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In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up

Paclitaxel and trastuzumab have been associated with adverse effects including chemotherapy-induced peripheral neuropathy (CIPN) or ocular complications. In vivo confocal laser scanning microscopy (CLSM) of the cornea could be suitable for assessing side effects since the cornea is susceptible to, i...

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Autores principales: Bohn, Sebastian, Stache, Nadine, Sperlich, Karsten, Allgeier, Stephan, Köhler, Bernd, Bartschat, Andreas, Do, Ha-Vy, George, Christian, Guthoff, Rudolf F., Stachs, Angrit, Stachs, Oliver, Sterenczak, Katharina Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9139605/
https://www.ncbi.nlm.nih.gov/pubmed/35626335
http://dx.doi.org/10.3390/diagnostics12051180
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author Bohn, Sebastian
Stache, Nadine
Sperlich, Karsten
Allgeier, Stephan
Köhler, Bernd
Bartschat, Andreas
Do, Ha-Vy
George, Christian
Guthoff, Rudolf F.
Stachs, Angrit
Stachs, Oliver
Sterenczak, Katharina Anna
author_facet Bohn, Sebastian
Stache, Nadine
Sperlich, Karsten
Allgeier, Stephan
Köhler, Bernd
Bartschat, Andreas
Do, Ha-Vy
George, Christian
Guthoff, Rudolf F.
Stachs, Angrit
Stachs, Oliver
Sterenczak, Katharina Anna
author_sort Bohn, Sebastian
collection PubMed
description Paclitaxel and trastuzumab have been associated with adverse effects including chemotherapy-induced peripheral neuropathy (CIPN) or ocular complications. In vivo confocal laser scanning microscopy (CLSM) of the cornea could be suitable for assessing side effects since the cornea is susceptible to, i.e., neurotoxic stimuli. The study represents a one-year follow-up of a breast cancer patient including large-area in vivo CLSM of the subbasal nerve plexus (SNP), nerve function testing, and questionnaires during paclitaxel and trastuzumab therapy. Six monitoring sessions (one baseline, four during, and one after therapy) over 58 weeks were carried out. Large-area mosaics of the SNP were generated, and identical regions within all sessions were assigned. While corneal nerve morphology did not cause alterations, the number of dendritic cells (DCs) showed dynamic changes with a local burst at 11 weeks after baseline. Simultaneously, paclitaxel treatment was terminated due to side effects, which, together with DCs, returned to normal levels as the therapy progressed. Longitudinal in vivo CLSM of the SNP could complement routine examinations and be helpful to generate a comprehensive clinical picture. The applied techniques, with corneal structures acting as biomarkers could represent a diagnostic tool for the objective assessment of the severity of adverse events and the outcome.
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spelling pubmed-91396052022-05-28 In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up Bohn, Sebastian Stache, Nadine Sperlich, Karsten Allgeier, Stephan Köhler, Bernd Bartschat, Andreas Do, Ha-Vy George, Christian Guthoff, Rudolf F. Stachs, Angrit Stachs, Oliver Sterenczak, Katharina Anna Diagnostics (Basel) Interesting Images Paclitaxel and trastuzumab have been associated with adverse effects including chemotherapy-induced peripheral neuropathy (CIPN) or ocular complications. In vivo confocal laser scanning microscopy (CLSM) of the cornea could be suitable for assessing side effects since the cornea is susceptible to, i.e., neurotoxic stimuli. The study represents a one-year follow-up of a breast cancer patient including large-area in vivo CLSM of the subbasal nerve plexus (SNP), nerve function testing, and questionnaires during paclitaxel and trastuzumab therapy. Six monitoring sessions (one baseline, four during, and one after therapy) over 58 weeks were carried out. Large-area mosaics of the SNP were generated, and identical regions within all sessions were assigned. While corneal nerve morphology did not cause alterations, the number of dendritic cells (DCs) showed dynamic changes with a local burst at 11 weeks after baseline. Simultaneously, paclitaxel treatment was terminated due to side effects, which, together with DCs, returned to normal levels as the therapy progressed. Longitudinal in vivo CLSM of the SNP could complement routine examinations and be helpful to generate a comprehensive clinical picture. The applied techniques, with corneal structures acting as biomarkers could represent a diagnostic tool for the objective assessment of the severity of adverse events and the outcome. MDPI 2022-05-09 /pmc/articles/PMC9139605/ /pubmed/35626335 http://dx.doi.org/10.3390/diagnostics12051180 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Interesting Images
Bohn, Sebastian
Stache, Nadine
Sperlich, Karsten
Allgeier, Stephan
Köhler, Bernd
Bartschat, Andreas
Do, Ha-Vy
George, Christian
Guthoff, Rudolf F.
Stachs, Angrit
Stachs, Oliver
Sterenczak, Katharina Anna
In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up
title In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up
title_full In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up
title_fullStr In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up
title_full_unstemmed In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up
title_short In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up
title_sort in vivo monitoring of corneal dendritic cells in the subbasal nerve plexus during trastuzumab and paclitaxel breast cancer therapy—a one-year follow-up
topic Interesting Images
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9139605/
https://www.ncbi.nlm.nih.gov/pubmed/35626335
http://dx.doi.org/10.3390/diagnostics12051180
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