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Identification of the CKM Gene as a Potential Muscle-Specific Safe Harbor Locus in Pig Genome

Genetically modified pigs have shown considerable application potential in the fields of life science research and livestock breeding. Nevertheless, a barrier impedes the production of genetically modified pigs. There are too few safe harbor loci for the insertion of foreign genes into the pig genom...

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Detalles Bibliográficos
Autores principales: Xiong, Youcai, Zhuang, Rongzhi, Zhao, Guangxing, Liu, Yanwen, Su, Yinyu, Wang, Wei, Xi, Xiaoning, Yang, Yanyu, Han, Xiaosong, Xie, Shengsong, Wang, Heng, Li, Xinyun, Zuo, Bo, Zhao, Shuhong, Feng, Zheng, Ruan, Jinxue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140944/
https://www.ncbi.nlm.nih.gov/pubmed/35627307
http://dx.doi.org/10.3390/genes13050921
Descripción
Sumario:Genetically modified pigs have shown considerable application potential in the fields of life science research and livestock breeding. Nevertheless, a barrier impedes the production of genetically modified pigs. There are too few safe harbor loci for the insertion of foreign genes into the pig genome. Only a few loci (pRosa26, pH11 and Pifs501) have been successfully identified to achieve the ectopic expression of foreign genes and produce gene-edited pigs. Here, we use CRISPR/Cas9-mediated homologous directed repair (HDR) to accurately knock the exogenous gene-of-interest fragments into an endogenous CKM gene in the porcine satellite cells. After porcine satellite cells are induced to differentiate, the CKM gene promoter simultaneously initiates the expression of the CKM gene and the exogenous gene. We infer preliminarily that the CKM gene can be identified as a potential muscle-specific safe harbor locus in pigs for the integration of exogenous gene-of-interest fragments.