Cargando…
Enhanced Soluble Expression of Linoleic Acid Isomerase by Coordinated Regulation of Promoter and Fusion Tag in Escherichia coli
PAI is a linoleic acid isomerase from Propionibacterium acnes and is the key enzyme in the synthesis of trans10, cis12-conjugated linoleic acid. However, the majority of the expressed PAI in Escherichia coli occurs in its nonfunctional form in inclusion bodies, limiting the biosynthesis of conjugate...
Autores principales: | Zhang, Baixi, Zhu, Tong, Huang, Xintian |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141242/ https://www.ncbi.nlm.nih.gov/pubmed/35627089 http://dx.doi.org/10.3390/foods11101515 |
Ejemplares similares
-
Heterologous expression of a novel linoleic acid isomerase BBI, and effect of fusion tags on its performance
por: Mei, Yongchao, et al.
Publicado: (2022) -
Fusion Tag Design Influences Soluble Recombinant Protein Production in Escherichia coli
por: Köppl, Christoph, et al.
Publicado: (2022) -
Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system
por: Costa, Sofia, et al.
Publicado: (2014) -
Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins
por: Cheng, Chung-Hsien, et al.
Publicado: (2010) -
The Protocatechuate 3,4-Dioxygenase Solubility (PCDS) Tag Enhances the Expression and Solubility of Heterogenous Proteins in Escherichia coli
por: Zou, Lei, et al.
Publicado: (2021)