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Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis

The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to...

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Autores principales: Ramírez, Daniel, Rodríguez, María Esther, Cross, Ismael, Arias-Pérez, Alberto, Merlo, Manuel Alejandro, Anaya, Marco, Portela-Bens, Silvia, Martínez, Paulino, Robles, Francisca, Ruiz-Rejón, Carmelo, Rebordinos, Laureana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140517/
https://www.ncbi.nlm.nih.gov/pubmed/35628170
http://dx.doi.org/10.3390/ijms23105353
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author Ramírez, Daniel
Rodríguez, María Esther
Cross, Ismael
Arias-Pérez, Alberto
Merlo, Manuel Alejandro
Anaya, Marco
Portela-Bens, Silvia
Martínez, Paulino
Robles, Francisca
Ruiz-Rejón, Carmelo
Rebordinos, Laureana
author_facet Ramírez, Daniel
Rodríguez, María Esther
Cross, Ismael
Arias-Pérez, Alberto
Merlo, Manuel Alejandro
Anaya, Marco
Portela-Bens, Silvia
Martínez, Paulino
Robles, Francisca
Ruiz-Rejón, Carmelo
Rebordinos, Laureana
author_sort Ramírez, Daniel
collection PubMed
description The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5–9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions.
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spelling pubmed-91405172022-05-28 Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis Ramírez, Daniel Rodríguez, María Esther Cross, Ismael Arias-Pérez, Alberto Merlo, Manuel Alejandro Anaya, Marco Portela-Bens, Silvia Martínez, Paulino Robles, Francisca Ruiz-Rejón, Carmelo Rebordinos, Laureana Int J Mol Sci Article The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5–9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions. MDPI 2022-05-11 /pmc/articles/PMC9140517/ /pubmed/35628170 http://dx.doi.org/10.3390/ijms23105353 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramírez, Daniel
Rodríguez, María Esther
Cross, Ismael
Arias-Pérez, Alberto
Merlo, Manuel Alejandro
Anaya, Marco
Portela-Bens, Silvia
Martínez, Paulino
Robles, Francisca
Ruiz-Rejón, Carmelo
Rebordinos, Laureana
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
title Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
title_full Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
title_fullStr Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
title_full_unstemmed Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
title_short Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
title_sort integration of maps enables a cytogenomics analysis of the complete karyotype in solea senegalensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140517/
https://www.ncbi.nlm.nih.gov/pubmed/35628170
http://dx.doi.org/10.3390/ijms23105353
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