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Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats

Super-resolution ultrasound imaging, based on the localization and tracking of single intravascular microbubbles, makes it possible to map vessels below 100 µm. Microbubble velocities can be estimated as a surrogate for blood velocity, but their clinical potential is unclear. We investigated if a de...

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Autores principales: Andersen, Sofie Bech, Taghavi, Iman, Søgaard, Stinne Byrholdt, Hoyos, Carlos Armando Villagómez, Nielsen, Michael Bachmann, Jensen, Jørgen Arendt, Sørensen, Charlotte Mehlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140053/
https://www.ncbi.nlm.nih.gov/pubmed/35626267
http://dx.doi.org/10.3390/diagnostics12051111
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author Andersen, Sofie Bech
Taghavi, Iman
Søgaard, Stinne Byrholdt
Hoyos, Carlos Armando Villagómez
Nielsen, Michael Bachmann
Jensen, Jørgen Arendt
Sørensen, Charlotte Mehlin
author_facet Andersen, Sofie Bech
Taghavi, Iman
Søgaard, Stinne Byrholdt
Hoyos, Carlos Armando Villagómez
Nielsen, Michael Bachmann
Jensen, Jørgen Arendt
Sørensen, Charlotte Mehlin
author_sort Andersen, Sofie Bech
collection PubMed
description Super-resolution ultrasound imaging, based on the localization and tracking of single intravascular microbubbles, makes it possible to map vessels below 100 µm. Microbubble velocities can be estimated as a surrogate for blood velocity, but their clinical potential is unclear. We investigated if a decrease in microbubble velocity in the arterial and venous beds of the renal cortex, outer medulla, and inner medulla was detectable after intravenous administration of the α1-adrenoceptor antagonist prazosin. The left kidneys of seven rats were scanned with super-resolution ultrasound for 10 min before, during, and after prazosin administration using a bk5000 ultrasound scanner and hockey-stick probe. The super-resolution images were manually segmented, separating cortex, outer medulla, and inner medulla. Microbubble tracks from arteries/arterioles were separated from vein/venule tracks using the arterial blood flow direction. The mean microbubble velocities from each scan were compared. This showed a significant prazosin-induced velocity decrease only in the cortical arteries/arterioles (from 1.59 ± 0.38 to 1.14 ± 0.31 to 1.18 ± 0.33 mm/s, p = 0.013) and outer medulla descending vasa recta (from 0.70 ± 0.05 to 0.66 ± 0.04 to 0.69 ± 0.06 mm/s, p = 0.026). Conclusively, super-resolution ultrasound imaging makes it possible to detect and differentiate microbubble velocity responses to prazosin simultaneously in the renal cortical and medullary vascular beds.
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spelling pubmed-91400532022-05-28 Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats Andersen, Sofie Bech Taghavi, Iman Søgaard, Stinne Byrholdt Hoyos, Carlos Armando Villagómez Nielsen, Michael Bachmann Jensen, Jørgen Arendt Sørensen, Charlotte Mehlin Diagnostics (Basel) Article Super-resolution ultrasound imaging, based on the localization and tracking of single intravascular microbubbles, makes it possible to map vessels below 100 µm. Microbubble velocities can be estimated as a surrogate for blood velocity, but their clinical potential is unclear. We investigated if a decrease in microbubble velocity in the arterial and venous beds of the renal cortex, outer medulla, and inner medulla was detectable after intravenous administration of the α1-adrenoceptor antagonist prazosin. The left kidneys of seven rats were scanned with super-resolution ultrasound for 10 min before, during, and after prazosin administration using a bk5000 ultrasound scanner and hockey-stick probe. The super-resolution images were manually segmented, separating cortex, outer medulla, and inner medulla. Microbubble tracks from arteries/arterioles were separated from vein/venule tracks using the arterial blood flow direction. The mean microbubble velocities from each scan were compared. This showed a significant prazosin-induced velocity decrease only in the cortical arteries/arterioles (from 1.59 ± 0.38 to 1.14 ± 0.31 to 1.18 ± 0.33 mm/s, p = 0.013) and outer medulla descending vasa recta (from 0.70 ± 0.05 to 0.66 ± 0.04 to 0.69 ± 0.06 mm/s, p = 0.026). Conclusively, super-resolution ultrasound imaging makes it possible to detect and differentiate microbubble velocity responses to prazosin simultaneously in the renal cortical and medullary vascular beds. MDPI 2022-04-28 /pmc/articles/PMC9140053/ /pubmed/35626267 http://dx.doi.org/10.3390/diagnostics12051111 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Andersen, Sofie Bech
Taghavi, Iman
Søgaard, Stinne Byrholdt
Hoyos, Carlos Armando Villagómez
Nielsen, Michael Bachmann
Jensen, Jørgen Arendt
Sørensen, Charlotte Mehlin
Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats
title Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats
title_full Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats
title_fullStr Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats
title_full_unstemmed Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats
title_short Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats
title_sort super-resolution ultrasound imaging can quantify alterations in microbubble velocities in the renal vasculature of rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140053/
https://www.ncbi.nlm.nih.gov/pubmed/35626267
http://dx.doi.org/10.3390/diagnostics12051111
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