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Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range

Suspended particles play a vital role in aquatic environments. We propose a method to rapidly measure the scattered polarization parameters of individual suspended particles with continuously large angular range (PCLAR), from 60° to 120° in one shot. A conceptual setup is built to measure PCLAR with...

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Detalles Bibliográficos
Autores principales: Chen, Yan, Wang, Hongjian, Liao, Ran, Li, Hening, Wang, Yihao, Zhou, Hu, Li, Jiajin, Huang, Tongyu, Zhang, Xu, Ma, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138884/
https://www.ncbi.nlm.nih.gov/pubmed/35624622
http://dx.doi.org/10.3390/bios12050321
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author Chen, Yan
Wang, Hongjian
Liao, Ran
Li, Hening
Wang, Yihao
Zhou, Hu
Li, Jiajin
Huang, Tongyu
Zhang, Xu
Ma, Hui
author_facet Chen, Yan
Wang, Hongjian
Liao, Ran
Li, Hening
Wang, Yihao
Zhou, Hu
Li, Jiajin
Huang, Tongyu
Zhang, Xu
Ma, Hui
author_sort Chen, Yan
collection PubMed
description Suspended particles play a vital role in aquatic environments. We propose a method to rapidly measure the scattered polarization parameters of individual suspended particles with continuously large angular range (PCLAR), from 60° to 120° in one shot. A conceptual setup is built to measure PCLAR with 20 kHz; to verify the setup, 10 μm-diameter silica microspheres suspended in water, whose PCLAR are consistent with those simulated by Mie theory, are measured. PCLAR of 6 categories of particles are measured, which enables high-accuracy classification with the help of a convolutional neural network algorithm. PCLAR of different mixtures of Cyclotella stelligera and silica microspheres are measured to successfully identify particulate components. Furthermore, classification ability comparisons of different angular-selection strategies show that PCLAR enables the best classification beyond the single angle, discrete angles and small-ranged angles. Simulated PCLAR of particles with different size, refractive index, and structure show explicit discriminations between them. Inversely, the measured PCLAR are able to estimate the effective size and refractive index of individual Cyclotella cells. Results demonstrate the method’s power, which intrinsically takes the advantage of the optical polarization and the angular coverage. Future prototypes based on this concept would be a promising biosensor for particles in environmental monitoring.
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spelling pubmed-91388842022-05-28 Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range Chen, Yan Wang, Hongjian Liao, Ran Li, Hening Wang, Yihao Zhou, Hu Li, Jiajin Huang, Tongyu Zhang, Xu Ma, Hui Biosensors (Basel) Article Suspended particles play a vital role in aquatic environments. We propose a method to rapidly measure the scattered polarization parameters of individual suspended particles with continuously large angular range (PCLAR), from 60° to 120° in one shot. A conceptual setup is built to measure PCLAR with 20 kHz; to verify the setup, 10 μm-diameter silica microspheres suspended in water, whose PCLAR are consistent with those simulated by Mie theory, are measured. PCLAR of 6 categories of particles are measured, which enables high-accuracy classification with the help of a convolutional neural network algorithm. PCLAR of different mixtures of Cyclotella stelligera and silica microspheres are measured to successfully identify particulate components. Furthermore, classification ability comparisons of different angular-selection strategies show that PCLAR enables the best classification beyond the single angle, discrete angles and small-ranged angles. Simulated PCLAR of particles with different size, refractive index, and structure show explicit discriminations between them. Inversely, the measured PCLAR are able to estimate the effective size and refractive index of individual Cyclotella cells. Results demonstrate the method’s power, which intrinsically takes the advantage of the optical polarization and the angular coverage. Future prototypes based on this concept would be a promising biosensor for particles in environmental monitoring. MDPI 2022-05-10 /pmc/articles/PMC9138884/ /pubmed/35624622 http://dx.doi.org/10.3390/bios12050321 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yan
Wang, Hongjian
Liao, Ran
Li, Hening
Wang, Yihao
Zhou, Hu
Li, Jiajin
Huang, Tongyu
Zhang, Xu
Ma, Hui
Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range
title Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range
title_full Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range
title_fullStr Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range
title_full_unstemmed Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range
title_short Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range
title_sort rapidly measuring scattered polarization parameters of the individual suspended particle with continuously large angular range
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138884/
https://www.ncbi.nlm.nih.gov/pubmed/35624622
http://dx.doi.org/10.3390/bios12050321
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