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Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions
Ribosomal DNA (rDNA) is an excellent cytogenetic marker owing to its tandem arrangement and high copy numbers. However, comparative studies have focused more on the number of rDNA site variations within the Chrysanthemum genus, and studies on the types of rDNA sites with the same experimental proced...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141308/ https://www.ncbi.nlm.nih.gov/pubmed/35627279 http://dx.doi.org/10.3390/genes13050894 |
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author | He, Jun Zhao, Yong Zhang, Shuangshuang He, Yanze Jiang, Jiafu Chen, Sumei Fang, Weimin Guan, Zhiyong Liao, Yuan Wang, Zhenxing Chen, Fadi Wang, Haibin |
author_facet | He, Jun Zhao, Yong Zhang, Shuangshuang He, Yanze Jiang, Jiafu Chen, Sumei Fang, Weimin Guan, Zhiyong Liao, Yuan Wang, Zhenxing Chen, Fadi Wang, Haibin |
author_sort | He, Jun |
collection | PubMed |
description | Ribosomal DNA (rDNA) is an excellent cytogenetic marker owing to its tandem arrangement and high copy numbers. However, comparative studies have focused more on the number of rDNA site variations within the Chrysanthemum genus, and studies on the types of rDNA sites with the same experimental procedures at the species levels are lacking. To further explore the number and types of rDNA site variations, we combined related data to draw ideograms of the rDNA sites of Chrysanthemum accessions using oligonucleotide fluorescence in situ hybridization (Oligo-FISH). Latent variations (such as polymorphisms of 45S rDNA sites and co-localized 5S-45S rDNA) also occurred among the investigated accessions. Meanwhile, a significant correlation was observed between the number of 5S rDNA sites and chromosome number. Additionally, the clumped and concentrated geographical distribution of different ploidy Chrysanthemum accessions may significantly promote the karyotype evolution. Based on the results above, we identified the formation mechanism of rDNA variations. Furthermore, these findings may provide a reliable method to examine the sites and number of rDNA variations among Chrysanthemum and its related accessions and allow researchers to further understand the evolutionary and phylogenetic relationships of the Chrysanthemum genus. |
format | Online Article Text |
id | pubmed-9141308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91413082022-05-28 Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions He, Jun Zhao, Yong Zhang, Shuangshuang He, Yanze Jiang, Jiafu Chen, Sumei Fang, Weimin Guan, Zhiyong Liao, Yuan Wang, Zhenxing Chen, Fadi Wang, Haibin Genes (Basel) Article Ribosomal DNA (rDNA) is an excellent cytogenetic marker owing to its tandem arrangement and high copy numbers. However, comparative studies have focused more on the number of rDNA site variations within the Chrysanthemum genus, and studies on the types of rDNA sites with the same experimental procedures at the species levels are lacking. To further explore the number and types of rDNA site variations, we combined related data to draw ideograms of the rDNA sites of Chrysanthemum accessions using oligonucleotide fluorescence in situ hybridization (Oligo-FISH). Latent variations (such as polymorphisms of 45S rDNA sites and co-localized 5S-45S rDNA) also occurred among the investigated accessions. Meanwhile, a significant correlation was observed between the number of 5S rDNA sites and chromosome number. Additionally, the clumped and concentrated geographical distribution of different ploidy Chrysanthemum accessions may significantly promote the karyotype evolution. Based on the results above, we identified the formation mechanism of rDNA variations. Furthermore, these findings may provide a reliable method to examine the sites and number of rDNA variations among Chrysanthemum and its related accessions and allow researchers to further understand the evolutionary and phylogenetic relationships of the Chrysanthemum genus. MDPI 2022-05-17 /pmc/articles/PMC9141308/ /pubmed/35627279 http://dx.doi.org/10.3390/genes13050894 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article He, Jun Zhao, Yong Zhang, Shuangshuang He, Yanze Jiang, Jiafu Chen, Sumei Fang, Weimin Guan, Zhiyong Liao, Yuan Wang, Zhenxing Chen, Fadi Wang, Haibin Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions |
title | Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions |
title_full | Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions |
title_fullStr | Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions |
title_full_unstemmed | Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions |
title_short | Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions |
title_sort | uneven levels of 5s and 45s rdna site number and loci variations across wild chrysanthemum accessions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141308/ https://www.ncbi.nlm.nih.gov/pubmed/35627279 http://dx.doi.org/10.3390/genes13050894 |
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