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Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions

Ribosomal DNA (rDNA) is an excellent cytogenetic marker owing to its tandem arrangement and high copy numbers. However, comparative studies have focused more on the number of rDNA site variations within the Chrysanthemum genus, and studies on the types of rDNA sites with the same experimental proced...

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Autores principales: He, Jun, Zhao, Yong, Zhang, Shuangshuang, He, Yanze, Jiang, Jiafu, Chen, Sumei, Fang, Weimin, Guan, Zhiyong, Liao, Yuan, Wang, Zhenxing, Chen, Fadi, Wang, Haibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141308/
https://www.ncbi.nlm.nih.gov/pubmed/35627279
http://dx.doi.org/10.3390/genes13050894
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author He, Jun
Zhao, Yong
Zhang, Shuangshuang
He, Yanze
Jiang, Jiafu
Chen, Sumei
Fang, Weimin
Guan, Zhiyong
Liao, Yuan
Wang, Zhenxing
Chen, Fadi
Wang, Haibin
author_facet He, Jun
Zhao, Yong
Zhang, Shuangshuang
He, Yanze
Jiang, Jiafu
Chen, Sumei
Fang, Weimin
Guan, Zhiyong
Liao, Yuan
Wang, Zhenxing
Chen, Fadi
Wang, Haibin
author_sort He, Jun
collection PubMed
description Ribosomal DNA (rDNA) is an excellent cytogenetic marker owing to its tandem arrangement and high copy numbers. However, comparative studies have focused more on the number of rDNA site variations within the Chrysanthemum genus, and studies on the types of rDNA sites with the same experimental procedures at the species levels are lacking. To further explore the number and types of rDNA site variations, we combined related data to draw ideograms of the rDNA sites of Chrysanthemum accessions using oligonucleotide fluorescence in situ hybridization (Oligo-FISH). Latent variations (such as polymorphisms of 45S rDNA sites and co-localized 5S-45S rDNA) also occurred among the investigated accessions. Meanwhile, a significant correlation was observed between the number of 5S rDNA sites and chromosome number. Additionally, the clumped and concentrated geographical distribution of different ploidy Chrysanthemum accessions may significantly promote the karyotype evolution. Based on the results above, we identified the formation mechanism of rDNA variations. Furthermore, these findings may provide a reliable method to examine the sites and number of rDNA variations among Chrysanthemum and its related accessions and allow researchers to further understand the evolutionary and phylogenetic relationships of the Chrysanthemum genus.
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spelling pubmed-91413082022-05-28 Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions He, Jun Zhao, Yong Zhang, Shuangshuang He, Yanze Jiang, Jiafu Chen, Sumei Fang, Weimin Guan, Zhiyong Liao, Yuan Wang, Zhenxing Chen, Fadi Wang, Haibin Genes (Basel) Article Ribosomal DNA (rDNA) is an excellent cytogenetic marker owing to its tandem arrangement and high copy numbers. However, comparative studies have focused more on the number of rDNA site variations within the Chrysanthemum genus, and studies on the types of rDNA sites with the same experimental procedures at the species levels are lacking. To further explore the number and types of rDNA site variations, we combined related data to draw ideograms of the rDNA sites of Chrysanthemum accessions using oligonucleotide fluorescence in situ hybridization (Oligo-FISH). Latent variations (such as polymorphisms of 45S rDNA sites and co-localized 5S-45S rDNA) also occurred among the investigated accessions. Meanwhile, a significant correlation was observed between the number of 5S rDNA sites and chromosome number. Additionally, the clumped and concentrated geographical distribution of different ploidy Chrysanthemum accessions may significantly promote the karyotype evolution. Based on the results above, we identified the formation mechanism of rDNA variations. Furthermore, these findings may provide a reliable method to examine the sites and number of rDNA variations among Chrysanthemum and its related accessions and allow researchers to further understand the evolutionary and phylogenetic relationships of the Chrysanthemum genus. MDPI 2022-05-17 /pmc/articles/PMC9141308/ /pubmed/35627279 http://dx.doi.org/10.3390/genes13050894 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Jun
Zhao, Yong
Zhang, Shuangshuang
He, Yanze
Jiang, Jiafu
Chen, Sumei
Fang, Weimin
Guan, Zhiyong
Liao, Yuan
Wang, Zhenxing
Chen, Fadi
Wang, Haibin
Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions
title Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions
title_full Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions
title_fullStr Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions
title_full_unstemmed Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions
title_short Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions
title_sort uneven levels of 5s and 45s rdna site number and loci variations across wild chrysanthemum accessions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141308/
https://www.ncbi.nlm.nih.gov/pubmed/35627279
http://dx.doi.org/10.3390/genes13050894
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