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Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116

Humantenine, an alkaloid isolated from the medicinal herb Gelsemium elegans (Gardner & Chapm.) Benth., has been reported to induce intestinal irritation, but the underlying toxicological mechanisms remain unclear. The object of the present study was to investigate the RNA N6-methyladenosine (m6A...

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Autores principales: Wu, Yajiao, Chen, Xiaoying, Bao, Wenqiang, Hong, Xinyu, Li, Chutao, Lu, Jiatong, Zhang, Dongcheng, Zhu, An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140730/
https://www.ncbi.nlm.nih.gov/pubmed/35627166
http://dx.doi.org/10.3390/genes13050781
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author Wu, Yajiao
Chen, Xiaoying
Bao, Wenqiang
Hong, Xinyu
Li, Chutao
Lu, Jiatong
Zhang, Dongcheng
Zhu, An
author_facet Wu, Yajiao
Chen, Xiaoying
Bao, Wenqiang
Hong, Xinyu
Li, Chutao
Lu, Jiatong
Zhang, Dongcheng
Zhu, An
author_sort Wu, Yajiao
collection PubMed
description Humantenine, an alkaloid isolated from the medicinal herb Gelsemium elegans (Gardner & Chapm.) Benth., has been reported to induce intestinal irritation, but the underlying toxicological mechanisms remain unclear. The object of the present study was to investigate the RNA N6-methyladenosine (m6A) modification and distinct mRNA transcriptome profiles in humantenine-treated HCT116 human colon cancer cells. High-throughput MeRIP-seq and mRNA-seq were performed, and bioinformatic analysis was performed to reveal the role of abnormal RNA m6A modification and mRNA expression in humantenine-induced intestinal cell toxicity. After humantenine treatment of HCT116 cells, 1401 genes were in the overlap of differentially m6A-modified mRNA and differentially expressed mRNA. The Kyoto Encyclopedia of Genes and Genomes and Gene Ontology annotation terms for actin cytoskeleton, tight junctions, and adherens junctions were enriched. A total of 11 kinds of RNA m6A methylation regulators were differentially expressed. The m6A methylation levels of target genes were disordered in the humantenine group. In conclusion, this study suggested that the HCT116 cell injury induced by humantenine was associated with the abnormal mRNA expression of m6A regulators, as well as disordered m6A methylation levels of target genes.
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spelling pubmed-91407302022-05-28 Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116 Wu, Yajiao Chen, Xiaoying Bao, Wenqiang Hong, Xinyu Li, Chutao Lu, Jiatong Zhang, Dongcheng Zhu, An Genes (Basel) Article Humantenine, an alkaloid isolated from the medicinal herb Gelsemium elegans (Gardner & Chapm.) Benth., has been reported to induce intestinal irritation, but the underlying toxicological mechanisms remain unclear. The object of the present study was to investigate the RNA N6-methyladenosine (m6A) modification and distinct mRNA transcriptome profiles in humantenine-treated HCT116 human colon cancer cells. High-throughput MeRIP-seq and mRNA-seq were performed, and bioinformatic analysis was performed to reveal the role of abnormal RNA m6A modification and mRNA expression in humantenine-induced intestinal cell toxicity. After humantenine treatment of HCT116 cells, 1401 genes were in the overlap of differentially m6A-modified mRNA and differentially expressed mRNA. The Kyoto Encyclopedia of Genes and Genomes and Gene Ontology annotation terms for actin cytoskeleton, tight junctions, and adherens junctions were enriched. A total of 11 kinds of RNA m6A methylation regulators were differentially expressed. The m6A methylation levels of target genes were disordered in the humantenine group. In conclusion, this study suggested that the HCT116 cell injury induced by humantenine was associated with the abnormal mRNA expression of m6A regulators, as well as disordered m6A methylation levels of target genes. MDPI 2022-04-27 /pmc/articles/PMC9140730/ /pubmed/35627166 http://dx.doi.org/10.3390/genes13050781 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Yajiao
Chen, Xiaoying
Bao, Wenqiang
Hong, Xinyu
Li, Chutao
Lu, Jiatong
Zhang, Dongcheng
Zhu, An
Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116
title Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116
title_full Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116
title_fullStr Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116
title_full_unstemmed Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116
title_short Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116
title_sort effect of humantenine on mrna m6a modification and expression in human colon cancer cell line hct116
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140730/
https://www.ncbi.nlm.nih.gov/pubmed/35627166
http://dx.doi.org/10.3390/genes13050781
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