliferation and migration. These findings indicate that the inhibition of CK2 may represent a promising therapeutic approach for the treatment of NG2-expressing JA.
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spelling pubmed-91387892022-05-28 Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma Boewe, Anne S. Wemmert, Silke Kulas, Philipp Schick, Bernhard Götz, Claudia Wrublewsky, Selina Montenarh, Mathias Menger, Michael D. Laschke, Matthias W. Ampofo, Emmanuel Biomedicines Article Juvenile angiofibroma (JA) is a rare fibrovascular neoplasm predominately found within the posterior nasal cavity of adolescent males. JA expresses the proteoglycan nerve–glial antigen (NG)2, which crucially determines the migratory capacity of distinct cancer cells. Moreover, it is known that the protein kinase CK2 regulates NG2 gene expression. Therefore, in the present study, we analyzed whether the inhibition of CK2 suppresses NG2-dependent JA cell proliferation and migration. For this purpose, we assessed the expression of NG2 and CK2 in patient-derived JA tissue samples, as well as in patient-derived JA cell cultures by Western blot, immunohistochemistry, flow cytometry and quantitative real-time PCR. The mitochondrial activity, proliferation and migratory capacity of the JA cells were determined by water-soluble tetrazolium (WST)-1, 5-bromo-2′-deoxyuridine (BrdU) and collagen sprouting assays. We found that NG2 and CK2 were expressed in both the JA tissue samples and cell cultures. The treatment of the JA cells with the two CK2 inhibitors, CX-4945 and SGC-CK2-1, significantly reduced NG2 gene and protein expression when compared to the vehicle-treated cells. In addition, the loss of CK2 activity suppressed the JA cell proliferation and migration. These findings indicate that the inhibition of CK2 may represent a promising therapeutic approach for the treatment of NG2-expressing JA. MDPI 2022-04-21 /pmc/articles/PMC9138789/ /pubmed/35625703 http://dx.doi.org/10.3390/biomedicines10050966 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boewe, Anne S.
Wemmert, Silke
Kulas, Philipp
Schick, Bernhard
Götz, Claudia
Wrublewsky, Selina
Montenarh, Mathias
Menger, Michael D.
Laschke, Matthias W.
Ampofo, Emmanuel
Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma
title Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma
title_full Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma
title_fullStr Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma
title_full_unstemmed Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma
title_short Inhibition of CK2 Reduces NG2 Expression in Juvenile Angiofibroma
title_sort inhibition of ck2 reduces ng2 expression in juvenile angiofibroma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138789/
https://www.ncbi.nlm.nih.gov/pubmed/35625703
http://dx.doi.org/10.3390/biomedicines10050966
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