The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil

Flavonoids are significant antioxidant and anti-inflammatory agents and have multiple potential health applications. Moringa oleifera is globally recognized for its nutritional and pharmacological properties, correlated to the high flavonoid content in its leaves. However, the bioactive compounds fo...

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Autores principales: Silva, Larissa Marina Pereira, Inácio, Maria Raquel Cavalcanti, da Silva, Gualter Guenter Costa, Silva, Jucier Magson de Souza e, da Luz, Jefferson Romáryo Duarte, Almeida, Maria das Graças, Moraes, Edgar Perin, Esposito, Debora, Ferreira, Leandro De Santis, Zucolotto, Silvana Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140588/
https://www.ncbi.nlm.nih.gov/pubmed/35627022
http://dx.doi.org/10.3390/foods11101452
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author Silva, Larissa Marina Pereira
Inácio, Maria Raquel Cavalcanti
da Silva, Gualter Guenter Costa
Silva, Jucier Magson de Souza e
da Luz, Jefferson Romáryo Duarte
Almeida, Maria das Graças
Moraes, Edgar Perin
Esposito, Debora
Ferreira, Leandro De Santis
Zucolotto, Silvana Maria
author_facet Silva, Larissa Marina Pereira
Inácio, Maria Raquel Cavalcanti
da Silva, Gualter Guenter Costa
Silva, Jucier Magson de Souza e
da Luz, Jefferson Romáryo Duarte
Almeida, Maria das Graças
Moraes, Edgar Perin
Esposito, Debora
Ferreira, Leandro De Santis
Zucolotto, Silvana Maria
author_sort Silva, Larissa Marina Pereira
collection PubMed
description Flavonoids are significant antioxidant and anti-inflammatory agents and have multiple potential health applications. Moringa oleifera is globally recognized for its nutritional and pharmacological properties, correlated to the high flavonoid content in its leaves. However, the bioactive compounds found in plants may vary according to the cultivation, origin, season, and extraction process used, making it difficult to extract reliable raw material. Hence, this study aimed to standardize the best cultivation and harvest season in Brazil and the best extraction process conditions to obtain a flavonoid-rich extract from M. oleifera as a final product. Firstly, ultrasound-assisted extraction (UAE) was optimized to reach the highest flavonoid content by three-level factorial planning and response surface methodology (RSM). The optimal cultivation condition was mineral soil fertilizer in the drought season, and the optimized extraction was with 80% ethanol and 13.4 min of extraction time. The flavonoid-rich extract was safe and significantly decreased reactive oxygen species (ROS) and nitric oxide (NO) in LPS-treated RAW 264.7 cells. Lastly, the major flavonoids characterized by HPLC-ESI-QTRAP-MS/MS were compounds derived from apigenin, quercetin, and kaempferol glycosides. The results confirmed that it was possible to standardize the flavonoid-rich extract leading to a standardized and reliable raw material extracted from M. oleifera leaves.
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spelling pubmed-91405882022-05-28 The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil Silva, Larissa Marina Pereira Inácio, Maria Raquel Cavalcanti da Silva, Gualter Guenter Costa Silva, Jucier Magson de Souza e da Luz, Jefferson Romáryo Duarte Almeida, Maria das Graças Moraes, Edgar Perin Esposito, Debora Ferreira, Leandro De Santis Zucolotto, Silvana Maria Foods Article Flavonoids are significant antioxidant and anti-inflammatory agents and have multiple potential health applications. Moringa oleifera is globally recognized for its nutritional and pharmacological properties, correlated to the high flavonoid content in its leaves. However, the bioactive compounds found in plants may vary according to the cultivation, origin, season, and extraction process used, making it difficult to extract reliable raw material. Hence, this study aimed to standardize the best cultivation and harvest season in Brazil and the best extraction process conditions to obtain a flavonoid-rich extract from M. oleifera as a final product. Firstly, ultrasound-assisted extraction (UAE) was optimized to reach the highest flavonoid content by three-level factorial planning and response surface methodology (RSM). The optimal cultivation condition was mineral soil fertilizer in the drought season, and the optimized extraction was with 80% ethanol and 13.4 min of extraction time. The flavonoid-rich extract was safe and significantly decreased reactive oxygen species (ROS) and nitric oxide (NO) in LPS-treated RAW 264.7 cells. Lastly, the major flavonoids characterized by HPLC-ESI-QTRAP-MS/MS were compounds derived from apigenin, quercetin, and kaempferol glycosides. The results confirmed that it was possible to standardize the flavonoid-rich extract leading to a standardized and reliable raw material extracted from M. oleifera leaves. MDPI 2022-05-17 /pmc/articles/PMC9140588/ /pubmed/35627022 http://dx.doi.org/10.3390/foods11101452 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Larissa Marina Pereira
Inácio, Maria Raquel Cavalcanti
da Silva, Gualter Guenter Costa
Silva, Jucier Magson de Souza e
da Luz, Jefferson Romáryo Duarte
Almeida, Maria das Graças
Moraes, Edgar Perin
Esposito, Debora
Ferreira, Leandro De Santis
Zucolotto, Silvana Maria
The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil
title The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil
title_full The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil
title_fullStr The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil
title_full_unstemmed The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil
title_short The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil
title_sort first optimization process from cultivation to flavonoid-rich extract from moringa oleifera lam. leaves in brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140588/
https://www.ncbi.nlm.nih.gov/pubmed/35627022
http://dx.doi.org/10.3390/foods11101452
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