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Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect

The gold standard for the treatment of peripheral nerve injuries, the autograft, presents several drawbacks, and engineered constructs are currently suitable only for short gaps or small diameter nerves. Here, we study a novel tissue-engineered multimodular nerve guidance conduit for the treatment o...

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Autores principales: Roca, Fernando Gisbert, Santos, Luis Gil, Roig, Manuel Mata, Medina, Lara Milian, Martínez-Ramos, Cristina, Pradas, Manuel Monleón
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138968/
https://www.ncbi.nlm.nih.gov/pubmed/35625700
http://dx.doi.org/10.3390/biomedicines10050963
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author Roca, Fernando Gisbert
Santos, Luis Gil
Roig, Manuel Mata
Medina, Lara Milian
Martínez-Ramos, Cristina
Pradas, Manuel Monleón
author_facet Roca, Fernando Gisbert
Santos, Luis Gil
Roig, Manuel Mata
Medina, Lara Milian
Martínez-Ramos, Cristina
Pradas, Manuel Monleón
author_sort Roca, Fernando Gisbert
collection PubMed
description The gold standard for the treatment of peripheral nerve injuries, the autograft, presents several drawbacks, and engineered constructs are currently suitable only for short gaps or small diameter nerves. Here, we study a novel tissue-engineered multimodular nerve guidance conduit for the treatment of large nerve damages based in a polylactic acid (PLA) microfibrillar structure inserted inside several co-linear hyaluronic acid (HA) conduits. The highly aligned PLA microfibers provide a topographical cue that guides axonal growth, and the HA conduits play the role of an epineurium and retain the pre-seeded auxiliary cells. The multimodular design increases the flexibility of the device. Its performance for the regeneration of a critical-size (15 mm) rabbit sciatic nerve defect was studied and, after six months, very good nerve regeneration was observed. The multimodular approach contributed to a better vascularization through the micrometrical gaps between HA conduits, and the pre-seeded Schwann cells increased axonal growth. Six months after surgery, a cross-sectional available area occupied by myelinated nerve fibers above 65% at the central and distal portions was obtained when the multimodular device with pre-seeded Schwann cells was employed. The results validate the multi-module approach for the regeneration of large nerve defects and open new possibilities for surgical solutions in this field.
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spelling pubmed-91389682022-05-28 Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect Roca, Fernando Gisbert Santos, Luis Gil Roig, Manuel Mata Medina, Lara Milian Martínez-Ramos, Cristina Pradas, Manuel Monleón Biomedicines Article The gold standard for the treatment of peripheral nerve injuries, the autograft, presents several drawbacks, and engineered constructs are currently suitable only for short gaps or small diameter nerves. Here, we study a novel tissue-engineered multimodular nerve guidance conduit for the treatment of large nerve damages based in a polylactic acid (PLA) microfibrillar structure inserted inside several co-linear hyaluronic acid (HA) conduits. The highly aligned PLA microfibers provide a topographical cue that guides axonal growth, and the HA conduits play the role of an epineurium and retain the pre-seeded auxiliary cells. The multimodular design increases the flexibility of the device. Its performance for the regeneration of a critical-size (15 mm) rabbit sciatic nerve defect was studied and, after six months, very good nerve regeneration was observed. The multimodular approach contributed to a better vascularization through the micrometrical gaps between HA conduits, and the pre-seeded Schwann cells increased axonal growth. Six months after surgery, a cross-sectional available area occupied by myelinated nerve fibers above 65% at the central and distal portions was obtained when the multimodular device with pre-seeded Schwann cells was employed. The results validate the multi-module approach for the regeneration of large nerve defects and open new possibilities for surgical solutions in this field. MDPI 2022-04-21 /pmc/articles/PMC9138968/ /pubmed/35625700 http://dx.doi.org/10.3390/biomedicines10050963 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Roca, Fernando Gisbert
Santos, Luis Gil
Roig, Manuel Mata
Medina, Lara Milian
Martínez-Ramos, Cristina
Pradas, Manuel Monleón
Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_full Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_fullStr Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_full_unstemmed Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_short Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_sort novel tissue-engineered multimodular hyaluronic acid-polylactic acid conduits for the regeneration of sciatic nerve defect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138968/
https://www.ncbi.nlm.nih.gov/pubmed/35625700
http://dx.doi.org/10.3390/biomedicines10050963
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