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Identification of Exosomal microRNAs and Their Targets in Papillary Thyroid Cancer Cells

The release of molecules in exosomal cargoes is involved in tumor development and progression. We compared the profiles of exosomal microRNAs released by two thyroid cancer cell lines (TPC-1 and K1) with that of non-tumorigenic thyroid cells (Nthy-ori-3-1), and we explored the network of miRNA–targe...

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Autores principales: Maggisano, Valentina, Capriglione, Francesca, Verrienti, Antonella, Celano, Marilena, Gagliardi, Agnese, Bulotta, Stefania, Sponziello, Marialuisa, Mio, Catia, Pecce, Valeria, Durante, Cosimo, Damante, Giuseppe, Russo, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138952/
https://www.ncbi.nlm.nih.gov/pubmed/35625697
http://dx.doi.org/10.3390/biomedicines10050961
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author Maggisano, Valentina
Capriglione, Francesca
Verrienti, Antonella
Celano, Marilena
Gagliardi, Agnese
Bulotta, Stefania
Sponziello, Marialuisa
Mio, Catia
Pecce, Valeria
Durante, Cosimo
Damante, Giuseppe
Russo, Diego
author_facet Maggisano, Valentina
Capriglione, Francesca
Verrienti, Antonella
Celano, Marilena
Gagliardi, Agnese
Bulotta, Stefania
Sponziello, Marialuisa
Mio, Catia
Pecce, Valeria
Durante, Cosimo
Damante, Giuseppe
Russo, Diego
author_sort Maggisano, Valentina
collection PubMed
description The release of molecules in exosomal cargoes is involved in tumor development and progression. We compared the profiles of exosomal microRNAs released by two thyroid cancer cell lines (TPC-1 and K1) with that of non-tumorigenic thyroid cells (Nthy-ori-3-1), and we explored the network of miRNA–target interaction. After extraction and characterization of exosomes, expression levels of microRNAs were investigated using custom TaqMan Advanced array cards, and compared with those expressed in the total cell extracts. The functional enrichment and network-based analysis of the miRNAs’ targets was also performed. Five microRNAs (miR-21-5p, miR-31-5p, miR-221-3p, miR-222-3p, and let-7i-3p) were significantly deregulated in the exosomes of tumor cells vs. non-tumorigenic cells, and three of them (miR-31-5p, miR-222-3p, and let-7i-3p) in the more aggressive K1 compared to TPC-1 cells. The network analysis of the five miRNAs identified some genes as targets of more than one miRNAs. These findings permitted the identification of exosomal microRNAs secreted by aggressive PTC cells, and indicated that their main targets are regulators of the tumor microenvironment. A deeper analysis of the functional role of the targets of exosomal miRNAs will provide further information on novel targets of molecular treatments for these neoplasms.
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spelling pubmed-91389522022-05-28 Identification of Exosomal microRNAs and Their Targets in Papillary Thyroid Cancer Cells Maggisano, Valentina Capriglione, Francesca Verrienti, Antonella Celano, Marilena Gagliardi, Agnese Bulotta, Stefania Sponziello, Marialuisa Mio, Catia Pecce, Valeria Durante, Cosimo Damante, Giuseppe Russo, Diego Biomedicines Article The release of molecules in exosomal cargoes is involved in tumor development and progression. We compared the profiles of exosomal microRNAs released by two thyroid cancer cell lines (TPC-1 and K1) with that of non-tumorigenic thyroid cells (Nthy-ori-3-1), and we explored the network of miRNA–target interaction. After extraction and characterization of exosomes, expression levels of microRNAs were investigated using custom TaqMan Advanced array cards, and compared with those expressed in the total cell extracts. The functional enrichment and network-based analysis of the miRNAs’ targets was also performed. Five microRNAs (miR-21-5p, miR-31-5p, miR-221-3p, miR-222-3p, and let-7i-3p) were significantly deregulated in the exosomes of tumor cells vs. non-tumorigenic cells, and three of them (miR-31-5p, miR-222-3p, and let-7i-3p) in the more aggressive K1 compared to TPC-1 cells. The network analysis of the five miRNAs identified some genes as targets of more than one miRNAs. These findings permitted the identification of exosomal microRNAs secreted by aggressive PTC cells, and indicated that their main targets are regulators of the tumor microenvironment. A deeper analysis of the functional role of the targets of exosomal miRNAs will provide further information on novel targets of molecular treatments for these neoplasms. MDPI 2022-04-21 /pmc/articles/PMC9138952/ /pubmed/35625697 http://dx.doi.org/10.3390/biomedicines10050961 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maggisano, Valentina
Capriglione, Francesca
Verrienti, Antonella
Celano, Marilena
Gagliardi, Agnese
Bulotta, Stefania
Sponziello, Marialuisa
Mio, Catia
Pecce, Valeria
Durante, Cosimo
Damante, Giuseppe
Russo, Diego
Identification of Exosomal microRNAs and Their Targets in Papillary Thyroid Cancer Cells
title Identification of Exosomal microRNAs and Their Targets in Papillary Thyroid Cancer Cells
title_full Identification of Exosomal microRNAs and Their Targets in Papillary Thyroid Cancer Cells
title_fullStr Identification of Exosomal microRNAs and Their Targets in Papillary Thyroid Cancer Cells
title_full_unstemmed Identification of Exosomal microRNAs and Their Targets in Papillary Thyroid Cancer Cells
title_short Identification of Exosomal microRNAs and Their Targets in Papillary Thyroid Cancer Cells
title_sort identification of exosomal micrornas and their targets in papillary thyroid cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138952/
https://www.ncbi.nlm.nih.gov/pubmed/35625697
http://dx.doi.org/10.3390/biomedicines10050961
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