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Influences of Long-Term Exercise and High-Fat Diet on Age-Related Telomere Shortening in Rats
(1) Obesity and exercise are believed to modify age-related telomere shortening by regulating telomerase and shelterins. Existing studies are inconsistent and limited to peripheral blood mononuclear cells (PBMCs) and selected solid tissues. (2) Female Sprague Dawley (SD) rats received either standar...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9139508/ https://www.ncbi.nlm.nih.gov/pubmed/35626642 http://dx.doi.org/10.3390/cells11101605 |
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author | Semeraro, Maria Donatella Almer, Gunter Renner, Wilfried Gruber, Hans-Jürgen Herrmann, Markus |
author_facet | Semeraro, Maria Donatella Almer, Gunter Renner, Wilfried Gruber, Hans-Jürgen Herrmann, Markus |
author_sort | Semeraro, Maria Donatella |
collection | PubMed |
description | (1) Obesity and exercise are believed to modify age-related telomere shortening by regulating telomerase and shelterins. Existing studies are inconsistent and limited to peripheral blood mononuclear cells (PBMCs) and selected solid tissues. (2) Female Sprague Dawley (SD) rats received either standard diet (ND) or high-fat diet (HFD). For 10 months, half of the animals from both diet groups performed 30 min running at 30 cm/s on five consecutive days followed by two days of rest (exeND, exeHFD). The remaining animals served as sedentary controls (coND, coHFD). Relative telomere length (RTL) and mRNA expression of telomerase (TERT) and the shelterins TERF-1 and TERF-2 were mapped in PBMCs and nine solid tissues. (3) At study end, coND and coHFD animals showed comparable RTL in most tissues with no systematic differences in TERT, TERF-1 and TERF-2 expression. Only visceral fat of coHFD animals showed reduced RTL and lower expression of TERT, TERF-1 and TERF-2. Exercise had heterogeneous effects on RTL in exeND and exeHFD animals with longer telomeres in aorta and large intestine, but shorter telomeres in PBMCs and liver. Telomere-regulating genes showed inconsistent expression patterns. (4) In conclusion, regular exercise or HFD cannot systematically modify RTL by regulating the expression of telomerase and shelterins. |
format | Online Article Text |
id | pubmed-9139508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91395082022-05-28 Influences of Long-Term Exercise and High-Fat Diet on Age-Related Telomere Shortening in Rats Semeraro, Maria Donatella Almer, Gunter Renner, Wilfried Gruber, Hans-Jürgen Herrmann, Markus Cells Article (1) Obesity and exercise are believed to modify age-related telomere shortening by regulating telomerase and shelterins. Existing studies are inconsistent and limited to peripheral blood mononuclear cells (PBMCs) and selected solid tissues. (2) Female Sprague Dawley (SD) rats received either standard diet (ND) or high-fat diet (HFD). For 10 months, half of the animals from both diet groups performed 30 min running at 30 cm/s on five consecutive days followed by two days of rest (exeND, exeHFD). The remaining animals served as sedentary controls (coND, coHFD). Relative telomere length (RTL) and mRNA expression of telomerase (TERT) and the shelterins TERF-1 and TERF-2 were mapped in PBMCs and nine solid tissues. (3) At study end, coND and coHFD animals showed comparable RTL in most tissues with no systematic differences in TERT, TERF-1 and TERF-2 expression. Only visceral fat of coHFD animals showed reduced RTL and lower expression of TERT, TERF-1 and TERF-2. Exercise had heterogeneous effects on RTL in exeND and exeHFD animals with longer telomeres in aorta and large intestine, but shorter telomeres in PBMCs and liver. Telomere-regulating genes showed inconsistent expression patterns. (4) In conclusion, regular exercise or HFD cannot systematically modify RTL by regulating the expression of telomerase and shelterins. MDPI 2022-05-10 /pmc/articles/PMC9139508/ /pubmed/35626642 http://dx.doi.org/10.3390/cells11101605 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Semeraro, Maria Donatella Almer, Gunter Renner, Wilfried Gruber, Hans-Jürgen Herrmann, Markus Influences of Long-Term Exercise and High-Fat Diet on Age-Related Telomere Shortening in Rats |
title | Influences of Long-Term Exercise and High-Fat Diet on Age-Related Telomere Shortening in Rats |
title_full | Influences of Long-Term Exercise and High-Fat Diet on Age-Related Telomere Shortening in Rats |
title_fullStr | Influences of Long-Term Exercise and High-Fat Diet on Age-Related Telomere Shortening in Rats |
title_full_unstemmed | Influences of Long-Term Exercise and High-Fat Diet on Age-Related Telomere Shortening in Rats |
title_short | Influences of Long-Term Exercise and High-Fat Diet on Age-Related Telomere Shortening in Rats |
title_sort | influences of long-term exercise and high-fat diet on age-related telomere shortening in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9139508/ https://www.ncbi.nlm.nih.gov/pubmed/35626642 http://dx.doi.org/10.3390/cells11101605 |
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