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Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls

Photomorphogenic responses of etiolated seedlings include the inhibition of hypocotyl elongation and opening of the apical hook. In addition, dark-grown seedlings respond to light by the formation of adventitious roots (AR) on the hypocotyl. How light signaling controls adventitious rooting is less...

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Autores principales: Zeng, Yinwei, Schotte, Sebastien, Trinh, Hoang Khai, Verstraeten, Inge, Li, Jing, Van de Velde, Ellen, Vanneste, Steffen, Geelen, Danny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140560/
https://www.ncbi.nlm.nih.gov/pubmed/35628112
http://dx.doi.org/10.3390/ijms23105301
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author Zeng, Yinwei
Schotte, Sebastien
Trinh, Hoang Khai
Verstraeten, Inge
Li, Jing
Van de Velde, Ellen
Vanneste, Steffen
Geelen, Danny
author_facet Zeng, Yinwei
Schotte, Sebastien
Trinh, Hoang Khai
Verstraeten, Inge
Li, Jing
Van de Velde, Ellen
Vanneste, Steffen
Geelen, Danny
author_sort Zeng, Yinwei
collection PubMed
description Photomorphogenic responses of etiolated seedlings include the inhibition of hypocotyl elongation and opening of the apical hook. In addition, dark-grown seedlings respond to light by the formation of adventitious roots (AR) on the hypocotyl. How light signaling controls adventitious rooting is less well understood. Hereto, we analyzed adventitious rooting under different light conditions in wild type and photomorphogenesis mutants in Arabidopsis thaliana. Etiolation was not essential for AR formation but raised the competence to form AR under white and blue light. The blue light receptors CRY1 and PHOT1/PHOT2 are key elements contributing to the induction of AR formation in response to light. Furthermore, etiolation-controlled competence for AR formation depended on the COP9 signalosome, E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC (COP1), the COP1 interacting SUPPRESSOR OF PHYA-105 (SPA) kinase family members (SPA1,2 and 3) and Phytochrome-Interacting Factors (PIF). In contrast, ELONGATED HYPOCOTYL5 (HY5), suppressed AR formation. These findings provide a genetic framework that explains the high and low AR competence of Arabidopsis thaliana hypocotyls that were treated with dark, and light, respectively. We propose that light-induced auxin signal dissipation generates a transient auxin maximum that explains AR induction by a dark to light switch.
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spelling pubmed-91405602022-05-28 Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls Zeng, Yinwei Schotte, Sebastien Trinh, Hoang Khai Verstraeten, Inge Li, Jing Van de Velde, Ellen Vanneste, Steffen Geelen, Danny Int J Mol Sci Article Photomorphogenic responses of etiolated seedlings include the inhibition of hypocotyl elongation and opening of the apical hook. In addition, dark-grown seedlings respond to light by the formation of adventitious roots (AR) on the hypocotyl. How light signaling controls adventitious rooting is less well understood. Hereto, we analyzed adventitious rooting under different light conditions in wild type and photomorphogenesis mutants in Arabidopsis thaliana. Etiolation was not essential for AR formation but raised the competence to form AR under white and blue light. The blue light receptors CRY1 and PHOT1/PHOT2 are key elements contributing to the induction of AR formation in response to light. Furthermore, etiolation-controlled competence for AR formation depended on the COP9 signalosome, E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC (COP1), the COP1 interacting SUPPRESSOR OF PHYA-105 (SPA) kinase family members (SPA1,2 and 3) and Phytochrome-Interacting Factors (PIF). In contrast, ELONGATED HYPOCOTYL5 (HY5), suppressed AR formation. These findings provide a genetic framework that explains the high and low AR competence of Arabidopsis thaliana hypocotyls that were treated with dark, and light, respectively. We propose that light-induced auxin signal dissipation generates a transient auxin maximum that explains AR induction by a dark to light switch. MDPI 2022-05-10 /pmc/articles/PMC9140560/ /pubmed/35628112 http://dx.doi.org/10.3390/ijms23105301 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zeng, Yinwei
Schotte, Sebastien
Trinh, Hoang Khai
Verstraeten, Inge
Li, Jing
Van de Velde, Ellen
Vanneste, Steffen
Geelen, Danny
Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls
title Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls
title_full Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls
title_fullStr Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls
title_full_unstemmed Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls
title_short Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls
title_sort genetic dissection of light-regulated adventitious root induction in arabidopsis thaliana hypocotyls
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140560/
https://www.ncbi.nlm.nih.gov/pubmed/35628112
http://dx.doi.org/10.3390/ijms23105301
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