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Genome-Wide Identification and Expressional Profiling of the Metal Tolerance Protein Gene Family in Brassica napus
The Cation Diffusion Facilitator (CDF) family, also named Metal Tolerance Protein (MTP), is one of the gene families involved in heavy metal transport in plants. However, a comprehensive study of MTPs in Brassica napus has not been reported yet. In the present study, we identified 33 BnMTP genes fro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141485/ https://www.ncbi.nlm.nih.gov/pubmed/35627146 http://dx.doi.org/10.3390/genes13050761 |
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author | Xie, Tao Yang, Wenjing Chen, Xin Rong, Hao Wang, Youping Jiang, Jinjin |
author_facet | Xie, Tao Yang, Wenjing Chen, Xin Rong, Hao Wang, Youping Jiang, Jinjin |
author_sort | Xie, Tao |
collection | PubMed |
description | The Cation Diffusion Facilitator (CDF) family, also named Metal Tolerance Protein (MTP), is one of the gene families involved in heavy metal transport in plants. However, a comprehensive study of MTPs in Brassica napus has not been reported yet. In the present study, we identified 33 BnMTP genes from the rapeseed genome using bioinformatic analyses. Subsequently, we analyzed the phylogenetic relationship, gene structure, chromosome distribution, conserved domains, and motifs of the BnMTP gene family. The 33 BnMTPs were phylogenetically divided into three major clusters (Zn-CDFs, Fe/Zn-CDFs, and Mn-CDFs) and seven groups (group 1, 5, 6, 7, 8, 9, and 12). The structural characteristics of the BnMTP members were similar in the same group, but different among groups. Evolutionary analysis indicated that the BnMTP gene family mainly expanded through whole-genome duplication (WGD) and segmental duplication events. Moreover, the prediction of cis-acting elements and microRNA target sites suggested that BnMTPs might be involved in plant growth, development, and stress responses. In addition, we found the expression of 24 BnMTPs in rapeseed leaves or roots could respond to heavy metal ion treatments. These results provided an important basis for clarifying the biological functions of BnMTPs, especially in heavy metal detoxification, and will be helpful in the phytoremediation of heavy metal pollution in soil. |
format | Online Article Text |
id | pubmed-9141485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91414852022-05-28 Genome-Wide Identification and Expressional Profiling of the Metal Tolerance Protein Gene Family in Brassica napus Xie, Tao Yang, Wenjing Chen, Xin Rong, Hao Wang, Youping Jiang, Jinjin Genes (Basel) Article The Cation Diffusion Facilitator (CDF) family, also named Metal Tolerance Protein (MTP), is one of the gene families involved in heavy metal transport in plants. However, a comprehensive study of MTPs in Brassica napus has not been reported yet. In the present study, we identified 33 BnMTP genes from the rapeseed genome using bioinformatic analyses. Subsequently, we analyzed the phylogenetic relationship, gene structure, chromosome distribution, conserved domains, and motifs of the BnMTP gene family. The 33 BnMTPs were phylogenetically divided into three major clusters (Zn-CDFs, Fe/Zn-CDFs, and Mn-CDFs) and seven groups (group 1, 5, 6, 7, 8, 9, and 12). The structural characteristics of the BnMTP members were similar in the same group, but different among groups. Evolutionary analysis indicated that the BnMTP gene family mainly expanded through whole-genome duplication (WGD) and segmental duplication events. Moreover, the prediction of cis-acting elements and microRNA target sites suggested that BnMTPs might be involved in plant growth, development, and stress responses. In addition, we found the expression of 24 BnMTPs in rapeseed leaves or roots could respond to heavy metal ion treatments. These results provided an important basis for clarifying the biological functions of BnMTPs, especially in heavy metal detoxification, and will be helpful in the phytoremediation of heavy metal pollution in soil. MDPI 2022-04-26 /pmc/articles/PMC9141485/ /pubmed/35627146 http://dx.doi.org/10.3390/genes13050761 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xie, Tao Yang, Wenjing Chen, Xin Rong, Hao Wang, Youping Jiang, Jinjin Genome-Wide Identification and Expressional Profiling of the Metal Tolerance Protein Gene Family in Brassica napus |
title | Genome-Wide Identification and Expressional Profiling of the Metal Tolerance Protein Gene Family in Brassica napus |
title_full | Genome-Wide Identification and Expressional Profiling of the Metal Tolerance Protein Gene Family in Brassica napus |
title_fullStr | Genome-Wide Identification and Expressional Profiling of the Metal Tolerance Protein Gene Family in Brassica napus |
title_full_unstemmed | Genome-Wide Identification and Expressional Profiling of the Metal Tolerance Protein Gene Family in Brassica napus |
title_short | Genome-Wide Identification and Expressional Profiling of the Metal Tolerance Protein Gene Family in Brassica napus |
title_sort | genome-wide identification and expressional profiling of the metal tolerance protein gene family in brassica napus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141485/ https://www.ncbi.nlm.nih.gov/pubmed/35627146 http://dx.doi.org/10.3390/genes13050761 |
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