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Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range
Suspended particles play a vital role in aquatic environments. We propose a method to rapidly measure the scattered polarization parameters of individual suspended particles with continuously large angular range (PCLAR), from 60° to 120° in one shot. A conceptual setup is built to measure PCLAR with...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138884/ https://www.ncbi.nlm.nih.gov/pubmed/35624622 http://dx.doi.org/10.3390/bios12050321 |
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author | Chen, Yan Wang, Hongjian Liao, Ran Li, Hening Wang, Yihao Zhou, Hu Li, Jiajin Huang, Tongyu Zhang, Xu Ma, Hui |
author_facet | Chen, Yan Wang, Hongjian Liao, Ran Li, Hening Wang, Yihao Zhou, Hu Li, Jiajin Huang, Tongyu Zhang, Xu Ma, Hui |
author_sort | Chen, Yan |
collection | PubMed |
description | Suspended particles play a vital role in aquatic environments. We propose a method to rapidly measure the scattered polarization parameters of individual suspended particles with continuously large angular range (PCLAR), from 60° to 120° in one shot. A conceptual setup is built to measure PCLAR with 20 kHz; to verify the setup, 10 μm-diameter silica microspheres suspended in water, whose PCLAR are consistent with those simulated by Mie theory, are measured. PCLAR of 6 categories of particles are measured, which enables high-accuracy classification with the help of a convolutional neural network algorithm. PCLAR of different mixtures of Cyclotella stelligera and silica microspheres are measured to successfully identify particulate components. Furthermore, classification ability comparisons of different angular-selection strategies show that PCLAR enables the best classification beyond the single angle, discrete angles and small-ranged angles. Simulated PCLAR of particles with different size, refractive index, and structure show explicit discriminations between them. Inversely, the measured PCLAR are able to estimate the effective size and refractive index of individual Cyclotella cells. Results demonstrate the method’s power, which intrinsically takes the advantage of the optical polarization and the angular coverage. Future prototypes based on this concept would be a promising biosensor for particles in environmental monitoring. |
format | Online Article Text |
id | pubmed-9138884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91388842022-05-28 Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range Chen, Yan Wang, Hongjian Liao, Ran Li, Hening Wang, Yihao Zhou, Hu Li, Jiajin Huang, Tongyu Zhang, Xu Ma, Hui Biosensors (Basel) Article Suspended particles play a vital role in aquatic environments. We propose a method to rapidly measure the scattered polarization parameters of individual suspended particles with continuously large angular range (PCLAR), from 60° to 120° in one shot. A conceptual setup is built to measure PCLAR with 20 kHz; to verify the setup, 10 μm-diameter silica microspheres suspended in water, whose PCLAR are consistent with those simulated by Mie theory, are measured. PCLAR of 6 categories of particles are measured, which enables high-accuracy classification with the help of a convolutional neural network algorithm. PCLAR of different mixtures of Cyclotella stelligera and silica microspheres are measured to successfully identify particulate components. Furthermore, classification ability comparisons of different angular-selection strategies show that PCLAR enables the best classification beyond the single angle, discrete angles and small-ranged angles. Simulated PCLAR of particles with different size, refractive index, and structure show explicit discriminations between them. Inversely, the measured PCLAR are able to estimate the effective size and refractive index of individual Cyclotella cells. Results demonstrate the method’s power, which intrinsically takes the advantage of the optical polarization and the angular coverage. Future prototypes based on this concept would be a promising biosensor for particles in environmental monitoring. MDPI 2022-05-10 /pmc/articles/PMC9138884/ /pubmed/35624622 http://dx.doi.org/10.3390/bios12050321 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Yan Wang, Hongjian Liao, Ran Li, Hening Wang, Yihao Zhou, Hu Li, Jiajin Huang, Tongyu Zhang, Xu Ma, Hui Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range |
title | Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range |
title_full | Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range |
title_fullStr | Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range |
title_full_unstemmed | Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range |
title_short | Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range |
title_sort | rapidly measuring scattered polarization parameters of the individual suspended particle with continuously large angular range |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138884/ https://www.ncbi.nlm.nih.gov/pubmed/35624622 http://dx.doi.org/10.3390/bios12050321 |
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