Cargando…

Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota

Gut microbes have been recognized to convert human bile acids by deconjugation, dehydroxylation, dehydrogenation, and epimerization of the cholesterol core, but the ability to re-conjugate them with amino acids as an additional conversion has been recently described. These new bile acids are known a...

Descripción completa

Detalles Bibliográficos
Autores principales: Garcia, Carlos J., Kosek, Vit, Beltrán, David, Tomás-Barberán, Francisco A., Hajslova, Jana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9139144/
https://www.ncbi.nlm.nih.gov/pubmed/35625615
http://dx.doi.org/10.3390/biom12050687
_version_ 1784714790373949440
author Garcia, Carlos J.
Kosek, Vit
Beltrán, David
Tomás-Barberán, Francisco A.
Hajslova, Jana
author_facet Garcia, Carlos J.
Kosek, Vit
Beltrán, David
Tomás-Barberán, Francisco A.
Hajslova, Jana
author_sort Garcia, Carlos J.
collection PubMed
description Gut microbes have been recognized to convert human bile acids by deconjugation, dehydroxylation, dehydrogenation, and epimerization of the cholesterol core, but the ability to re-conjugate them with amino acids as an additional conversion has been recently described. These new bile acids are known as microbially conjugated bile acids (MCBAs). The aim of this study was to evaluate the MCBAs diversity produced by the gut microbiota through a metabolomics approach. In this study, fresh fecal samples from healthy donors were evaluated to explore the re-conjugation of chenodeoxycholic and 3-oxo-chenodeoxycholic acids by the human gut microbiota. No significant differences were found between the conversion trend of both BAs incubations. The in vitro results showed a clear trend to first accumulate the epimer isoursochenodeoxycholic acid and the dehydroxylated lithocholic acid derivatives in samples incubated with chenodeoxycholic and 3-oxo-chenodeoxycholic acid. They also showed a strong trend for the production of microbially conjugated dehydroxylated bile acids instead of chenodeoxycholic backbone conjugates. Different molecules and isomers of MCBAs were identified, and the new ones, valolithocholate ester and leucolithocholate ester, were identified and confirmed by MS/MS. These results document the gut microbiota’s capability to produce esters of MCBAs on hydroxyls of the sterol backbone in addition to amides at the C24 acyl site. This study opens a new perspective to study the BAs diversity produced by the human gut microbiota.
format Online
Article
Text
id pubmed-9139144
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91391442022-05-28 Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota Garcia, Carlos J. Kosek, Vit Beltrán, David Tomás-Barberán, Francisco A. Hajslova, Jana Biomolecules Article Gut microbes have been recognized to convert human bile acids by deconjugation, dehydroxylation, dehydrogenation, and epimerization of the cholesterol core, but the ability to re-conjugate them with amino acids as an additional conversion has been recently described. These new bile acids are known as microbially conjugated bile acids (MCBAs). The aim of this study was to evaluate the MCBAs diversity produced by the gut microbiota through a metabolomics approach. In this study, fresh fecal samples from healthy donors were evaluated to explore the re-conjugation of chenodeoxycholic and 3-oxo-chenodeoxycholic acids by the human gut microbiota. No significant differences were found between the conversion trend of both BAs incubations. The in vitro results showed a clear trend to first accumulate the epimer isoursochenodeoxycholic acid and the dehydroxylated lithocholic acid derivatives in samples incubated with chenodeoxycholic and 3-oxo-chenodeoxycholic acid. They also showed a strong trend for the production of microbially conjugated dehydroxylated bile acids instead of chenodeoxycholic backbone conjugates. Different molecules and isomers of MCBAs were identified, and the new ones, valolithocholate ester and leucolithocholate ester, were identified and confirmed by MS/MS. These results document the gut microbiota’s capability to produce esters of MCBAs on hydroxyls of the sterol backbone in addition to amides at the C24 acyl site. This study opens a new perspective to study the BAs diversity produced by the human gut microbiota. MDPI 2022-05-11 /pmc/articles/PMC9139144/ /pubmed/35625615 http://dx.doi.org/10.3390/biom12050687 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garcia, Carlos J.
Kosek, Vit
Beltrán, David
Tomás-Barberán, Francisco A.
Hajslova, Jana
Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota
title Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota
title_full Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota
title_fullStr Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota
title_full_unstemmed Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota
title_short Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota
title_sort production of new microbially conjugated bile acids by human gut microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9139144/
https://www.ncbi.nlm.nih.gov/pubmed/35625615
http://dx.doi.org/10.3390/biom12050687
work_keys_str_mv AT garciacarlosj productionofnewmicrobiallyconjugatedbileacidsbyhumangutmicrobiota
AT kosekvit productionofnewmicrobiallyconjugatedbileacidsbyhumangutmicrobiota
AT beltrandavid productionofnewmicrobiallyconjugatedbileacidsbyhumangutmicrobiota
AT tomasbarberanfranciscoa productionofnewmicrobiallyconjugatedbileacidsbyhumangutmicrobiota
AT hajslovajana productionofnewmicrobiallyconjugatedbileacidsbyhumangutmicrobiota